請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/34219
標題: Engineering lysine-rich caleosins as carrier proteins to render biotin as a hapten on artificial oil bodies for antibody production
作者: Liu, T.H.
李豐穎
Chyan, C.L.
Li, F.Y.
Chen, Y.J.
Tzen, J.T.C.
關鍵字: antibody production
artificial oil bodies
biotin
caleosin
hapten
linked-immunosorbent-assay
enzyme-immunoassay
escherichia-coli
purification
surface
expression
system
elisa
cell
期刊/報告no:: Biotechnology Progress, Volume 27, Issue 6, Page(s) 1760-1767.
摘要: It has been demonstrated that caleosin alone is sufficient to stabilize artificial oil bodies. A series of recombinant caleosins, mutated with 3, 5, 8, 11, 13, 15, and 17 extra Lys residues and over-expressed in Escherichia coli, were used as carrier proteins to render biotin as a hapten on the surface of artificial oil bodies for antibody production. Biotinylation levels of the recombinant caleosins were step-wisely elevated as the number of extra Lys residues increased, and the biotinylated Lys residues were identified by mass spectrometric analysis. Polyclonal antibodies against biotin were successfully generated in rats injected with artificial oil bodies constituted with each of the biotinylated caleosins. Moreover, those generated via the biotinylated caleosins with eight or more extra Lys residues no longer recognized caleosin. It appears that engineered Lys-rich caleosins are suitable carrier proteins for the production of antibodies against small molecules. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011
URI: http://hdl.handle.net/11455/34219
ISSN: 8756-7938
文章連結: http://dx.doi.org/10.1002/btpr.684
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