Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/33180
標題: Baculovirus-Derived Hemagglutinin Vaccine Protects Chickens from Lethal Homologous Virus H5N1 Challenge
作者: Lin, Y.J.
簡茂盛
Deng, M.C.
Wu, S.H.
Chen, Y.L.
Cheng, H.C.
Chang, C.Y.
Lee, M.S.
Chien, M.S.
Huang, C.C.
關鍵字: baculovirus expression system
H5N1
hemagglutination inhibition
highly
pathogenic avian influenza (HPAI)
subunit vaccine
avian influenza-viruses
reverse genetics
animals strategy
dna
vaccine
efficacy
evolution
infection
ducks
outbreak
subtypes
期刊/報告no:: Journal of Veterinary Medical Science, Volume 70, Issue 11, Page(s) 1147-1152.
摘要: Since outbreaks of highly pathogenic avian influenza (HPAI) in both human and poultry from 2003, it is critical to have effective vaccines. A cDNA fragment coding the entire hemagglutinin (HA) gene derived from an H5N1 strain (A/duck/China/E319-2/03) was cloned and expressed using the baculovirus system. Two weeks after receiving two doses of recombinant HA (rHA) vaccines, chickens develop high antibody response for hernagglutination inhibition (HI) at titer 7.2 log(2). Challenge studies revealed that vaccinated chickens with HI titers greater than 3 1092 Could have immunoprotection against the same HPAI H5N1 strain virus challenge through intranasal route. Additionally, HI titer of 5 log(2) determined whether the live viruses could not be detected from oropharyngeal, cloacal discharge or in tissues. This result suggests that the rHA expressed from baculovirus system could be a candidate for the development of a safe and efficient subunit vaccine for HPAI (H5N1).
URI: http://hdl.handle.net/11455/33180
ISSN: 0916-7250
文章連結: http://dx.doi.org/10.1292/jvms.70.1147
Appears in Collections:獸醫病理生物學所

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