Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32848
標題: Expression of recombinant envelope protein of Japanese encephalitis virus YL strain in Escherichia coli possesses hemagglutination activity
作者: Chen, S.O.
張天傑
Tsai, T.H.
Chang, T.J.
Wong, M.L.
Su, H.P.
Liu, J.J.
關鍵字: envelope glycoprotein
hemagglutination
Japanese encephalitis virus
YL
strain
phylogenetic analysis
swiss-model
in-vitro
flavivirus
virulence
cloning
mutants
genome
cdna
mice
期刊/報告no:: Virus Genes, Volume 28, Issue 2, Page(s) 215-221.
摘要: The nucleotide sequence of glycoprotein E of YL vaccine strain was cloned, sequenced and expressed in E. coli. Phylogenetic analysis of envelope ( E) amino acid sequences of 18 JEVs in GenBank showed that the vaccine strain YL closer to the virulent strain HVI which is a Taiwanese isolate. We found only two amino acid mutations (K-138 and G-389) of E protein might lead viral attenuation in YL. In this study, we used pRSET vector system to construct three recombinant plasmids (pRSET/F1R1, pRSET/ F2R2 and pRSET/F1R2), which encoded and expressed different or overlapping amino acid region of E protein. The antigenicity and hemagglutination activity of these recombinant proteins were examined by western blotting and hemagglutination test, respectively. Our results demonstrated that the recombinant protein of pRSET/F1R2 possesses predominant antigenicity and hemagglutination activity.
URI: http://hdl.handle.net/11455/32848
ISSN: 0920-8569
文章連結: http://dx.doi.org/10.1023/B:VIRU.0000016861.78378.9b
Appears in Collections:獸醫學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.