Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32876
標題: Green fluorescent protein is a reliable reporter for screening signal peptides functional in Lactobacillus routeri
作者: Wu, C.M.
張登欽
Chung, T.C.
關鍵字: green fluorescent protein
Lactobacillus reuteri
signal peptide-probe
vector
toxin fragment-c
lactococcus-lactis
alpha-amylase
tetanus toxin
cell-surface
expression
secretion
promoter
bacteria
sequence
期刊/報告no:: Journal of Microbiological Methods, Volume 67, Issue 1, Page(s) 181-186.
摘要: A signal peptide (SP)-probe vector pNICE-gfpSP, which employed a green fluorescent protein (Gfp) as the SP-selection marker, was constructed for use in Lactobacillus reuteri. This chimerical plasmid allowed cloning and screening DNA fragments with the SP function by direct visualization of the expressed fluorescence activity around cells. Assay of fluorescent intensity in their culture supernatant with spectrofluorometry further enabled quantifying the secretion efficiency of the identified SP fragment. (c) 2006 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/32876
ISSN: 0167-7012
文章連結: http://dx.doi.org/10.1016/j.mimet.2006.03.009
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