Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3557
標題: 以二維擴散模型模擬溶質分子在均相中之質傳模式
Research on the Mass Transfer Mechanism of Solutes in Homogeneous Phase with a Two-Dimensional Diffusion Model
作者: 鄭培琳
Jeng, Pei-Lin
關鍵字: Cytoplasmic transport
細胞質
diffusion
simulates molecular diffusion
分子擴散
模擬
出版社: 化學工程學系所
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摘要: 細胞質不僅是細胞內生物化學反應的場所,也是維持細胞運作的重要媒介,例如蛋白質之合成,DNA在細胞核內轉錄(Transcription)成mRNA,mRNA由細胞核中被運送出來至細胞質內再與細胞質內之核醣體或是粗糙內質網(Rough ER)上之核醣體進行轉譯(Translation)合成蛋白質。細胞質含有豐富水相的細胞質液(Cytosol)以及多種蛋白質、細胞骨骼纖維絲(Cytoskeleton Filaments)、有機分子、無機分子及酵素等的細胞基質(Cytoplasmic Matrix)。細胞必需依靠養分、代謝反應及訊息傳遞來調節維持細胞內外環境,不僅在細胞與細胞、細胞與外界環境之間物質的交換與輸送,或是細胞內部的調控,細胞質的輸送(Cytoplasmic Transport)對於調節細胞內外生理環境來說是不可或缺的一個環節。然而分子在細胞內的輸送包括有主動運輸以及被動運輸兩項機制。細胞內的一些結合蛋白攜帶一部分小分子溶解於細胞質內進行其他生物化學反應、 小囊泡藉由驅動蛋白(Dynein)之驅動力攜帶生物分子沿著細胞骨骼絲(Cytoskeleton)進行運輸及分子與細胞膜內表面結合進行橫向運輸(Lateral Diffusion)等方式屬於主動運輸。被動運輸則包括了分子因濃度差,以細胞質液為介質往濃度低的地方進行擴散作用。 本研究利用小鼠卵細胞及M2培養液進行螢光分子顯微注加實驗,並以二維數學模型模擬螢光分子在均相溶液中進行擴散作用之情形,二者相互比較並且針對生物分子在細胞質內的輸送計算出擴散係數之值。綜合實驗及模擬發現不同濃度之螢光分子在相同條件下進行擴散作用,不同濃度的螢光分子除了可計算出擴散係數之外,亦可估計出達到擴散均勻的時間。 由本研究可知此Fortran程式模型已經可以合理模擬分子擴散,未來除了可以使用適合的動物細胞來進行實驗之外,並可發展出3-D模型,進而再一步步修正理論使其更趨近真實狀況。
Cytoplasm serves not only as the reactor of the biochemical processes in the cell, but also the important medium for the housekeeping, which is critical in maintaining the homeostasis within the cell. Examples include synthesis of proteins and transport of mRNA from nucleus via cytoplasm to rough ER to complete translation. Cytoplasm is thought to be composed of cytosol in aqueous phase, a variety of protein, cytoskeleton filaments, and cytoplasmic matrix, which in turn comprises organic/inorganic molecules and enzymes. It requires nutrients, metabolism and intracellular communication all functioning properly to keep the interior and exterior of a living cell at desired conditions. Thus it is apparent that the cytoplasmic transport plays an extremely important role in the regulation of the physiological conditions inside and outside the cell. Cytoplasmic transport is categorized into active and passive transport. Dynein, as a molecular motor, carrying biomolecules and moving along cytoskeleton is exemplified as active transport. Whereas passive transport essentially arises from the concentration gradient across the cytosol. In this study, fluorescent microinjection into mouse oocytes and M2 medium is employed to study the diffusion processes, in addition, a two-dimensional mathematical model quantifies the experimental results. Coupled results from experiments and model enable the determination of the diffusion coefficients of various medium, as well as the time length required to achieve steady states. The model simulates molecular diffusion to reasonable degree and flourishes the succeeding research on intracellular communication including both active and passive transport.
URI: http://hdl.handle.net/11455/3557
其他識別: U0005-0608200607494000
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