Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3580
標題: 多層璧奈米碳管/聚乙烯醇複合材料薄膜做為生物感測器之探討
Biosensors Based on Multiwalled Carbon Nanotubes/Poly(vinyl alcohol) Composite Film
作者: 黃俊德
Huang, Jing-Dae
關鍵字: multiwalled carbon nanotube
多層壁奈米碳管
Polyvinyl alcohol
聚乙烯醇
出版社: 化學工程學系所
引用: [1] S. Iijima, Nature, 354, 56, 1991. [2] P.J. Britto, K.S.V. Santhanam, P.J. Ajayan, Bioelectrochem. Bioenerg., 41, 121, 1996. [3] S.G. Wang, Q. Zhang, R. Wang, S.F. Yoon, Biochem. Biophys. Res. Commun., 311, 572, 2003. [4] V. Christophe, F. Slivia, T.M. Canh, Talanta, 59, 535, 2003. [5] Q. Zaho, L. Guan, Z. Gu, Q. Zhuang, Electroanalysis, 17, 85, 2004. [6] M. Musameh, J. Wang, A. Merkoci, Y. Lin, Electrochem. Commun., 4, 743, 2002. [7] M. Teresa, O. Gerard, D.M. Brian, O. Richard, Sensor and Actuators B, 41, 89, 1997. [8] S. Kelly, D. Compagnone, G. Guilbault, Biosens. Bioelectron., 13, 173, 1998. [9] S.B. Sobolov, M.D. Leonida, K.I. Voivodov, J. Org. Chem., 61, 2125, 1996. [10] C.C. Pang, Min.H. Chen, T.Y. Lin, T.C. Chou, Sensor and Actuators B, 73, 221, 2001. [11] P. C. Pandey, S. Upadhyay, Ida Tiwari, V. S. Tripathi, Anal. Biochem., 288, 39, 2001. [12] X. Yu, D. Chattopadhyay, I. Galeska, F. Papadimitrakopoulos, J.F. Rusling, Electrochem. Commun., 5, 408, 2003. [13] M. Umana, J. Waller, Anal. Chem., 58, 2979, 1986. [14] M.C. Shin, H.S. Kim, Biosens. Bioelectron., 11, 171, 1996. [15] K. Yamamoto, G.Y. Shi, T.S. Zhou, F. Xu, J.M. Xu, T. Kato, J.Y. Jin, L.T. Jin, Analyst, 128, 249, 2003. [16] X. Yu, G. A. Sotzing, F. Papadimitrakopoulos, J. F. Rusling, Anal. Chem., 75, 4565, 2003. [17] K. Wu, J. Fei, S. Hu, Anal. Biochem., 318, 100, 2003. [18] T. N. Rao, I. Yagi, T. Miwa, D.A. Tryk, A. Fujishima, Anal. Chem., 71, 2506, 1999. [19] K.P. Troyer, R.M. Wightman, Anal. Chem., 74, 5370, 2002. [20] J. Wang, M. Li, Z. Shi, N. Li, Z. Gu, Microchem. J., 73, 325, 2002. [21] T. Nakaminami, S. Kuwabata, H. Yoneyama, Anal. Chem., 71, 1928, 1999. [22] J. Wang, M.P. Chatrathi, B. Tian, R. Polsky, Anal.Chem., 72, 2514, 2000. [23] P.B. Wimer, K. Pang, G.M. Rose, G.A. Gerhardt, Brain res., 558, 305, 1991. [24] Y.D. Zhao, W.D. Zhang, H. Chen, Q.M. Luo, Sens. Actuators B, 92, 279, 2003. [25] M.F.S. Teixeira, E.R. Dockal, É.T.G. Cavalheiro, Sens. Actuators B, 106, 619, 2005. [26] H. Cai, X.N. Cao, Y. Jiang, P.G. He, Anal. Bioanal. Chem., 375, 287, 2003. [27] X. Mao, L. Yang, X.L. Su, Y. Li, Biosens. Bioelectron., 21, 1178, 2006. [28] J. Wang, M. Li, Z. Shi, N. Li, Z. Gu, Anal. Chem., 74, 1993, 2002. [29] T. Ishihara, T. Arakawa, Sens. Actuators B, 91, 262, 2003. [30] B. Ge, F. Lisdat, Anal. Chim. Acta., 454, 53, 2002. [31] K.V. Gobi, F. Mizutani, J. Electroanal. Chem., 484, 172, 2000. [32] M.C. Bank, R.R. Moore, T.J. Davies, R.G. Compton, Chem. Commu., 1805, 2004. [33] R.R. Moore, C.E. Banks, R.G. Compton, Anal. Chem.,76, 2677, 2004. [34] J. Wang, M. Musameh, A. Merkoci, Y. Lin, Electrochem. Commu., 4, 743, 2002. [35] M.F. Islam, E. Rojas, D.M. Bergey, A.T. Johnson, A.G. Yodh, Nano Lett., 3, 269, 2003. [36] M. Zhang, A. Smith, W. Gorski, Anal. Chem., 76, 5045, 2004. [37] 馬心婷,名醫的糖尿病聖經,杏一醫療,2005. [38] M. Alvarez-Icaza, U. Bilitewski, Anal. Chem., 65, 525, 1993. [39] M. Quinto, I. Losito, F. Palmisano, C. G. Zambonin, Anal. Chim. Acta., 420, 9, 2000. [40] A.P. Robert, Med. Instrum., 51. [41] Michael, J. O’Brien II, S.R.J. Brueck, V.H. Perez-Luna, L.M. Tender, G.P. Lopez, Biosens. Bioelectron., 14, 145, 1999. [42] 蔡娟君,國人檳榔、菸、酒使用與氣喘、心血管疾病消化性潰瘍之相關性探討,高雄醫學大學口腔醫學院,2004。 [43] 傅幸梅,酒精對駕駛行為績效影響之研究,國立雲林科技大學工業工程與管理所,2004。 [44] K. TOKO,Biomimetic Seneor Technology,2000. [45] B.R. Eggins, Chemical Sensors and Biosensors, Wiley, New York, 2002. [46] 周昌弘,微機電元件設計與製造教學與研究整合計劃,屏東科技大學,2005。 [47] L.C. Jr Clark, C. Lyons, Ann. N.Y. Acad. Sci., 102, 29, 1962. [48] J. Shah, E. Wilkins, Electroanalysis, 15, 157, 2002. [49] BiochemistryBasic: http://juang.bst.ntu.edu.tw/BCbasics/Enzyme12.htm [50] R. Boyer, Concepts in Biochemistry 2nd, John Wiely & Sons, Inc. [51] F. Scheller, F. Schvbert, Techiques and Instrumentation in Analytical Chemistry, Elserver, 1992. [52] S. Gamati, J.H.T. Luong, A. Mulchandani, Biosens. Bioelectron., 6, 125, 1991. [53] T.P. Jones, M.D. Porter, Anal. Chem., 60, 404, 1988. [54] Z. Zhujun, W.R. Seitz, Anal. Chem., 58, 220, 1986. [55] F.T. Richard, S.S. Jerome, Handbook of Chemical and Biological Sensors, p5. [56] J.C. Vidal, E. Garcia, J.R. Castillo, Sensors and Actuators B, 57,219,1999. [57] U. Mirtha, W. Jess, Anal. Chem., 58,2979,1986. [58] L. Doretti, D. Ferrara, P. Gattolin, S. Lora, Talanta, 44, 859, 1997. [59] G.T. Constantinos, B.F. Ageliki, N.T. Pantelis, Electro. Commu., 7,781,2005. [60] D. Lucio, F. Daniela, G. Paola, L. Silvano, Talanta, 44,859,1997. [61] J. Parellada, A. Narvaez, Biosens. Bioelectron., 12, 267, 1997. [62] J. Li, L.S. Chia, N.K. Goh, S.N. Tan, J. Electroanal. Chem., 460, 234, 1999. [63] J.M.S. Cabral, J.F. Kennedy, R. F. Taylor(New York; Dekker), 73,1991. [64] E. Tamiya, Y. Siura, A. AkiyamA, Ann. NY Acad. Sci, 613,396,1990. [65] Rice University: Rick Smalley’s Group Home Page-Image Gallery. http://smalley.rice.edu/index.cfm [66] C.N.R. Rao, B.C. Satishkumar, A. Govindaraj, M. Nath, Chemphyschem, 2, 78, 2001. [67] D.S. Bethune, C.H. Kiang, M.S. Devries, G. Gorman, R. Savoy, J. Vazquez, Nature, 363, 605, 1993. [68] C.N.R. Rao, B.C. Satishkumar, A. Govindaraj, M. Nash, Chem. Phys. Chem., 2, 78, 2001. [69] M.M.J. Treacy, T.W. Ebbesen, J.M. Gibson, Nature, 381,678,1996. [70] E.W. Wong, P.E. Sheehan, C.M. Lieber, Science, 2 77,1971,1997. [71] M.F. Yu, O. Lourie, M. Dyer, K. Moloni, T. Kelly, Science, 287,637,2000. [72] M. Terrones, W. K. Hsu, H.W. Kroto, D. R. M. Walton, Topics in Current Chemistry, 1991,1,1998. [73] J. Hone, M. Whitney, C. Piskoti, A. Zettl, Phys. Rev. B, 59,2514,1999. [74] M. A. Osman, D. Srivastatva, Nanotechnology, 12,21,2001. [75] N. Hamada, S. Sawada, A. Oshiyama, Phys. Rev. Lett., 68,1579,1992. [76] R.Saito, M. Fujita, G. Dresselhaus, M. S. Dresslhaus, Appl. Rev. Lett., 60,2204,1992. [77] R. Saito, M. Fujita, G. Dresselhaus, M.S. Dresselhaus, Appl. Rev. Lett., 60, 2204, 1992. [78] J.W.G. Wildoer, L.C. Venema, A.G. Rinzler, R.E. Smalley, C. Dekker, Nature, 391, 59, 1998. [79] J. W. G. Wildoer, L. C. Venema, A. G. Rinzler, C. Dekker, Nature, 391,59,1998. [80] T. W. Ebbesen, H. J. Lezec, H. Hiura, J. W. Bennett, H. F. Ghaemi, T. Thio, Nature, 382,54,1996. [81] J. E. Fischer, Acc. Chem. Res., 35,1079,2002. [82] C. Liu, Y.Y. Fan, M. Liu, M.S. Dresselhaus, H.T. Cong, Science, 286, 1127, 1999. [83] R.P. Baldwin, Talanta, 38, 1, 1991. [84] J. Wang, Electroanalysis, 17, 7, 2005. [85] M. Musameh, J. Wang, A. Merkoci, Y. Lin, Electrochem. Commun., 4, 743, 2002. [86] J. Wang, M. Musameh, Y. Lin, J. Am. Chem. Soc., 125, 2408, 2003. [87] J. Wang, M. Musameh, Anal. Chem., 75, 2075, 2003. [88] A. Hirsch, Angew. Chem. Int. Ed., 41, 1853, 2002. [89] J. Liu, A.G. Rinzler, H. Dai, J.H. Hafner, R.K. Bardley, P.J. Boul, A. Lu, T. Iverson, K. Shelimov, C.B. Huffman, F.R. Macias, Y.S. Shon, T.R. Lee, D.T. Colbert, R.E. Smalley, Science, 280, 1253, 1998. [90] A. Kuznetsova, I. Popova, J.T. Yates, M.J. Bronikowski, C.B. Huffman, J. Liu, R.E. Smally, H.H. Hwu, J.G. Chen, J. Am. Chem. Soc., 123, 10699, 2001. [91] E.T. Mickelson, I.W. Chiang, J.L. Zimmerman, P.J. Boul, J. Lozano, J. Liu, R.E. Smally, R.H. Hauge, J.L. Margrave, J. Phys. Chem. B, 103, 4318, 1999. [92] P.J. Boul, J. Liu, E.T. Mickelson, C.B. Huffman, L.M. Ericson, I.W. Chiang, K.A. Smith, D.T. Colbert, R.H. Hauge, J.L. Margrave, R.E. Smally, Chem. Phys. Lett., 310, 367, 1999. [93] M.J. O’Connell, S.M. Bachilo, C.B. Huffman, V.C. Moore, M.S. Strano, E.H. Haroz, K.L. Rialon, P.J. Boul, W.H. Noon, C. Kittrell, J. Ma, R.H. Hauge, R.B. Weisman, R.E. Smalley, Science, 297, 593, 2002. [94] Y.Y. Sun, K.B. Wu, S.S. Hu, Microchim. Acta, 142, 49, 2003. [95] J.H. Rouse, Langmuir, 21, 1055, 2005. [96] M.J. O’Connell, P. Boul, L.M. Ericson, C. Huffman, Y. Wang, E. Haroz, C. Kuper, J. Tour, K.D. Ausman, R.E. Smalley, Chem. Phys. Lett., 342, 265, 2001. [97] J.E. Riggs, Z. Guo, D.L. Carroll, Y.P. Sun, J. Am. Chem. Soc., 122, 5879, 2000. [98] A. Star, J.F. Stoddart, D. Steuerman, M. Diehl, A. Boukai, E.W. Wong, X. Yang, S.W. Chung, H. Choi, J.R. Heath, Angew. Chem. Int. Ed., 40, 1721, 2001. [99] A. Star, D.W. Steuerman, J.R. Heath, J.F. Stoddart, Angew. Chem. Int. Ed., 41, 2508, 2002. [100] Zhang. X, Liu. T, Sreekumar. T. V, Nano. Lett. 3,1285,2003. [101] Doretti. L, Ferrara. D, Gattolin. P, Silvano. L, Talanta, 45,891,1997. [102] 陳進富, Hofffman, 2002&Martens et al., 2000. [103] Lozinsky. V. I, Plieva. F. M, Enzyme. Microb. Technol. 23, 227,1998. [104] 龔伯涵, PVA-CS水膠製備與組織工程應用之評估. [105] Doretti. L, Ferrara. D, Gattolin. P, Silvano. L, Talanta, 1997,44,859. [106] T.H. Young, W.Y. Chuang, N.K. Yao, L.W. Chen, J. Biomed. Mater. Res., 40, 385, 1998. [107] Y. Li, K.G. Neoh, E.T. Kang, Polymer, 45, 8779, 2004. [108] F.M. Veronese, C. Mammucari, F. Schiavon, O. Schiavon, S. Lora, F. Secundo, A. Chilin, A. Guiotto, Il Farmaco, 56, 541, 2001. [109] A.J. Bard, I.R. Faulkner, Electrochemical Methods: Fundaments and Applications, Wily, New York, 2000. [110] D.R. Crow, Principle and Applications of Electrochemistry,高立,1998。 [111] 胡啟章,電化學原理與方法,五南圖書,2002。 [112] G. Binning, C.F. Quate, Ch. Gerber, Phys. Rev. Lett., 56, 930, 1986. [113] L. Doretti, D. Ferrara, P. Gattolin, S. Lora, Talanta, 44, 859, 1997. [114] R.R. Moore, C.E. Banks, R.G. Compton, Anal. Chem., 76, 2677, 2004. [115] V.I. Lozinsky, F.M. Plieva, Enzyme. Microb. Technol. 23, 227, 1998. [116] R. Ricciardi, F. Auriemma, C.D. Rosa, Macromol. Symp., 222, 49, 2005. [117] J. Wang, Analytical Electrochemistry, Wiley-VCH, 2001. [118] J.H.T. Luong, S. Hrapovic, D. Wang, Electroanalysis, 17, 47, 2005. [119] L.C. Clark, C. Lyons, Acad. Sci., 102, 29, 1962. [120] B. Wang, B. Li, Q. Deng, S. Dong, Anal. Chem., 70, 3170, 1998. [121] K.S. Booksh, B.R. Kowalski, Anal. Chem., 782A, 66, 1994. [122] A.J. Cunningham, Introduction to Bioanalytical Sensors, Wiley, New York, 1998. [123] X. Liu, X. Zhang, H. Liu, T. Yu, J. Deng, J. Biotechnol., 46, 131, 1996. [124] S.G. Wang, Q. Zhang, R. Wang, S.F. Yoon, J. Ahn, D.J. Yang, J.Z. Tian, J.Q. Li, Q. Zhou, Electrochem. Commun., 4, 743, 2002. [125] H.J. Hecht, H.M. Kalisz, J. Hendle, R.D. Schmid, D. Schomburg, J. Mol. Biol., 229, 153, 1993. [126] F. Balavoine, P. Schultz, C. Richard, V. Mallouh, T.W. Ebbesen, CMioskowski, Angew.Chem. Int. Ed., 38, 1912, 1999. [127] B.R. Azamian, J.J. Davis, K.S. Coleman, C.B. Bagshaw, M.L.H. Green, J. Am. Chem. Soc., 124, 12664, 2002. [128] Q. Zhao, Z. Gan, Q. Zhuang, Electroanalysis, 14, 1609, 2002. [129] J.K. Park, H.J. Yee, K.S. Lee, W.W. Lee, M.C. Shin, T.H. Kim, S.R. Kim, Anal. Chim. Acta., 390, 83, 1999. [130] P.C. Pandey, S. Upadhyay, B.C. Upadhyay, H.C. Pathak, Anal. Biochem., 260, 195, 1998. [131] J.K. Park, H.J. Yee, K.S. Lee, W.Y. Lee, M.C. Shin, T.H. Kim, S.R. Kim, Anal. Chim. Acta, 390, 83, 1999. [132] C.X. Cai, K.H. Xue, Y.M. Zhou, H. Yang, Talanta, 44, 339, 1997. [133] M. Jacques, P. J. Elving, J. Am. Chem., 102, 6533, 1980. [134] M. Musameh, J. Wang, A. Merkoci, Y. Lin, Electrochem. Commu., 4, 743, 2002.
摘要: 本研究成功的製備出一種新穎多層璧奈米碳管-聚乙烯醇(MWNTs -PVA)複合薄膜,並應用於葡萄糖生物感測器及酒精生物感測器。多層璧奈米碳管經由高分子纏繞的修飾方式,成功的被穩定分散於經由冷凍-解凍程序(frezzing-thawing process)的聚乙烯醇溶液中,將MWNTs-PVA溶液經由簡單的塗佈方式(casting)製備出MWNTs-PVA複合薄膜修飾電極。由原子力顯微鏡(atomic force microscope , AFM)我們可以觀察到不同含量的MWNTs-PVA修飾電極的表面形貌,經由AFM我們可觀察到由電弧放電法合成的MWNTs為筆直的棒狀結構,其直徑約為40-60 nm且每根MWMTs都均勻分佈在PVA薄膜中,提供相當良好的電傳導性。 將MWNTs-PVA複合薄膜修飾在玻璃碳電極(glassy carbon electrode, GCE)上,並浸於含有5 mM Fe(CN)63-/4-氧化還原物的0.1 M KCl溶液系統中,分別以不同的掃描速度(25~200 mV/s)掃描之循環伏安實驗中,我們可以觀察到隨著掃描速度不斷的增加,除了會使得陽極的峰電流及陰極的峰電流增加,而且陽極峰電流及陰極峰電流分別與掃描速率的平方根成正比的線性關係,因此可以判斷出由MWNTs-PVA複合薄膜修飾GCE所進行的電化學反應屬於擴散控制。 在葡萄糖感測試驗中我們首先利用電化學中的安培法對過氧化氫測試,我們發現MWNTs-PVA複合薄膜修飾GCE其靈敏度比GCE增加了115倍,於是我們開始將不同量的葡萄糖氧化酵素加入MWNTs-PVA中所製作成的葡萄糖生物感測器,其對於葡萄糖的偵測信號有相當大的影響。在葡萄糖生物感測器中GOD最適值為10 mg/mL,其靈敏度533 nA/mM、線性範圍達2 mM、偵測極限75 μM及應答時間小於5秒。 在酒精感測試驗中,我們同樣利用電化學中的安培法對NADH測試,發現MWNTs-PVA複合薄膜對於NADH具有很高的電流訊號而且呈線性比例。於是我們將不同量的酒精去氫酶加入MWNTs-PVA中所製作成的酒精生物感測器,其對於酒精的偵測信號有相當大的影響。酒精生物感測器中ADH最適值為5 mg/mL,其靈敏度196 nA/mM、線性範圍達1.5 mM、偵測極限13 μM及應答時間小於8秒。
Multiwalled carbon nanotubes (MWNTs) were directly dispersed into poly(vinyl alcohol) (PVA) aqueous solution without functionalization of their surface. A MWNTs-PVA nanocomposite film is prepared by cast deposition from a homogeneous MWNTs-PVA solution. The MWNTs is miscible within MWNTs-PVA nanocomposite which is characterized by atomic force microscopy (AFM). The MWNTs are cylindrical with a diameter in the range of 40 - 60 nm and provide electrical conductivity. Well-defined voltammetric responses are observed for the Fe(CN)64-/Fe(CN)63- redox system in aqueous 0.1 M KCl at MWNTs-PVA nanocomposite film modified glassy carbon electrode. In contrast to electrode coated only with PVA, both anodic and cathodic peak currents depended linearly on the square root of the scan rate over the range of 25 - 200 mV/s at MWNTs-PVA nanocomposite film modified electrode, which suggests diffusion-control and fast electron conduction within the film. Amperometric transducers fabricated with these materials were characterized electrochemically using amperometry in the presence of hydrogen peroxide and in the presence of glucose. When compared with bare glassy carbon electrode, the sensitivity to hydrogen peroxide is greatly improved by about 115 times at MWNTs-PVA modified electrode. This glucose biosensor sensitivity was strongly influenced by glucose oxidase concentration within the nanobiocomposite film. The optimized glucose biosensor (10 mg/ml GOD) displayed a sensitivity of 533 nA/mM, a linear range up to 2 mM, a detection limit of 75 μM, and a response time of less than 5 s. The measurement of ethanol is based on the signal produced by β-nicotinamide adenine dinucleotide (NADH), the product of the enzymatic reaction. This ethanol biosensor sensitivity was strongly influenced by alcohol dehydrogenase concentration within the nanobiocomposite film. The optimized ethanol biosensor (5 mg/ml ADH) displayed a sensitivity of 196 nA/mM, a linear range up to 1.5 mM, a detection limit of 13 μM, and a response time of less than 8 s.
URI: http://hdl.handle.net/11455/3580
其他識別: U0005-2306200616404000
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2306200616404000
Appears in Collections:化學工程學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.