Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/35128
標題: Magnesium lithospermate B possesses inhibitory activity on Na+,K+-ATPase and neuroprotective effects against ischemic stroke
作者: Tzen, J.T.C.
李豐穎
Jinn, T.R.
Chen, Y.C.
Li, F.Y.
Cheng, F.C.
Shi, L.S.
She, H.K.H.
Chen, B.C.M.
Hsieh, V.
Tu, M.L.
曾志正
關鍵字: cardiac glycosides
magnesium lithospermate B
Na+,K+-ATPase
neuroprotection
ouabain
Salvia miltiorrhiza
salvia-miltiorrhiza
derivatives
receptor
discovery
failure
rats
期刊/報告no:: Acta Pharmacologica Sinica, Volume 28, Issue 5, Page(s) 609-615.
摘要: Aim: To examine if magnesium lithospermate B (MLB) extracted from Danshen, the dried roots of Salvia miltiorrhiza, may act as an active component responsible for the cardiac therapeutic effect of this traditional Chinese herb via the same molecular mechanism triggered by cardiac glycosides, such as ouabain and digoxin. Moreover, we wanted to test if MLB may provide neuroprotection against ischemic stroke as observed for cardiac glycosides. Methods: Similarity in the chemical structure and molecular configuration between MLB and ouabain was analyzed. The inhibition potency of MLB and ouabain on Na+,K+-ATPase activity of a commercial product, as well as in purified membrane fractions from rat brain and heart tissues, was examined and compared. Neuroprotective effect of MLB against ischemic stroke was also evaluated using a cortical brain slice-based assay model. Results: Dose-dependent inhibition on the commercial Na+,K+-ATPase equivalent to that for ouabain was observed for MLB of approximately half dosage by weight. This relative potency of ouabain and MLB was also observed for their inhibition on Na+,K+-ATPase activity of plasma membrane purified from rat tissues, although these 2 inhibitors exhibited somewhat lower competence in these crude extracts. In ischemic gerbil brains, post-treatment with MLB significantly reduced the infarct size, visualized by 2,3,5-triphenyltetrazolium chloride staining, by approximately 55% when compared with the control group. Conclusion: These results evidently suggest that the cardiac therapeutic effect of Danshen should be at least partly attributed to the effective inhibition of Na+,K+- ATPase by MLB, and that MLB provides anti-ischemic neuroprotection in gerbils subjected to focal ischemia and reperfusion.
URI: http://hdl.handle.net/11455/35128
ISSN: 1671-4083
文章連結: http://dx.doi.org/10.1111/j.1745-7254.2007.00544.x
Appears in Collections:化學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.