Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/34043
標題: Identification and evaluation of antioxidants defatted Camellia oleifera seeds by isopropanol salting-out pretreatment
作者: Chen, J.H.
張傑明
Wu, H.Y.
Liau, B.C.
Chang, C.M.J.
Jong, T.T.
Wu, L.C.
鍾婷婷
關鍵字: Camellia oleifera
Salting-out
TEAC
ORAC
ABTS*
fluorescence-based assay
kaempferol glycosides
free-radicals
tea
plant
flavonoids
sinensis
oligoglycosides
constituents
chemistry
analogs
期刊/報告no:: Food Chemistry, Volume 121, Issue 4, Page(s) 1246-1254.
摘要: Seven flavonoid glycosides (1-7) and one theasaponin (8) were isolated from the defatted Camellia oleifera seeds using the isopropanol salting-out pretreatment (ISP) technique, followed by column chromatography. Among them, compounds 5, 7, and 8 are new compounds. The comparison of oxygen radical absorbance capacity (ORAC) of crude extracts extracted by the n-butanol partition (BP) with those extracted by the ISP showed that the latter method yields eight times greater scavenging capacities than that of the former approach. Besides, ISP exhibited almost twice the extraction efficiency than that of BP. ORAC and Trolox equivalent antioxidant capacity (TEAC) analysis revealed that the most potent antioxidants are compounds 1 and 2, which exhibited 1.78 and 1.41 times higher antioxidant activity than that of Trolox, respectively. These results indicated that the ISP approach improves the extraction efficiency in the isolation of potent antioxidants, making the defatted C. oleifera seeds valuable in the food additive industry. (C) 2010 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/34043
ISSN: 0308-8146
文章連結: http://dx.doi.org/10.1016/j.foodchem.2010.01.015
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