請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/32850
標題: Recombinant core proteins of Japanese encephalitis virus as activators of the innate immune response
作者: Chen, S.O.
張天傑
Fang, S.H.
Shih, D.Y.
Chang, T.J.
Liu, J.J.
關鍵字: Japanese encephalitis virus
Core protein
Nitric oxide
toll-like receptors
envelope protein
capsid protein
nitric-oxide
yl-strain
infection
replication
localization
expression
death
期刊/報告no:: Virus Genes, Volume 38, Issue 1, Page(s) 10-18.
摘要: Nitric oxide (NO) has been shown to suppress Japanese encephalitis virus (JEV) RNA synthesis, viral protein accumulation, and virus release from infected cells. In this article, the potential viral structural proteins as the activators of NO product were studied at the molecular level. First, the genomic region encoding the JEV structural proteins was cloned into a prokaryotic expression vector pET for high-level expression. After purification, these JEV recombinant proteins were added to macrophages to examine the productions of NO and pro-inflammatory mediators. In this study, the recombinant core protein, but not envelope (E), could trigger NO and pro-inflammatory mediators (TNF-alpha, IL-6, and IL-12) productions on macrophages. And their effects were about 85-95% relative to LPS-stimulated macrophages in a dose-dependent manner. Meanwhile, the rCore-2D could up regulate promoters of IL-8 and TNF-alpha via EGFP expression in reporter plasmid (IL-8p-EGFP and TNF-alpha p-EGFP)-transfected cells by flow cytometric analysis. These results suggest that JEV core protein could regulate pro-inflammatory mediators and NO production, and may play a crucial role in the innate immunity for the host to restrict the initial stage of JEV infection.
URI: http://hdl.handle.net/11455/32850
ISSN: 0920-8569
文章連結: http://dx.doi.org/10.1007/s11262-008-0299-9
顯示於類別:獸醫學系所

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