Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32976
標題: Endogenous ephrinB2 mediates colon-urethra cross-organ sensitization via Src kinase-dependent tyrosine phosphorylation of NR2B
作者: Peng, H.Y.
董光中
Chen, G.D.
Lai, C.H.
Tung, K.C.
Chang, J.L.
Lin, T.B.
關鍵字: pelvic pain syndrome
urethra
irritable bowel syndrome
colon
NMDA
spinal reflex potentiation
nmda receptor regulation
d-aspartate
receptor
inflammatory hyperalgesia
synaptic plasticity
neuropathic
pain
ephb receptors
subunit
rats
maintenance
期刊/報告no:: American Journal of Physiology-Renal Physiology, Volume 298, Issue 1, Page(s) F109-F117.
摘要: Peng H, Chen G, Lai C, Tung K, Chang J, Lin T. Endogenous ephrinB2 mediates colon-urethra cross-organ sensitization via Src kinase-dependent tyrosine phosphorylation of NR2B. Am J Physiol Renal Physiol 298: F109-F117, 2010. First published October 28, 2009; doi:10.1152/ajprenal.00287.2009.-Recently, the role of EphB receptor (EphBR) tyrosine kinase and their ephrinB ligands in spinal pain-related neural plasticity has been identified. To test whether Src-family non-receptor tyrosine kinase-dependent glutamatergic N-methyl-D-aspartate receptor (NMDAR) NR2B subunit phosphorylation underlies lumbosacral spinal EphBR activation to mediate cross-organ sensitization between the colon and the urethra, external urethra sphincter electromyogram activity evoked by pelvic nerve stimulation and protein expression in the lumbosacral (L6-S2) dorsal horn were studied before and after intracolonic mustard oil (MO) instillation. We found MO instillation produced colon-urethra reflex sensitization along with an upregulation of endogenous ephrinB2 expression as well as phosphorylation of EphB1/2, Src-family kinase, and NR2B tyrosine residues. Intrathecal immunoglobulin fusion protein of EphB1 and EphB2 as well as PP2 reversed the reflex sensitization and NR2B phosphorylation caused by MO. All these results suggest that EphBR-ephrinB interactions, which provoke Src-family kinase-dependent NMDAR NR2B phosphorylation at the lumbosacral spinal cord level, are involved in cross-organ sensitization, contributing to the development of viscero-visceral referred pain between the bowel and the urethra.
URI: http://hdl.handle.net/11455/32976
ISSN: 1931-857X
文章連結: http://dx.doi.org/10.1152/ajprenal.00287.2009
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