Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/35149
標題: Multiple screening of urolithic organic acids with copper nanoparticle-plated electrode: Potential assessment of urolithic risks
作者: Yang, C.W.
曾志明
Zen, J.M.
Kao, Y.L.
Hsu, C.T.
Chung, T.C.
Chang, C.C.
Chou, C.C.
張照勤
周濟眾
張登欽
關鍵字: Urolithiasis
Creatinine
Organic acids
Electrochemical
Nanoparticle-plated electrode
performance liquid-chromatography
uric-acid
capillary-electrophoresis
electrochemical detection
calcium-oxalate
canine uroliths
stone
formers
renal-disease
nephrolithiasis
creatinine
期刊/報告no:: Analytical Biochemistry, Volume 395, Issue 2, Page(s) 224-230.
摘要: There is yet to be a reliable prediction of urolithiasis. To facilitate early diagnosis, a simple and rapid high performance liquid chromatography method with electrochemical detection using disposable copper-nanoparticle-plated electrodes (Cu(n)-SPE) was developed for multiple detection of creatinine and 4 urolithic organic acids. A total of 206 normal and urolithic human and canine urines and urolith samples were collected for direct analysis of creatinine, cystine, uric acid, oxalic acid, and citric acid without sample cleanup and derivatization processes. Urinary organic acids were separated in 11 min and were devoid of ascorbic acid interference. The detection limits (S/N > 3) were at the nanomolar level with linear dynamic ranges spanning 2-3 orders of magnitude. Recoveries in urine ranged from 99.5% for creatinine to 86.5% for citric acid. The analytical variations (RSD) were less than 6.2% in phosphate buffer and 7.7% in urine. Important differences in organic acid levels/profiles between animal species and among normal and urolithic urines/urolith were unveiled and corresponded well (70-90%) with the urolithic risk in a retrospective assessment. The simplicity and reproducibility of this method using disposable Cu(n)-SPE has made routine urine analysis possible and can be of great clinical and diagnostic potential in the screening of urolithiasis and abnormal states related to excess secretion of organic acids and amino acids in humans and animals. (C) 2009 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/35149
ISSN: 0003-2697
文章連結: http://dx.doi.org/10.1016/j.ab.2009.08.020
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