Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32869
標題: Mice protected by oral immunization with Lactobacillus reuteri secreting fusion protein of Escherichia coli enterotoxin subunit protein
作者: Wu, C.M.
張登欽
Chung, T.C.
關鍵字: Escherichia coli enterotoxin
Lactobacillus reuteri
oral vaccine
green
fluorescent protein
green fluorescent protein
lactic-acid bacteria
nisin-controlled
expression
heat-labile enterotoxin
toxin fragment-c
tetanus toxin
b-subunit
gastrointestinal-tract
vectors
vaccine
期刊/報告no:: Fems Immunology and Medical Microbiology, Volume 50, Issue 3, Page(s) 354-365.
摘要: A green fluorescent protein (gfp) gene was ligated to the Lactobacillus reuteri-specific nisin-inducible expression-secretion vector pNIES, generating a pNIES-GFP vector capable of secreting the cloned gene as a GFP-fusion protein with fluorescent activity. To develop this system as a live vehicle carrying the heat-stable enterotoxin (ST) and heat-labile enterotoxin B (LTB) of the enterotoxigenic Escherichia coli (ETEC), a recombinant 5'-ST-LTB-3' DNA fragment was cloned into pNIES-GFP. The resulting L. reuteri/pNIES-GFP:STLTB system was found to possess the capability of adhering to the mice gut, secreting GFP:STLTB product at 0.14 and 0.026 pgcell(-1) under induced and noninduced conditions, respectively. Further analysis of the GFP:STLTB product confirmed its ganglioside-binding ability, LTB antigenicity and relative freedom from the ST-associated toxicity, making it suitable for use as an oral vaccine in mice. Oral inoculation of the L. reuteri/pNIES-GFP:STLTB culture in mice elicited significant (P < 0.01) serum IgG and mucosal IgA antibodies against the STLTB antigen. These immunized mice were subsequently challenged with ETEC and showed full protection against the fluid influx response in the gut. This is the first report of using L. reuteri as a vaccine carrier to induce complete immunologic protection against ETEC.
URI: http://hdl.handle.net/11455/32869
ISSN: 0928-8244
文章連結: http://dx.doi.org/10.1111/j.1574-695X.2007.00255.x
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