請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/32974
標題: Descending facilitation of spinal NMDA-dependent reflex potentiation from pontine tegmentum in rats
作者: Chen, G.D.
董光中
Peng, H.Y.
Tung, K.C.
Cheng, C.L.
Chen, Y.J.
Liao, J.M.
Ho, Y.C.
Pan, S.F.
Chen, M.J.
Lin, T.B.
關鍵字: long term potentiation
SRP
pontine tegmentum
serotonin
WAY 100635
8-OH-DPAT
long-term potentiation
external urethral sphincter
lower
urinary-tract
micturition-reflex
anesthetized rats
synaptic-transmission
binding-sites
nerve injury
dorsal-horn
alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid
期刊/報告no:: American Journal of Physiology-Renal Physiology, Volume 293, Issue 4, Page(s) F1115-F1122.
摘要: This study was conducted to investigate whether dorsolateral pontine tegmentum stimulation modulates spinal reflex potentiation (SRP) and whether serotonergic neurotransmission is involved in such a modulation. Reflex activities of the external urethra sphincter (EUS) electromyogram in response to a test stimulation (TS; 1/30 Hz) or repetitive stimulation (RS; 1 Hz) on the pelvic afferent nerve in 35 anesthetized rats were recorded with/without synchronized train pontine stimulation (PS; 300 Hz, 30 ms) and/or intrathecal administrations of 10 mu l of 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo (F) quinoxaline (NBQX; 100 mu M), D-2-amino-5-phosphonovalerate (APV; 100 mu M), N-[2-[4-( 2-methoxyphenyl)-1-piperazinyl]ethyl]-N-(2-pyridinyl) cyclohexanecarboxamide trihydrochloride (WAY 100635; 100 mu M), and 8-hydroxy-2-( di-n-propylamino)-tetralin (8-OH-DPAT; 100 mu M). The TS evoked a single action potential (1.00 +/- 0.00 spikes/stimulation), while the RS produced a long-lasting SRP (16.12 +/- 1.59 spikes/stimulation) that was abolished by APV (1.57 +/- 0.29 spikes/stimulation) and was attenuated by NBQX (7.42 +/- 0.57 spikes/stimulation). Synchronized train PS with RS (PS+RS) produced facilitation in RS-induced SRP (25.17 +/- 2.21 spikes/stimulation). Intrathecal WAY 100635 abolished the facilitation in SRP as a result of the synchronized PS (14.66 +/- 1.58 spikes/stimulation). On the other hand, intrathecal 8-OH-DPAT elicited facilitation in the RS-induced SRP (25.16 +/- 1.05 spikes/ stimulation) without synchronized PS. Our findings suggest that dorsolateral pontine tegmentum may modulate N-methyl-D-aspartic acid-dependent SRP via descending serotonergic neurotransmission. This descending modulation may have physiological/pharmacological relevance in the neural controls of urethral closure.
URI: http://hdl.handle.net/11455/32974
ISSN: 1931-857X
文章連結: http://dx.doi.org/10.1152/ajprenal.00135.2007
顯示於類別:獸醫學系所

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