Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/34007
標題: Continuous pressurized fluid extraction of Gynostemma pentaphyllum and purification of gypenosides
作者: Chen, C.H.
張傑明
Huang, T.Y.
Lee, M.R.
Hsu, S.L.
Chang, C.M.J.
李茂榮
關鍵字: dammarane-type glycosides
water extraction
carbon-dioxide
saponins
makino
期刊/報告no:: Industrial & Engineering Chemistry Research, Volume 46, Issue 24, Page(s) 8138-8143.
摘要: This work studied extractions of gypenosides from Gynostemma pentaphyllum using, batch and continuous pressurized fluids. Temperature was found to be a dominant factor in extracting gypenosides from this plant material. Using low solid to solvent ratio (50 g/L) was evidenced to avoid a rate limitation on batch pressurized water extraction of gypenosides. Within 3 h of continuous pressurized water extraction at 1.48 MPa, 373 K, 10 mL/min, a high solid to solvent ratio (200 g/L) resulted in 106.7 mg/g gypenosides. Our experimental results showed that 100% recovery of gypenosides, i.e., 164 mg/g, was obtained following pressurized 80% ethanol extraction at the same operation condition. Purification of the extract using Amberlite XAD7-HP column chromatography indicated that the purity of gypenosides increased from 24% to 83% by weight. In the bioassay aspect, the effect of concentrations of gypenosides in the extracts on the growth inhibition of two cancer cells was examined.
URI: http://hdl.handle.net/11455/34007
ISSN: 0888-5885
文章連結: http://dx.doi.org/10.1021/ie0705709
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