Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/34625
標題: The interleukin-4 expression in patients with severe sepsis
作者: Wu, H.P.
莊敦堯
Wu, C.L.
Chen, C.K.
Chung, K.
Tseng, J.C.
Liu, Y.C.
Chuang, D.Y.
關鍵字: Interleukin-4
Interleukin-4 delta 2
Sepsis
Cytokines
Human
alternative splice variant
cell-proliferation
human il-4
il-4-delta-2
tuberculosis
gamma
期刊/報告no:: Journal of Critical Care, Volume 23, Issue 4, Page(s) 519-524.
摘要: Purpose: Sepsis is a complicated syndrome in which proinflammatory and anti-inflammatory cytokines are expressed simultaneously. However, it remains unclear for the expression of interleukin (IL)-4 and IL-4 delta 2 in patients with severe sepsis. Materials and Methods: By nested reverse transcriptase-polymerase chain reaction and the expression of glyceraldehydes-3-phosphate dehydrogenase as the internal reference, the expression levels of IL-4 and IL-4 delta 2 were determined in peripheral blood mononuclear cells (PBMCs) of 76 patients with severe sepsis and were immediately admitted to an intensive care unit. Plasma IL-4 level was measured by enzyme-linked immunosorbent assay. Clinical characteristics were monitored and recorded prospectively. Results: The IL-4 messenger RNA (mRNA) expression in PBMCs of patients who had survived was significantly higher than that of those who had died. The IL-4 delta 2/IL-4 ratio in PBMCs of patients who had survived was significantly lower than that of those who had died. The IL-4 delta 2 expression did not differ between survivors and nonsurvivors. After regression analysis, the IL-4 delta 2/IL-4 ratio still was all independent factor for death in patients with severe sepsis. The expression of IL-4 delta 2 mRNA was positively correlated with that of IL-4 mRNA in patients with severe sepsis. The plasma IL-4 levels ill septic patients oil admission day did not differ between survivors and nonsurvivors. Conclusions: The IL-4 mRNA expression might be associated with survival in patients with severe sepsis. The IL-4 delta 2/IL-4 ratio might be served as the net immunity of IL-4 activity. (c) 2008 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/34625
ISSN: 0883-9441
文章連結: http://dx.doi.org/10.1016/j.jcrc.2007.11.008
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