Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/34881
標題: An enzymeless electrochemical sensor for the selective determination of creatinine in human urine
作者: Chen, J.C.
曾志明
Kumar, A.S.
Chung, H.H.
Chien, S.H.
Kuo, M.C.
Zen, J.M.
關鍵字: creatinine
human urine
screen-printed electrode
preanodization
glassy-carbon electrodes
voltammetric determination
liquid-chromatography
serum creatinine
performance
biosensor
acid
microstructure
activation
impedance
期刊/報告no:: Sensors and Actuators B-Chemical, Volume 115, Issue 1, Page(s) 473-480.
摘要: Determination of creatinine in various biological fluids is useful for evaluation of renal, muscular and thyroid dysfunctions. An enzymeless electrochemical approach for the selective and quantitative recognition of creatinine in human urine has been demonstrated by using a preanodized screen-printed carbon electrode (SPE*). During a preconcentration step (at 1.8 V versus Ag/AgCl), the formation of a stable carbon-carbon bond between the electro-generated > C=O of SPE* and the active methylene group of creatinine was identified by XPS. By using the SPE* together with a medium exchange procedure, the creatinine was selectively detected in the window of 0.37-3.6 mM with a slope and regression coefficient of 16.7 mu A/mM and 0.998, respectively, by square-wave voltammetry. Ten successive detection of 0.37 mM creatinine showed a relative standard deviation of 3.4%. indicating a detection limit (signal/noise = 3) of 8.6 mu M. Real human urine samples were analyzed by this method and compared with the results obtained from the Jaffe reaction procedure. (c) 2005 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/34881
ISSN: 0925-4005
文章連結: http://dx.doi.org/10.1016/j.snb.2005.10.015
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