Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/33106
標題: Actinobacillus pleuropneumoniae serotype 10 derived ApxI induces apoptosis in porcine alveolar macrophages
作者: Chien, M.S.
宣詩玲
Chan, Y.Y.
Chen, Z.W.
Wu, C.M.
Liao, J.W.
Chen, T.H.
Lee, W.C.
Yeh, K.S.
Hsuan, S.L.
廖俊旺
簡茂盛
陳德勛
關鍵字: Actinobacillus pleuropneumoniae
ApxI
Porcine alveolar macrophages
Apoptosis
Caspase 3
cell-death
rtx toxins
kappa-b
lipopolysaccharide
pathways
activation
mechanisms
survival
protein
kinase
期刊/報告no:: Veterinary Microbiology, Volume 135, Issue 3-4, Page(s) 327-333.
摘要: Actinobacillus pleuropneumoniae (AP) is the causative agent of swine pleuropneumonia, a fibrinous, exudative, hemorrhagic, necrotizing pleuropneumonia affecting all ages of pigs. Actinobacillus pleuropneumoniae exotoxins (Apx) are one of the major virulence factors of AP. Due to the complex nature of Apx toxins produced by AP, little is known regarding the interactions of individual species of Apx toxin with target cells. The objective of this study was to examine whether AP serotype 10-derived exotoxin, ApxI, caused apoptosis in porcine alveolar macrophages (PAMs) and to delineate the underlying signaling pathways. Isolated PAMs were stimulated with different concentrations of native ApxI and monitored for apoptosis using Hoechst staining, TUNEL, and DNA laddering assays. The ApxI-stimulated PAMs exhibited typical morphological features of apoptosis, including condensation of chromatin, formation of apoptotic bodies and DNA laddering. ApxI-induced apoptosis in a concentration- and time-dependent manner. Furthermore, to delineate the signaling events involved in ApxI-induced apoptosis, it was observed that caspase 3 was activated in ApxI-stimulated PAMs. Ablation of caspase 3 activity via specific inhibitors protected PAMs from apoptosis by ApxI. This study is the first to demonstrate that native ApxI causes apoptosis in PAMs at low concentrations and that these apoptotic events are mediated via a caspase 3-dependent pathway. These findings suggest a role of ApxI in AP infection as it might impair the host defense system through the induction of apoptosis in PAMs. (C) 2008 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/33106
ISSN: 0378-1135
文章連結: http://dx.doi.org/10.1016/j.vetmic.2008.09.071
Appears in Collections:獸醫病理生物學所

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