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標題: 低強度超音波與低功率雷射對內皮細胞活性之影響
The effects low-intensity ultrasound and low-energy laser on the functions of endothelial cells
作者: 王冠卜
Wang, Kuan-Pu
關鍵字: low-intensity ultrasound
endothelial cells
出版社: 化學工程學系所
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摘要: 低強度超音波刺激在骨科修復、癌症治療與非侵入式醫學影像,至目前已被研究探討達50多年,雖有研究報導指出對於細胞生理效應有正面影響,但對於遠場超音波刺激內皮細胞的影響至今很少有文獻探討之。本研究利用遠場低強度超音波以不同刺激強度(0.5、1.0及1.5 W/cm²)刺激人類靜脈內皮細胞(HUVECs),觀察內皮細胞增生與NO釋放,並以eNOS基因與蛋白的表現與其可能的分子調控機制。研究結果指出,低強度超音波以刺激強度為1.0 W/cm²連續刺激3天,對於HUVECs細胞有抑制增生但對於其NO的釋放有一正面效果。於RT-PCR與西方墨點法的結果顯示,同樣低強度超音波以刺激強度為1.0 W/cm² 連續刺激3天對於內皮細胞eNOS的基因或蛋白上皆有提高其表現。此外,低強度超音波以刺激強度為1.0 W/cm²刺激1天並利用H89抑制PKA的活性對於內皮細胞eNOS蛋白磷酸化較具有抑制的效果,而對Akt則無明顯差異,但低強度超音波刺激內皮細胞所引發的eNOS活性的訊息傳遞則需更進一步探討研究之。低強度超音波與低功率二極體雷射刺激牛頸動脈內皮細胞,結果顯示雷射刺激對於內皮細胞的影響有較快的回應,但較無維持的效果;反之,超音波刺激對於內皮細胞的影響需要較長時間予以回應,卻可以維持較長時間的效果。
Ultrasound was transmitted through and into biological tissues as an acoustic pressure wave. Ultrasound was used widely in medicine as a therapeutic, operative, and diagnostic tool. Low-intensity ultrasound was used to accelerate fracture healing in the 1950s. The effects of low-intesity ultrasound stimulation on human umbilical vein endothelial cells (HUVECs) were investigated in this study. Low-intensity ultrasound was used to stimulate HUVECs, and its effects on cell proliferation, nitric oxide (NO) secretion, and protein expression were determined. From the study, the possible molecular mechanism of low-intensity ultrasound stimulation on endothelial cells was proposed. Exposure to 1 MHz pulsed wave ultrasound at 1.0 W/cm² for 10 min once daily for three days increased nitric oxide secretion and enhanced endothelial nitric oxide synthase (eNOS) activity. The proliferation of HUVECs did not change much when exposed to the low-intensity ultrasound. The enhancing effect on cell growth was not apparent for HUVECs. The induced phosphorylation of eNOS (Ser1177) by low-intensity ultrasound at 1.0 W/cm² for 10 min was inhibited by addition of N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinoline sulfonamide (H89, protein kinase A inhibitor). The inhibition by 2-(4-Morpholino)-8-phenyl-4H-1-benzopyran-4-one (LY294002, phosphoatidylinositol 3-kinase inhibitor) was not significant. The induced phosphorylation of Akt (Ser473) by low-intensity ultrasound at 1.0 W/cm² for 10 min was inhibited by addition of LY294002. The inhibition in the present of H89 was not significant. Our results provided indirect evidence and supported that low-intensity ultrasound promotes eNOS activity via the PI3K/Akt/PAK signaling pathway.Low-energy laser induced bovine carotid artery endothelial cells (BECs) in NO secretion and eNOS gene expression, which was a faster response. Low-intensity ultrasound induced BECs in NO secretion and eNOS gene expression at longer terms but the effect also remain for longer period of time.
其他識別: U0005-0908200611554500
Appears in Collections:化學工程學系所



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