Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/33091
標題: Pulmonary toxicity of thuringiensin administered intratracheally in Sprague-Dawley rats
作者: Tsai, S.F.
廖俊旺
Liu, B.L.
Liao, J.W.
Wang, J.S.
Hwang, J.S.
Wang, S.C.
Tzeng, Y.M.
Ho, S.P.
何素鵬
關鍵字: thuringiensin
pulmonary toxicity
hydroxyproline
fibronectin
Bacillus
thuringiensis
aeruginosa exotoxin-a
performance liquid-chromatography
bronchoalveolar lavage fluid
bacillus-thuringiensis
extracellular-matrix
endothelial-cells
lung injury
bleomycin
fibrosis
mice
期刊/報告no:: Toxicology, Volume 186, Issue 3, Page(s) 205-216.
摘要: The purpose of this work is to evaluate the pulmonary toxicity of purified thuringiensin in Sprague-Dawley rats. Rats were intratracheally instillated with 0, 0.4, 0.8, 1.6, 3.2, 6.4 and 9.6 mg/kg body weight of thuringiensin. The results indicated that the acute pulmonary LD50 of thuringiensin for rats was 4.4 mg/kg. The total number of inflammatory cells and lactate dehydrogenase (LDH) activity in the bronchoalveolar lavage (BAL) fluid increased in a dose-dependent manner after thuringiensin instillation. Furthermore, an effective dose of 1.6 mg/kg was selected for the time course study of pulmonary toxicity. The treated animals showed a significant increase in the weights of the lungs, hydroxyproline levels in the lungs and total number of cells in BAL fluid 2, 4, 7, 14, 28 and 56 days after treatment. In comparison with the control, the total protein concentrations in BAL fluid were increased by 361, 615, 116, 41, 34 and 41%, after 2, 4, 7, 14, 28 and 56 days, respectively. The LDH activity in BAL fluid showed a significant increase after 1, 2, 4, 7, 14, 28 and 56 days. The increases in fibronectin levels were 164, 552, 490, 769, 335, 257 and 61% at the corresponding times, but neither tumor necrosis factor nor interleukin-1 increased. The treated rats presented abnormal histology including distributed inflammation in the bronchioles and alveoli, bronchial cellular necrosis on days I and 2, and areas of septal thickening with cellular infiltration and collagen deposit in the intestinal and alveolar spaces on days 4-56. Based on these biochemical and pathological parameters, intratracheal instillation of purified thuringiensin might cause significant pulmonary toxicity in rats. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/33091
ISSN: 0300-483X
文章連結: http://dx.doi.org/10.1016/s0300-483x(02)00744-8
Appears in Collections:獸醫病理生物學所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.