Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32975
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dc.contributor.authorWu, H.C.en_US
dc.contributor.author董光中zh_TW
dc.contributor.authorChiu, C.H.en_US
dc.contributor.authorTung, K.C.en_US
dc.contributor.authorChen, G.D.en_US
dc.contributor.authorPeng, H.Y.en_US
dc.contributor.authorLin, T.B.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-06T07:44:32Z-
dc.date.available2014-06-06T07:44:32Z-
dc.identifier.issn1931-857Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/32975-
dc.description.abstractWu HC, Chiu CH, Tung KC, Chen GD, Peng HY, Lin TB. Dopaminergic D2 receptors activate PKA to inhibit spinal pelvic-urethra reflex in rats. Am J Physiol Renal Physiol 299: F681-F686, 2010. First published June 16, 2010; doi:10.1152/ajprenal.00090.2010.-To clarify the role of descending dopaminergic innervation in reflexive urethral closure, the impacts of dopaminergic D2 receptor (DR2)-selective agonists and antagonists on repetitive stimulation-induced pelvic-to-urethra spinal reflex potentiation (SRP) were tested using in vivo rat preparations. Pelvic afferent nerve test stimulation (TS; 1 pulse/30 s for 30 min) evoked baseline reflex activity with single spikes in the external urethral sphincter electromyogram (EUSE), whereas, repetitive stimulation (RS; 1 pulse/s for 30 min) induced SRP. Intrathecal application of quinelorane dihydrochloride (Q110; 10, 30, and 100 nM, 10 mu l, a selective DR2 agonist) dose dependently inhibited the RS-induced SRP. Pretreatment with L135 (100 nM, 10 mu L it, a selective DR2 antagonist) antagonized the Q110-dependent inhibition (100 nM, 10 mu l it). Intrathecal AMPA (10 mu M, 10 mu l, a selective glutamatergic AMPA receptor agonist), and NMDA (10 mu M, 10 mu l, a selective glutamatergic NMDA receptor agonist) reversed the Q110-dependent inhibition. Intrathecal forskolin (100 nM, 10 mu l, a PKA activator) prevented the Q110-dependent inhibition that was reversed by CNQX (10 mu M, 10 mu l it, a selective glutamate AMPA receptor antagonist) and APV (10 mu M, 10 mu l it, a selective glutamate NMDA receptor antagonist). Our results suggest that DR2 activation, which inactivates intracellular PKA, may be involved in descending dopaminergic inhibition of NMDA/AMPA receptor-dependent SRP at the lumbosacral spinal cord, which is thought to be involved in reflexive urethral closure.en_US
dc.language.isoen_USzh_TW
dc.relationAmerican Journal of Physiology-Renal Physiologyen_US
dc.relation.ispartofseriesAmerican Journal of Physiology-Renal Physiology, Volume 299, Issue 3, Page(s) F681-F686.en_US
dc.relation.urihttp://dx.doi.org/10.1152/ajprenal.00090.2010en_US
dc.subjectD2 receptoren_US
dc.subjectcAMPen_US
dc.subjecturethra closureen_US
dc.subjectQ110en_US
dc.subjectL135en_US
dc.subjectraten_US
dc.subjectcerebral-artery occlusionen_US
dc.subjectcross-organ sensitizationen_US
dc.subjectsynapticen_US
dc.subjectplasticityen_US
dc.subjectmicturition reflexen_US
dc.subjectsubstantia-nigraen_US
dc.subjectdorsal-hornen_US
dc.subjectcorden_US
dc.subjectmechanismsen_US
dc.subjectphosphorylationen_US
dc.subjectneuronsen_US
dc.titleDopaminergic D2 receptors activate PKA to inhibit spinal pelvic-urethra reflex in ratsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1152/ajprenal.00090.2010zh_TW
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