Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/35416
標題: 輪蟲循環水養殖系統之研發
Development of the Recirculating Culture System for Rotifer
作者: 劉俊宏
Liu, Chun-Hung
關鍵字: Rotifer
輪蟲
Recirculation system
Semi-continuous culture
循環水系統
半連續培養
出版社: 生物產業機電工程學系所
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摘要: 本研究旨在設計開發一套循環水輪蟲養殖系統,藉以生產極小型(SS)輪蟲;系統主要由生物濾床、試驗養殖槽、微藻添加槽、微藻儲存槽、控溫系統與自動進排液系統等裝置所建構而成。研究首先選取四種餌料大溪地等鞭金藻、麵包酵母、綠藻粉與育苗乳進行試驗,以大溪地等鞭金藻獲致輪蟲之增殖密度最高;而進一步進行相關批次基礎試驗,其中包括輪蟲接種密度、大溪地等鞭金藻餌料密度、添加藻水方式與溫度等試驗,進而獲致批次試驗時以接種密度50 ind./ml、大溪地等鞭金藻密度100×104 cells/ml、添加一次藻水與溫度35℃條件下為極小型(SS)輪蟲最適之培養條件;本研究亦進行批次放大培養12與60公升試驗,獲致12 公升培養試驗以未曝氣方式較佳,而60 公升培養試驗則以降低波動程度投餵大溪地等鞭金藻餌料,使輪蟲受驚嚇之情形減緩,可獲致輪蟲密度最高約1216 ind./ml;進一步以半連續循環水系統,在設定水力停留時間(HRT) 2小時、稀釋率16.67% (10/60)下進行兩次培養試驗,第一次半連續試驗時,其批次放大培養為6天,半連續培養為18天,結果獲致每日平均收穫密度約827 ind./ml極小型(SS)型輪蟲10公升,同時生物濾床之硝化作用可將銨濃度從1.6 mg/l降至0.005 mg/l以下,其亞硝酸根離子濃度則可從5 mg/l降至0.5 mg/l;第二次半連續培養試驗,則以計時器定時控制進料與出料之電磁閥,使系統在自動化半連續操作下進行輪蟲培養,試驗時亦先批次放大培養3天,經半連續培養23天之測試,結果獲致每日平均收穫密度約1099 ind./ml之極小型輪蟲10公升,且培養期間系統均可維持輪蟲密度介於915-1450 ind./ml之間;同時獲致培養26天之平均比增殖率為0.182 day-1,因此證實本研究之自動化半連續循環水系統,已具有良好且穩定培養輪蟲之能力。
A rotifer's recirculation culture system which could be used to rear the rotifer Brachoinus sp. was designed and developed in this research. The system was constructed and combined together with the parts of the bio-filter tank, the culture tank, the algal supply tank, the algal storage tank, the temperature control system, and the auto cultivating solution inlet and outlet control equipment. The four varieties of feeds, the alga Isochrysis aff. galbana, the baker yeast, the freshwater Chlorella powder and the PUFA emulsion were first selected as rotifer's food for the batch culture test. The test with the alga Isochrysis aff. galbana held the highest growth density. The rotifer inoculated density, cell density of the alga Isochrysis aff. galbana, the feeding method and the temperature of the cultivating conditions to influence the rotifer growth were also studied. The experimental results of the fundamental batch tests showed that the optimal rotifer inoculated density, cell density of the alga Isochrysis aff. galbana, the feeding method and the temperature were 50 ind./ml, 100×104 cells/ml, one-supply and 35℃, respectively. The batch raising cultivation of rotifer with 12 and 60 liters were also conducted. The method of non-aeration was the more appropriate way obtained in 12 liters test. The 60 liters test could get the maximum rotifer growth density 1216 ind./ml as the feeding shock reduced. Two semi-continuous cultivating tests using the newly developed recirculating-water culture system with the hydraulic retention time (HRT) two hours and the daily dilution ratio 16.67%(10/60) were performed. The first experiment with six days of batch raising cultivation and eighteen days of semi-continuous cultivation could produce the harvested solution 10 liters with the daily average rotifer harvesting density 827 ind./ml. It also demonstrated that the bio-filter held the nitrification function and the concentration of NH4+ was dropped from 1.6 mg/l to 0.005 mg/l with NO2- reduced from 5 mg/l to 0.5 mg/l. The second experiment used timer to control the cultivating solution inlet and outlet electromagnetic valves and resulted in the automatic semi-continuous culture of the rotifer. The test was first with three days of batch raising cultivation and followed with twenty-three days of semi-continuous cultivation. The experimental result showed that the system could steadily produce 10 liters of daily harvested solution as the daily average rotifer harvesting density of 1099 ind./ml. During the cultivating period, the rotifer growth density could be maintained between 915 and 1450 ind./ml with the average growth rate 0.182 day-1. The experiment also verified that the automatic semi-continuous recirculating-water culture system presently developed in this research obtained the capability of stable and fine cultivating functions to produce rotifers.
URI: http://hdl.handle.net/11455/35416
其他識別: U0005-2708200617510100
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2708200617510100
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