請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/34929
標題: alpha-Cyclodextrin-modified infrared chemical sensor for selective determination of tyrosine in biological fluids
作者: Lee, C.J.
楊吉斯
Yang, J.
關鍵字: FT-IR
IR sensor
evanescent wave
alpha-cyclodextrin
amino acids
tyrosine
performance liquid-chromatography
mass-spectrometry
chlorinated
hydrocarbons
amino-acids
phenylalanine
plasma
blood
water
tryptophan
histidine
期刊/報告no:: Analytical Biochemistry, Volume 359, Issue 1, Page(s) 124-131.
摘要: In this paper, we propose an evanescent wave-based infrared (IR) spectroscopic sensing method for the selective and sensitive detection of tyrosine in aqueous solution. In this approach, alpha-cyclodextrin (alpha-CTD) was chemically immobilized onto the surface of an IR-sensing element to attract tyrosine specifically to the surface of the sensing element. Theoretical equations were developed for the quantitative analysis of tyrosine. Based on its IR spectra, the synthesized alpha-CTD phase was stable in water. Optimal detection with this system occurred when the pH of the solution was ca. 10.5. Based on the absorption bands, we confirmed that alpha-CTD was most effective at attracting tyrosine under basic conditions. Using the unique absorption band of tyrosine at 1500 cm(-1), the alpha-CTD phase allowed the detection of tyrosine selectively from among a range of potentially interfering amino acids and other species commonly present in biological samples. For quantitative analysis, this CTD-modified phase was most suitable for sensing tyrosine at concentrations below 100 mu M because of limits in the surface adsorption mechanism. The detection times were, in some instances, lower than 5 min. For a detection time of 10 min, the detection limit of tyrosine was ca. 0.4 mu M. (c) 2006 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/34929
ISSN: 0003-2697
文章連結: http://dx.doi.org/10.1016/j.ab.2006.09.004
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