Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/33156
標題: Nanonized black soybean enhances immune response in senescence-accelerated mice
作者: Chan, Y.C.
廖俊旺
Wu, C.C.
Chan, K.C.
Lin, Y.G.
Liao, J.W.
Wang, M.F.
Chang, Y.H.
Jeng, K.C.
關鍵字: nanonized black soybean
cytokines
senescence accelerated mice
splenocytes
peripheral blood mononuclear cells
density-lipoprotein oxidation
age-associated decline
postmenopausal
women
soy isoflavones
gene-expression
in-vitro
t-cells
supplementation
protein
defects
期刊/報告no:: International Journal of Nanomedicine, Volume 4, Issue 1, Page(s) 27-35.
摘要: Soy isoflavones may have applications in cancer prevention and anti-inflammation, therefore this study was conducted to investigate the effect of dietary supplementation with black soybean on the immune response in the senescence-accelerated-prone mice (SAMP8) and-resistant mice (SAMPR1, as controls). The mechanism of isoflavones was also investigated. Six-month-old male SAMP8 and SAMR1 mice were divided into the control groups and experimental groups supplemented with nanonized (Nano-soy) or microparticled (Micro-soy) black soybeans (n = 8/group), respectively for 12 weeks. Human peripheral blood mononuclear cells (PBMC) and murine splenocytes were stimulated with mitogens and cytokines were determined by reverse transcriptase-polymerase chain reaction and/or ELISA. The results showed that body weight, food intake, and relative weights of organs did not differ among the SAMP8 control and experimental groups. Isoflavone (daidzin and genistin) intake was higher in the Nano-soy group than the Micro-soy group. The lymphoproliferation and production of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) in the Nano-soy group had a significantly higher (P < 0.05) than those in the control and Micro-soy groups. The Nano-soy supplemented mice reached these cytokine levels similar to SAMR1 mice. This result was consistent with the in vitro data that daidzein (a metabolite of daidzin), at a concentration of 10 mu M, increased IL-2, IL-4, and IFN-gamma production from phytohemagglutinin-stimulated PBMC (P < 0.05). However at higher concentrations (> 50 mu M), daidzein only reduced IL-10 and IFN-gamma levels, whereas genistein reduced levels of the IL-2, IL-4, IL-10, IFN-gamma mRNA and protein and these results suggest that the Nano-soy supplementation improved immune response in SAMP8 mice which may be attributable to higher daidzin content in the black soybean preparation.
URI: http://hdl.handle.net/11455/33156
ISSN: 1176-9114
Appears in Collections:獸醫病理生物學所

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