Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/34188
標題: Trace determination of sulfonamides residues in meat with a combination of solid-phase microextraction and liquid chromatography-mass spectrometry
作者: Lu, K.H.
李茂榮
Chen, C.Y.
Lee, M.R.
關鍵字: solid-phase microextraction
meat
sulfonamides
liquid
chromatography-mass spectrometry
fluorescence detection
postcolumn derivatization
drug residues
quantitative-determination
dispersion extraction
milk
quantification
urine
water
antibiotics
期刊/報告no:: Talanta, Volume 72, Issue 3, Page(s) 1082-1087.
摘要: An integrated method of combining solid-phase microextraction (SPME) with liquid chromatography-mass spectrometry (LC-MS) was evaluated for determination trace amount of sulfonamides in meat products. Eight commonly used sulfonamides, sulfadiazine (SDZ), sulfathiazole (STZ), sulfamerazine (SMR), sulfamethazine (SMT), sulfamonomethoxine (SMMX), sulfamethoxazole (SMXZ), sulfaquinoxaline (SQX) and sulfadimethoxine (SDMX), were investigated in this study. Chromatography was performed on a C(18) reversed-phase column using an isocratic, acetonitrile in water as the mobile phase. Fiber coated with a 65 mu m thickness of polydimethylsiloxane/divinylbenzene (PDMS/DVB) was used to extract sulfonamides at optimum conditions. Analytes were desorbed with static desorption in an SPME-HPLC desorbed chamber for 15 min and then determined by LC-MS. The detection limits of these sulfonamides in pork were from 16 mu g kg(-1) (SMT) to 39 mu g kg-1 (SMMX). According to the analysis, the linear range was from 50 to 2000 mu g kg(-1) with relative standard deviation (R.S.D.s) value below 15% (intra-day) and 19% (inter-day). The proposed method was tested by analyzing meats. from a local market for sulfonamides residues. Some sulfonamides in our study were detected in the meat samples. The concentration of these residual sulfonamides ranged from 66 mu g kg-1 (SDZ) to 157 mu g kg(-1) (SQX) in a chicken sample. The results demonstrate that the SPME-LC-MS system is highly effective in analyzing trace sulfonamides in meat products. (C) 2007 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/34188
ISSN: 0039-9140
文章連結: http://dx.doi.org/10.1016/j.talanta.2007.01.022
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