Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3294
標題: The Synthesis and Evaluation of Poly(carbonate)urethanes as Biomaterials
聚碳酸酯型聚胺酯生醫材料合成與其生物相容性之測試
作者: lin, zu zun
林自長
關鍵字: poly(ether)urethane
聚醚型聚胺酯
poly(carbonate)urethane
microphase seperation
blood compatibility
in vivo and in vitro biostability
albumin
fibrinogen
competitive adsorption
聚碳酸酯型聚胺酯
微相分離
血液相容性
體內和體外生物穩定性
白蛋白
纖維蛋白
競爭性吸附
出版社: 化學工程學系
摘要: In this research, a total of twelve poly(carbonate)urethanes (PCUs) were synthesized using four different soft macrodiols, and three hard/soft segment ratios. The four macrodiols include two from America CT Biomaterials (U.S.), as well as two from Japan Nippon Polyurethane Industry (Japan). The hard segment was 4,4'-disphenylmethane diisocyanate (MDI) and chain extender was 2-butene-1,4-diol (1,4-BDO). Dynamic mechanical analysis demonstrated larger microphase seperation for poly(carbonate)urethanes with higher soft segment ratio. In the same soft segment series, the surface of PCU containing more soft segment (greater degree of microphase seperation) was more hydrophlic (i.e. higher surface energy) and easier for endothelial to adhere and proliferate. In blood compatibility tests, PCU with greater degree of microphase and easier reduced the platelet adhesion and activation on the surface. This may be attributed to the preferential adsorption for albumin observed on these surfaces. Finally, in biostability tests for both in vitro (H2O2/Co2+) and in vivo (implantation in rats for three months), poly(carbonate)urethanes was found stable than poly(ether)urethanes. Therefore, poly(cabonate)urethanes with their superior blood compatibility, cellular adhesion, and biostability could be better candidates for cardiovascular biomaterials. We expect to employ them in fabrication of the small-diameter vascular grafts and artificial skins.
本研究主要從聚碳酸酯型聚胺酯(poly(carbonate)urethane; PCU)化學結構設計上,改變軟、硬鏈節的合成計量比,由此可改變PCU材料的微相分離程度(degree of microphase seperation)的差異。研究發現以MDI為硬鏈節、1,4-BDO(2-butene-1,4-diols)為鏈延伸劑,而軟鏈節部份則分別以美國分子量865、2017和日本分子量1000、2000的聚碳酸酯型多元醇為主時,發現用美國和日本的四種不同分子量的軟鏈節在PCU中的化學計量提高時,其微相分離程度也相對提高。 在聚碳酸酯型PCU的物性測量結果方面,由動態機械分析儀(DMA)的結果來分析, 可觀察到PCUJ1000、PCUJ2000 及PCUA865、PCUA2017四個系列的PCU中以PCU431J1000、PCU431J2000及PCU431A865、PCU431A2017的Tg在同組材料中最低;即其微相分離程度是同系列材料中較大者。同時,當軟鏈節化學計量提高,PCU的拉伸率也相對的提高。 在材料表面的接觸角及表面能測試結果方面,發現同系列聚碳酸酯型聚胺基甲酸酯材料中微相分離愈大者;材料愈親水、表面能愈大,較利於內皮細胞的貼附及生長。尤其,以PCUA2017系列在四個系列中的內皮細胞(endothelial cell; EC)貼附與生長情形最佳。同時,同系列碳酸酯型的PCU中微相分離較大者,其albumin對fibrinogen競爭吸附(competitive adsorption)量的比值會相對提高,同時也發現其血小板的吸附量相對較低,有較佳的血液相容性。 在生物穩定性方面,體外(in vitro)氧化實驗與老鼠皮下降解(in vivo)實驗均發現以聚碳酸酯型的多元醇為軟鏈節時,此類型PCU在生物體內的抗生物降解性較聚醚型系列PU高,即具有較佳的生物穩定性。 綜觀以上結果,本研究發現同系列軟鏈節的聚碳酸酯型聚胺酯材料中,微相分離愈大者,其細胞相容性、血液相容性及生物穩定性都有相對的增加趨勢。因此,像PCU431A2107、PCU431A865、PCU431J1000、PCU431J2000等微相分離較大的材料較適合用來當作生醫材料。 在未來可由化學合成設計上,選取合適的軟、硬鏈節及鏈延伸劑並改變軟、硬鏈節化學計量比,可得到較大微相分離程度的聚胺酯;如此,便可間接改善生醫材料的血液相容性、細胞相容性及生物穩定性等性質。 關鍵字:聚醚型聚胺酯、聚碳酸酯型聚胺酯、微相分離、血液相容性、體內和體外生物穩定性、球蛋白、纖維蛋白、血小板、競爭性吸附。
URI: http://hdl.handle.net/11455/3294
Appears in Collections:化學工程學系所

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