Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/35152
標題: Direct determination of sinigrin in mustard seed without desulfatation by reversed-phase ion-pair liquid chromatography
作者: Jen, J.F.
黃振文
Lin, T.H.
Huang, J.W.
Chung, W.C.
鄭政峰
關鍵字: mustard seed
food analysis
sinigrin
glucosinolates
carbohydrates
electrokinetic capillary chromatography
products
isothiocyanates
期刊/報告no:: Journal of Chromatography A, Volume 912, Issue 2, Page(s) 363-368.
摘要: Reversed-phase ion-pair liquid chromatography has been investigated for directly analyzing sinigrin in mustard seed without desulfatation. After extraction by phosphate buffer (pH 7.0) from the grind-pastes of inactivated-myrosinase mustard seeds, sinigrin was first isolated through deproteinization and centrifugation, followed by filtration and injection into the chromatographic system. A reversed-phase C-18 column was used to separate the sinigrin with an eluent of acetonitrile (ACN)-water (20:80) containing 0.02 M tetrabutylammonium (TBA) as the counter ion at pH 7.0. Detection was carried out with an UV detector operated at 227 nm. Factors affecting the chromatographic separation and quantitative determination, such as concentrations of TEA and ACN, and pH, were studied. The linear dynamic range is larger than three orders of magnitude and the detection limit is 0.045 mg/L. The RSD is around 3% and the recovery is 85% (3% RSD, n = 3). (C) 2001 Published by Elsevier Science B.V.
URI: http://hdl.handle.net/11455/35152
ISSN: 0021-9673
文章連結: http://dx.doi.org/10.1016/s0021-9673(01)00591-x
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