請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/32809
標題: Demonstration of thyrotropin receptor mRNA in orbital fat and eye muscle tissues from patients with Graves' ophthalmopathy by in situ hybridization
作者: Wu, S.L.
張天傑
Yang, C.S.J.
Wang, H.J.
Liao, C.L.
Chang, T.J.
Chang, T.C.
關鍵字: Graves' ophthalmopathy
thyrotropin receptor
orbital fat
eye muscle
in situ hybridization
thyroid-associated ophthalmopathy
tsh receptor
messenger-rna
retroocular fibroblasts
extracellular domain
cultured fibroblasts
pretibial dermopathy
gene-expression
binding-sites
cloning
期刊/報告no:: Journal of Endocrinological Investigation, Volume 22, Issue 4, Page(s) 289-295.
摘要: There is a controversy regarding whether there are thyrotropin (TSH) receptors in orbital fat and eye muscle tissues that may play a role in the pathogenesis of Graves' ophthalmopathy. To elucidate whether there are TSH receptors in orbital fat and eye muscle tissues in patients with Graves' ophthalmopathy, we applied the method of in situ hybridization in orbital fat and eye muscle tissues obtained during the operation for patients with Graves' ophthalmopathy, to directly detect TSH receptor mRNA. To identify whether the cells with positive TSH receptor mRNA are fibroblasts, we also did vimentin immunoreactivity study. To further prove the transcript does have a full length of TSH receptor, the samples of total RNA preparations, extracted from orbital fat and eye muscle tissues, were used as a template for reverse transcriptase polymerase chain reaction (RT-PCR) using three primer sets to generate cDNA fragments and cloned for sequencing. The results showed that the expression of TSH receptor mRNA was demonstrated in adipocytes and fibroblasts of orbital fat, and perimysial fibroblasts within eye muscle tissues by in situ hybridization and vimentin immunoreactivity study. Also, by using the RT-PCR, cloning and sequencing, we further proved that the transcript does have a full length of TSH receptor. The present study suggested that there are TSH receptors expressed in orbital fat and eye muscle tissues. (J. Endocrinol. Invest. 22: 289-295, 1999) (C)1999, Editrice Kurtis.
URI: http://hdl.handle.net/11455/32809
ISSN: 0391-4097
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