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標題: Characterization of the Uptake and Intracellular Trafficking of G4 Polyamidoamine Dendrimers
作者: Pai, Chin-Ling
Shieh, Ming-Jium
Lou, Pei-Jen
Huang, Fei-Hong
Wang, Tzu-Wen
Lai, Ping-Shan
關鍵字: antisense oligonucleotides
transfection efficiency
pamam dendrimers
摘要: Polyamidoamine (PAMAM) dendrimers are highly branched spherical polymers that have emerged as potent synthetic drug and gene carriers; however, much remains to be learned about the mechanism of dendrimer-mediated cellular uptake. In this study, the endocytic pathway and intracellular trafficking of generation 4 (G4) PAMAM dendrimers were evaluated via fluorescein isothiocyanate (FITC) conjugation. We found that the G4-FITC dendrimers were internalized by energy-dependent and non-specific endocytic pathways. Interesting, G4-FITC dendrimers can not only buffer the endosomal/lysosomal pH but also co-localize with lysosomal markers over a period of 3 to 12 h, after which the signal decreased in the lysosomes and began to co-localize with the mitochondrial marker. This study contributes to the understanding of the molecular behaviour of G4 PAMAM dendrimers in a cellular environment and will facilitate the development of more effective PAMAM-mediated drug and gene delivery systems.
ISSN: 0004-9425
Appears in Collections:化學系所



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