請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/34036
標題: Hot-pressurized fluid extraction of flavonoids and phenolic acids from Brazilian propolis and their cytotoxic assay in vitro
作者: Chen, C.R.
張傑明
Lee, Y.N.
Chang, C.M.J.
Lee, M.R.
Wei, I.C.
李茂榮
關鍵字: hot-pressurized fluids
water-soluble propolis extracts
flavonoids
phenolic acids
anti-oxidation ability
in vitro cytotoxicity
chemical-composition
artepillin-c
biological-properties
antioxidant
activity
phenethyl ester
tumor-cells
gc-ms
identification
constituents
toxicity
期刊/報告no:: Journal of the Chinese Institute of Chemical Engineers, Volume 38, Issue 3-4, Page(s) 191-196.
摘要: This work has examined the hot-pressurized fluid extraction of seven flavonoids, caffeic acid phenethyl ester and four phenolic acids from Brazilian propolis lumps generating, during the process, fat- and water-soluble extracts. The solid content of water-soluble extract obtained by hot-pressurized water in the presence of 29% natural surfactant was 35.2 mg/mL and was 44% greater than that obtained without natural surfactant. Furthermore the amount of the seven flavonoids and caffeic acid phenethyl ester in the fat-soluble extract exceeded those in the water-soluble sample while, on the other hand, the amount of the four phenolic acids in the water-soluble extract was more than those in the fat-soluble extract. Our findings show that the total solid content and the amount of these 12 active compounds produced by the emulsified hot-pressurized water are 36% and 7% higher, respectively, than those produced by emulsified water at atmospheric pressure. The EC50 value of the free radical scavenging activity of 1,1-diphenyl-2-picrythydrazyl of the emulsified hot-pressurized water extract was the lowest, and presented the strongest anti-oxidation ability among all the extracts. In vitro cytotoxicity indicated that the water-soluble extract strongly suppressed the growth of leukemia (HL-60, U937), lung cancer (A549, CH27) and liver cancer (Hep G2, Hep 3B) cells in a concentration-dependent behavior. (C) 2007 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/34036
ISSN: 0368-1653
文章連結: http://dx.doi.org/10.1016/j.jcice.2007.04.004
顯示於類別:化學系所

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