請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/35130
標題: Stability of Artificial Oil Bodies constituted with Recombinant Caleosins
作者: Liu, T.H.
李豐穎
Chyan, C.L.
Li, F.Y.
Tzen, J.T.C.
曾志正
關鍵字: Artificial oil bodies
caleosin
oleosin
structural stability
thermostability
escherichia-coli
structural-properties
oleosin
purification
proteins
calcium
surface
system
biogenesis
expression
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 57, Issue 6, Page(s) 2308-2313.
摘要: Caleosin is a unique calcium binding protein anchoring to the surface of seed oil bodies by its central hydrophobic domain composed of an amphiphatic alpha-helix and a proline-knot subdomain. Stable artificial oil bodies were successfully constituted with recombinant caleosin overexpressed in Escherichia coli. The stability of artificial oil bodies was slightly or severely reduced when the amphiphatic alpha-helix or proline-knot subdomain in the hydrophobic domain of caleosin was truncated. Deletion of the entire central hydrophobic domain substantially increased the solubility of the recombinant caleosin, leading to a complete loss of its capability to stabilize these oil bodies. A recombinant protein engineered with the hydrophobic domain of caleosin replaced by that of oleosin, the abundant structural protein of seed oil bodies, could stabilize the artificial oil bodies, in terms of thermo- and structural stability, as effectively as caleosin or oleosin.
URI: http://hdl.handle.net/11455/35130
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf803566w
顯示於類別:化學系所

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。