Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/33184
標題: Virus antigen expression and alterations in peripheral blood mononuclear cell subpopulations after classical swine fever virus infection
作者: Lee, W.C.
簡茂盛
Wang, C.S.
Chien, M.S.
關鍵字: classical swine fever virus
PBMC phenotype
pig-viruses
hog-cholera virus
lymphocyte subpopulations
t-lymphocytes
pathogenesis
replication
cultures
pigs
modulation
apoptosis
phenotype
期刊/報告no:: Veterinary Microbiology, Volume 67, Issue 1, Page(s) 17-29.
摘要: Depletion in the number of lymphocytes and viral persistence are thought to be the most important outcomes of classical swine fever virus (CSFV) infection. To define the change in peripheral blood mononuclear cells (PBMC) and virus replication in leukocytes after CSFV infection, 8-week old pigs were infected with the LPC vaccine strain or virulent CSFV (HCV-YL strain). Changes in the relative number of PBMCs were analyzed by flow cytometry. The results showed a significant increase in the relative percentage of monocytes in PBMCs during acute CSFV infection of naive pigs (p < 0.05). Monocyte frequencies were not changed in LPC-vaccinated pigs and control pigs. There was also a significant decrease in the number of IgM(+) cells (p < 0.05) and a slight decrease in the number of CD4(+) lymphocytes after 5 days of infection. There was no change in the frequency of CD8(+) lymphocytes in PBMCs after infection. To define which subpopulation of PBMCs was the target for CSFV infection, PBMC populations from CSFV infected pigs were separated and stained for virus antigen expression. Alveolar macrophages (AM) were also studied. The results showed that CSFV replicated in all PBMC subpopulations: CD4(+), CD8(+), and IgM(+) lymphocytes, and monocytes as well as AMs. However, virus antigen expression was more intense in monocytes and AMs. The infection of lymphocytes may, therefore, contribute to the depletion in their numbers after infection and lead to defective antibody production during virulent CSFV infection. (C) 1999 Elsevier Science B.V. All rights reserved.
URI: http://hdl.handle.net/11455/33184
ISSN: 0378-1135
文章連結: http://dx.doi.org/10.1016/s0378-1135(99)00029-2
Appears in Collections:獸醫病理生物學所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.