Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/33148
標題: Ergothioneine protects against neuronal injury induced by cisplatin both in vitro and in vivo
作者: Song, T.Y.
廖俊旺
Chen, C.L.
Liao, J.W.
Ou, H.C.
Tsai, M.S.
關鍵字: Ergothioneine
Cisplatin
Avoidance tests
Neuron cells
Acetylcholinesterase activity
Oxidative stress
advanced ovarian-carcinoma
antioxidant system
alzheimers fibrils
diabetes-mellitus
oxidative damage
pc12 cells
acetylcholinesterase
melatonin
rats
nephrotoxicity
期刊/報告no:: Food and Chemical Toxicology, Volume 48, Issue 12, Page(s) 3492-3499.
摘要: The neuroprotective effects of ergothioneine (EGT) against cisplatin toxicity were investigated both in vitro and in vivo. For in vitro study, two types of neuronal cells, primary cortical neuron (PCN) cells and rat pheochromocytoma (PC12) cells, were incubated with EGT (0.1-10.0 mu M) for 2 h followed by incubation with 0.51 mu M cisplatin for 72 h. Results show that cisplatin markedly decreased the proliferation of PC12 cells and strongly inhibited the growth of axon and dendrite of PCN cells, but these effects were significantly prevented by EGT. For in vivo study, CBA mice were orally administered with 2 or 8 mg EGT/kg body weight for 58 consecutive days and were injected i.p. with 5 mg cisplatin/kg body weight on days 7, 9 and 11. We found that EGT significantly restored the learning and memory deficits in mice treated with cisplatin evaluated by active and passive avoidance tests. EGT also significantly prevented brain lipid peroxidation, restored acetylcholinesterase (AChE) activity and maintained glutathione/glutathione disulfide ratio in brain tissues of mice treated with cisplatin. These results demonstrate that EGT protects against cisplatin-induced neuronal injury and enhances cognition, possibly through the inhibition of oxidative stress and restoration of AChE activity in neuronal cells. (C) 2010 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/33148
ISSN: 0278-6915
文章連結: http://dx.doi.org/10.1016/j.fct.2010.09.030
Appears in Collections:獸醫病理生物學所

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