Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32989
標題: E4BP4 is a cardiac survival factor and essential for embryonic heart development
作者: Weng, Y.J.
董光中
Hsieh, D.J.Y.
Kuo, W.W.
Lai, T.Y.
Hsu, H.H.
Tsai, C.H.
Tsai, F.J.
Lin, D.Y.
Lin, J.A.
Huang, C.Y.
Tung, K.C.
關鍵字: E4BP4
Heart development
Cardiac survival factor
transcriptional repression
hematopoietic-cells
bzip family
myocardial-infarction
gene-expression
factor-ii
zebrafish
model
toxicity
growth
期刊/報告no:: Molecular and Cellular Biochemistry, Volume 340, Issue 1-2, Page(s) 187-194.
摘要: The bZIP transcription factor E4BP4, has been demonstrated to be a survival factor in pro-B lymphocytes. GATA factors play important roles in transducing the IL-3 survival signal and transactivating the downstream survival gene, E4BP4. In heart, GATA sites are essential for proper transcription of several cardiac genes, and GATA-4 is a mediator of cardiomyocyte survival. However, the role E4BP4 plays in heart is still poorly understood. In this study, Dot-blot hybridization assays using Dig-labeled RNA probes revealed that the E4BP4 gene was expressed in cardiac tissue from several species including, monkey, dog, rabbit, and human. Western blot analysis showed that the E4BP4 protein was consistently present in all of these four species. Furthermore, immunohistochemistry revealed that the E4BP4 protein was overexpressed in diseased heart tissue in comparison with normal heart tissue. In addition, the overexpression of E4BP4 in vitro activated cell survival signaling pathway of cardiomyocytes. At last, siRNA-mediated knock down of E4BP4 in zebrafish resulted in malformed looping of the embryonic heart tube and decreased heart beating. Based on these results, we conclude that E4BP4 plays as a survival factor in heart and E4BP4 is essential for proper embryonic heart development.
URI: http://hdl.handle.net/11455/32989
ISSN: 0300-8177
文章連結: http://dx.doi.org/10.1007/s11010-010-0417-6
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