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標題: 可同時量測細胞呼吸活性與酸化率之細胞晶片的研發
A cell-based chip for the simultaneous measurement of cellular respiratory activity and acidification rate
作者: 林孝安
Lin, Shiau-An
關鍵字: 氧化銥
Iridium oxide
cellular respiration
acidification rate
salt bridge microchannel
cell-based chip
出版社: 生物產業機電工程學系所
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摘要: 近幾年來結合微製程製作的細胞感測器,已廣泛受到重視,藉由量測細胞外溶氧濃度與pH值的變化,可得知細胞呼吸活性與酸化率的狀態。研究中藉由微製程技術與電沈積技術製作非Clark-type溶氧電極與IrOx-pH電極,分別被用以量測細胞呼吸活性與酸化率。在0.6~1.0 mA/cm2範圍內電流密度(current density, CD)所沈積的IrOx電極其靈敏度與厚度並無顯著差異。比較以0.8 mA/cm2沈積五分鐘(5M/0.8CD-),十分鐘(10M/0.8CD-)與15分鐘沈積(15M/0.8CD-deposited) IrOx-pH電極的90 % (t90)反應時間,分別為2.2 ± 0.9 s、 7.1 ± 0.7 s與12.8 ± 1.6 s,顯示膜愈薄反應時間愈短,在長期穩定度上以15M/0.8CD-deposited電極的電位漂移量最小,僅為0.33 ± 0.12 mV/hr。藉由整合晶片上的IrOx參考電極與微通道鹽橋的連結,晶片式溶氧與pH電極可同時量測,並無互相cross talk的現象,使量測上呈現良好再現性。在晶片操作模式下,在250秒後得到穩定擴散限制電流,且在低於20 μL/min流速下,不干擾整體量測。此電極晶片化的整合所發展之細胞晶片感測器,未來可用於評估不同藥物對細胞影響的程度,可增加藥物在動物或臨床試驗的速度與減少成本的花費。
Recently, the cell-based biosensors fabricated by microfabrication techniques have attracted a wide interest. The cellular respiration and acidification rate can be estimated by the change in the dissolved oxygen concentration and the pH. In this study, the microfabricated non-Clark type oxygen sensors and the electrodeposited IrOx-pH electrodes were used for the measurement of cellular respiratory and acidification, respectively. The sensitivity and thickness of IrOx electrodes electrodeposited in the range of 0.6~1.0 mA/cm2 current density (CD) doesn’t present significant difference. To Compare the effect of different electrodepositing time of 5 min (5M/0.8CD-), 10 min (10M/0.8CD-) and 15 min (15M/0.8CD-) IrOx electrodes electrodeposited at 0.8 mA/cm2 on the response time, the 90% response time were 2.2 ± 0.9 s, 7.1 ± 0.7 s and 12.8 ± 1.6 s, respectively. The result reveals that the thinner the film is, the shorter the response time becomes. However, in long-term stability test the 15M/0.8CD-depostied IrOx-pH exhibited the smallest potential drifting of 0.33 ± 0.12 mV/hr. With the utilization of on-chip IrOx reference electrode and the salt bridge microchannel, the chip-type oxygen and pH electrodes could measure simultaneously without cross talk and have great reproducibility. The diffusion-limited current could reach stable after applying reducing potential for 250 s. Moreover, the O2 and pH measurement was not affected by the convection when the flow rate was less than 20 μL/min. The chip-type cell-based biosensor can be used to estimate the effect of drugs on the cellular physiological behavior in the future, resulting in promoting the progress of drug tests on the experiments of animal and clinic diagnosis and reducing the cost.
其他識別: U0005-1608201214370400
Appears in Collections:生物產業機電工程學系



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