Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32824
標題: Examination of meat components in commercial dog and cat feed by using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) technique
作者: Wang, H.C.
張天傑
Lee, S.H.
Chang, T.J.
Wong, M.L.
關鍵字: BSE
cyt b gene
dog and cat commercial diet
PCR
polymorphism
species identification
protein profiles
fish
gene
期刊/報告no:: Journal of Veterinary Medical Science, Volume 66, Issue 7, Page(s) 855-859.
摘要: It has been shown that certain slow neurological diseases such as bovine spongiform encephalopathy (also known as "mad cow" disease) could be transmitted through contaminated food intake by animals; therefore, the examination of meat components in commercial feeds is important for the control of the disease in public health. The combination of polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) technique was applied to examine the meat components in dog and cat commercial feeds. The partial nucleotide sequence (359 bp) of animal mitochondrial cytochrome b (cytb, CYT) gene was amplified by PCR and then digested with restriction enzyme Alu I or Mbo I. In this work, eight brands of commercial dog and cat feeds available in Taiwan were examined. All brands of dog feeds that were tested contained meat from four different animals (cattle, pig, goat and chicken). In cat feeds, the chicken meat was found in five out of eight brands.
URI: http://hdl.handle.net/11455/32824
ISSN: 0916-7250
文章連結: http://dx.doi.org/10.1292/jvms.66.855
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