請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/35129
標題: Gene families encoding isoforms of two major sesame seed storage proteins, 11S globulin and 2S albumin
作者: Hsiao, E.S.L.
李豐穎
Lin, L.J.
Li, F.Y.
Wang, M.M.C.
Liao, M.Y.
Tzen, J.T.C.
曾志正
關鍵字: 11S globulin
2S albumin
MALDI-MS
seed storage protein
sesame
mass-spectrometry
alpha-globulin
indicum l
identification
allergy
children
pattern
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 54, Issue 25, Page(s) 9544-9550.
摘要: Sesame (Sesamum indicum L.) seed has been recognized as a nutritional protein source owing to its richness in methionine. Storage proteins have been implicated in allergenic responses to sesame consumption. Two abundant storage proteins, 11S globulin and 2S albumin, constitute 60-70 and 15-25% of total sesame proteins, respectively. Two gene families separately encoding four 11S globulin and three 2S albumin isoforms were identified in a database search of 3328 expresssed sequence tag (EST) sequences from maturing sesame seeds. Full-length cDNA sequences derived from these two gene families were completed by PCR using a maturing sesame cDNA library as the template. The amino acid compositions of these deduced storage proteins revealed that the richness in methionine is attributed mainly to two 2S albumin isoforms and partly to one 11S globulin isoform. The presence of four 11S globulin and three 2S albumin isoforms resolved in SDS-PAGE was confirmed by MALDI-MS analyses. The abundance of these isoforms was in accord with the occurrence frequency of their EST sequences in the database. A comprehensive understanding of these storage proteins at the molecular level may also facilitate the identification of allergens in crude sesame products that have caused severe allergic reactions increasingly reported in the past decade.
URI: http://hdl.handle.net/11455/35129
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf061505x
顯示於類別:化學系所

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。