請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/32990
標題: Glutamate-mediated spinal reflex potentiation involves ERK 1/2 phosphorylation in anesthetized rats
作者: Peng, H.Y.
董光中
Cheng, Y.W.
Lee, S.D.
Ho, Y.C.
Chou, D.
Chen, G.D.
Cheng, C.L.
Hsu, T.H.
Tung, K.C.
Lin, T.B.
關鍵字: extracellular signal-regulated kinase
phosphorylation
protein kinase
A
protein kinase C
glutamate
activated protein-kinase
long-term potentiation
element-binding
protein
signal-regulated kinase
dorsal-horn neurons
urinary-bladder
contraction
fiber-evoked potentials
erk integrates pka
map kinase
in-vivo
期刊/報告no:: Neuropharmacology, Volume 54, Issue 4, Page(s) 686-698.
摘要: The extracellular signal-regulated kinase (ERK) cascades are suggested to contribute to excitatory plasticity in the CNS, including the spinal cord. This study investigated whether the ERK involves in the repetitive stimulation-induced spinal reflex potentiation (SRP) in the pelvic nerveto-external urethra sphincter reflex activities. External urethra sphincter electromyogram in response to pelvic afferent nerve test stimulation (TS, 1/30 Hz) or repetitive stimulation (RS, 1 Hz) was recorded in anesthetized rats. TS evoked a baseline reflex activity, whereas RS produced SRP in associated with significant ERK 1/2 phosphorylation. RS-induced SRP and ERK 1/2 phosphorylation were both abolished by pretreatment of U0126 (MEK inhibitor). Intrathecal CNQX (AMPA receptor antagonist) attenuated, while AP5 (NMDA receptor antagonist) abolished the RS-induced SRP and ERK 1/2 phosphorylation. Pretreated U0126 abolished the SRP elicited by glutamatergic agonists including glutamate, NMDA and AMPA. Intrathecal H89 and BIS7 (PKA and PKC inhibitors, respectively) both abolished the RS- and glutamate agonist-induced SRP as well as ERK 1/2 phosphorylation. In addition, forskolin and PMA (PKA and PKC activator, respectively) induced SRP, which were both abolished by pretreated U0126. Saline distension, mimicking the storage phase of the urinary bladder, induced SRP and ERK 1/2 phosphorylation. In conclusion. activated ERK 1/2 may produce SRP in the pelvic nerve-to-external urethra sphincter reflex activity, which is essential for urine continence. In addition, blockage of spinal ERK 1/2 activation decreases the physiological function of the urethra, indicating that phosphorylation of the ERK 1/2 cascade may represent a novel target for the treatment of patients with neurological incontinence of spinal origin. (c) 2007 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/32990
ISSN: 0028-3908
文章連結: http://dx.doi.org/10.1016/j.neuropharm.2007.11.017
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