Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3408
標題: The Optimization of Supercritical Carbon Dioxide and Near Critical Fluid Extractions of Artemisinin by Response Surface Methodology
超臨界二氧化碳與近臨界流體萃提青蒿素及其應答曲面最適化設計
作者: 林永龍
Lin, yung-lung
關鍵字: supercritical carbon dioxide
超臨界二氧化碳
N-hexane
Artemisia annua
artemisinin
response surface methodology
recovery
purity
正己烷共溶劑
應答曲面實驗設計
黃花蒿
青蒿素
回收率
純度
純化產量
出版社: 化學工程學系
摘要: This study utilized supercritical carbon dioxide and near critical carbon dioxide + N-hexane extractions of artemisinin from Artemisia annua by varying pressure (1000 psig to 5182 psig), temperature (30 C to 67 C), and co-solvent addition ratio (0 wt% to 22.56 wt%). The investigation probed into the total yield of the extracts, the recovery and the purity of artemisinin. Response surface methodology (RSM) was used to optimize extraction conditions for the recovery and the purity of artemisinin. Experimental results show that high pressure is favorable for the recovery and the purity of artemisinin at supercritical carbon dioxide extractions between 45 C and 50 C. Moreover, a pre-loading combined with continuing addition of co-solvent was proved to be effective in enhancing the recovery for near critical carbon dioxide + N-hexane extractions. Further experimental results of extracting 65 grams of Artemisia annua indicate that 648 grams of carbon dioxide added with 20 wt % of N-hexane at 2500 psig, 40 C, and 1.5 hours, produce 90.66 wt % of artemisinin recovery. However, near critical carbon dioxide + N-hexane extractions at high temperature (55 C) and high pressure (4500 psig) are definitely not helpful for the recovery and the purity. Finally, a design algorithm of 2000 psig and 40 C in a 2-factor and 3-level RSM discloses that carbon dioxide added with 16.25 wt % of N-hexane at 2700 psig, 33C, and 1.5 hour, can obtain an optimal value of the product of the recovery and the purity of artemisinin
本研究以改變壓力(1000 psig至5182 psig)、溫度(30 °C至67 °C) 、與共溶劑添加比例(0 wt% 至22.56 wt%)的超臨界流體萃取技術,探討黃花蒿萃出物中青蒿素含量及其純度的變化情形。並利用應答曲面實驗設計法(RSM),分別搜尋且獲得青蒿素回收率、純度、與純化產量(前兩者乘積)的最適萃取條件。研究中發現,在45°C至50°C,高壓有利於超臨界二氧化碳的萃取製程。然後,由添加正己烷的近臨界二氧化碳萃取製程瞭解,預添加合併連續添加的方式最有利於青蒿素的回收率。數據顯示,添加20 wt % 正己烷的648 克二氧化碳,在2500 psig,40 °C,1.5小時的近臨界態萃取65克黃花蒿,獲得90.66 wt%的青蒿素回收率。並發現較高溫度(55°C)與較高壓力(4500 psig)的近臨界萃取製程,不利於回收率與純度。最後,採用2000 psig與40 °C二因子三階層中心混成,做為RSM的設計點。並經由實驗數據的應答曲面極大值,預知添加16.25 wt%正己烷的648克二氧化碳,在2700 psig,33 °C,1.5小時的近臨界態萃取,可得到最佳的青蒿素純化產量。
URI: http://hdl.handle.net/11455/3408
Appears in Collections:化學工程學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.