Please use this identifier to cite or link to this item:
標題: 從竹嵌紋病毒複製蛋白質及其衛星核酸構成之複製蛋白質複合物中尋找宿主因子
To identify host factors involved in the replication complex of Bamboo mosaic virus replicase and its satellite RNA
作者: 林姿伶
Lin, Tzu-Ling
關鍵字: 本氏菸草
Nicotiana benthamiana
replicase complex
Bamboo mosaic virus
satellite RNA
silver stain
出版社: 生物科技學研究所
引用: Annamalai, P., Hsu, Y.H., Liu, Y.P., Tsai, C.H., Lin, N.S., 2003. Structural and mutational analyses of cis-acting sequences in the 5''-untranslated region of satellite RNA of bamboo mosaic potexvirus. Virology 311, 229-239. Bartels, S., Anderson, J.C., Gonzalez Besteiro, M.A., Carreri, A., Hirt, H., Buchala, A., Metraux, J.P., Peck, S.C., Ulm, R., 2009. MAP kinase phosphatase1 and protein tyrosine phosphatase1 are repressors of salicylic acid synthesis and SNC1-mediated responses in Arabidopsis. Plant Cell 21, 2884-2897. Berger, K.L., Cooper, J.D., Heaton, N.S., Yoon, R., Oakland, T.E., Jordan, T.X., Mateu, G., Grakoui, A., Randall, G., 2009. Roles for endocytic trafficking and phosphatidylinositol 4-kinase III alpha in hepatitis C virus replication. Proc Natl Acad Sci USA 106, 7577-7582. Boon, J.A.d., Diaz, A., Ahlquist, P., 2010. Cytoplasmic viral replication complexes. Cell Host and Microbe review 8, 77-85. Chang, B.Y., Lin, N.S., Liou, D.Y., Chen, J.P., Liou, G.G., Hsu, Y.H., 1997. Subcellular localization of the 28 kDa protein of the triple-gene-block of bamboo mosaic potexvirus. J Gen Virol 78, 1175-1179. Chen, H.C., Hsu, Y.H., Lin, N.S., 2007. Downregulation of bamboo mosaic virus replication requires the 5'' apical hairpin stem loop structure and sequence of satellite RNA. Virology 365, 271-284. Cheng, C.P., Tsai, C.H., 1999. Structural and functional analysis of the 3'' untranslated region of bamboo mosaic potexvirus genomic RNA. J Mol Biol 288, 555-565. Cheng, C.W., Hsiao, Y.Y., Wu, H.C., Chuang, C.M., Chen, J.S., Tsai, C.H., Hsu, Y.H., Wu, Y.C., Lee, C.C., Meng, M., 2009. Suppression of bamboo mosaic virus accumulation by a putative methyltransferase in Nicotiana benthamiana. J Virol 83, 5796-5805. Cheng, S.F., Huang, Y.P., Wu, Z.R., Hu, C.C., Hsu, Y.H., Tsai, C.H., 2010. Identification of differentially expressed genes induced by bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism. BMC Plant Biol 10, 1-12. Cheval, C., Aldon, D., Galaud, J.P., Ranty, B., 2013. Calcium/calmodulin-mediated regulation of plant immunity. Biochim Biophys Acta 1833, 1766-1771. Chou, Y.L., Hung, Y.J., Tseng, Y.H., Hsu, H.T., Yang, J.Y., Wung, C.H., Lin, N.S., Meng, M., Hsu, Y.H., Chang, B.Y., 2013. The stable association of virion with the triple-gene-block protein 3-based complex of bamboo mosaic virus. PLoS Pathog 9, e1003405. Christine Kammel, Maren Thomaier, Brian B. Sorensen, Thomas Schubert, Gernot Langst, Marion Grasser, Grasser, K.D., 2013. Arabidopsis DEAD-Box RNA helicase UAP56 interacts with both RNA and DNA as well as with mRNA export factors. PLoS One 8, e60644. Goh, P.Y., Tan, Y.J., Lim, S.P., Tan, Y.H., Lim, S.G., Fuller-Pace, F., Hong, W., 2004. Cellular RNA helicase p68 relocalization and interaction with the hepatitis C virus (HCV) NS5B protein and the potential role of p68 in HCV RNA replication. J Virol 78, 5288-5298. Harris, K.S., Xiang, W., Alexander, L., Lane, W.S., Paul, A.V., Wimmer, E., 1994. Interaction of poliovirus polypeptide 3CDpro with the 5'' and 3'' termini of the poliovirus genome identification of viral and cellular cofactors needed for efficient binding. J Biol Chem 269, 27004-27014. Heo, J.O., Chang, K.S., Kim, I.A., Lee, M.H., Lee, S.A., Song, S.K., Lee, M.M., Lim, J., 2011. Funneling of gibberellin signaling by the GRAS transcription regulator scarecrow-like 3 in the Arabidopsis root. Proc Natl Acad Sci USA 108, 2166-2171. Hsu, Y. H., Lee, Y. S., Liu, J. S., Lin, N. S., 1998. Differential interactions of bamboo mosaic potexvirus satellite RNAs, helper virus, and host plants. Mol Plant Microbe Interact 11, 1207-1213. Hsu, Y.H., Chen, H.C., Cheng, J., Annamalai, P., Lin, B.Y., Wu, C.T., Yeh, W.B., Lin, N.S., 2006. Crucial role of the 5'' conserved structure of bamboo mosaic virus satellite RNA in downregulation of helper viral RNA replication. J Virol 80, 2566-2574. Huang, Y.L., Han, Y.T., Chang, Y.T., Hsu, Y.H., Meng, M., 2004. Critical residues for GTP methylation and formation of the covalent m7GMP-enzyme intermediate in the capping enzyme domain of bamboo mosaic virus. J Virol 78, 1271-1280. Huang, Y.W., Hu, C.C., Liou, M.R., Chang, B.Y., Tsai, C.H., Meng, M., Lin, N.S., Hsu, Y.H., 2012. Hsp90 interacts specifically with viral RNA and differentially regulates replication initiation of bamboo mosaic virus and associated satellite RNA. PLoS Pathog 8, e1002726. Kopek, B.G., Perkins, G., Miller, D.J., Ellisman, M.H., Ahlquist, P., 2007. Three-dimensional analysis of a viral RNA replication complex reveals a virus-induced mini-organelle. PLoS Biol 5, e220. Kovalev, N., Pogany, J., Nagy, P.D., 2012. A Co-Opted DEAD-Box RNA helicase enhances tombusvirus plus-strand synthesis. PLoS Pathog 8, e1002537. Lancien, M., Gadal, P., Hodges, M., 1998. Molecular characterization of higher plant NAD-dependent isocitrate dehydrogenase: evidence for a heteromeric structure by the complementation of yeast mutants. Plant J 16, 325-333. Li, Q., Brass, A.L., Ng, A., Hu, Z., Xavier, R.J., Liang, T.J., Elledge, S.J., 2009. A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Proc Natl Acad Sci USA 106, 16410-16415. Li, Y.I., Chen, Y.J., Hsu, Y.H., Meng, M., 2001a. Characterization of the AdoMet-dependent guanylyltransferase activity that is associated with the N terminus of bamboo mosaic virus replicase. J Virol 75, 782-788. Li, Y.I., Shih, T.W., Hsu, Y.H., Han, Y.T., Huang, Y.L., Meng, M., 2001b. The helicase-like domain of plant potexvirus replicase participates in formation of RNA 5'' cap structure by exhibiting RNA 5''-triphosphatase activity. J Virol 75, 12114-12120. Li, Y.J., Cheng, Y.M., Huang, Y.L., Tsai, C.H., Hsu, Y.H., Meng, M., 1998. Identification and characterization of the Escherichia coli-expressed RNA-dependent RNA polymerase of bamboo mosaic virus. J Virol 72, 10093-10099. Lin, J.W., Ding, M.P., Hsu, Y.H., Tsai, C.H., 2007. Chloroplast phosphoglycerate kinase, a gluconeogenetic enzyme, is required for efficient accumulation of bamboo mosaic virus. Nucleic Acids Res 35, 424-432. Lin, L., Li, Y., Pyo, H.M., Lu, X., Raman, S.N., Liu, Q., Brown, E.G., Zhou, Y., 2012. Identification of RNA helicase A as a cellular factor that interacts with influenza A virus NS1 protein and its role in the virus life cycle. J Virol 86, 1942-1954. Lin, M.K., Chang, B.Y., Liao, J.T., Lin, N.S., Hsu, Y.H., 2004. Arg-16 and Arg-21 in the N-terminal region of the triple-gene-block protein 1 of bamboo mosaic virus are essential for virus movement. J Gen Virol 85, 251-259. Lin, M.T., Kitajima, E.W., Cupertino, F.P., Costa, C.L., 1977. Partial purification and some properties of bamboo mosaic virus. Annu Rev of Phytopathol 67, 1439-1443. Lin, N.S., Hsu, Y.H., 1994. A satellite RNA associated with bamboo mosaic potexvirus. Virology 202, 707-714. Lin, N.S., Lee, Y.S., Lin, B.Y., Lee, C.W., Hsu, Y.H., 1996. The open reading frame of bamboo mosaic potexvirus satellite RNA is not essential for its replication and can be replaced with a bacterial gene. Proc Natl Acad Sci USA 93, 3138-3142. Lin, N.S., Lin, B.Y., Lo, N.W., Hu, C.C., Chow, T.Y., Hsu, Y.H., 1994. Nucleotide sequence of the genomic RNA of bamboo mosaic potexvirus. J Gen Virol 75, 2513-2518. Liu, J.S., Hsu, Y.H., Huang, T.Y., Lin, N.S., 1997. Molecular evolution and phylogeny of satellite RNA associated with bamboo mosaic potexvirus. J Mol Evol 44, 207-213. Liu, J.S., Lin, N.S., 1995. Satellite RNA associated with bamboo mosaic potexvirus shares similarity with satellites associated with sobemoviruses. Arch Virology 140, 1511-1514. Ma, B., Qian, D., Nan, Q., Tan, C., An, L., Xiang, Y., 2012. Arabidopsis vacuolar H+-ATPase (V-ATPase) B subunits are involved in actin cytoskeleton remodeling via binding to, bundling, and stabilizing F-actin. J Biol Chem 287, 19008-19017. Miller, D.J., Schwartz, M.D., Ahlquist, P., 2001. Flock house virus RNA replicates on outer mitochondrial membranes in Drosophila cells. J Virol 75, 11664-11676. Nagy, P.D., Pogany, J., 2012. The dependence of viral RNA replication on co-opted host factors. Nat Rev Microbiol 10, 137-149. Nishikiori, M., Mori, M., Dohi, K., Okamura, H., Katoh, E., Naito, S., Meshi, T., Ishikawa, M., 2011. A host small GTP-binding protein ARL8 plays crucial roles in tobamovirus RNA replication. PLoS Pathog 7, e1002409. Noueiry, A.O., Ahlquist, P., 2003. Brome mosaic virus RNA replication: revealing the role of the host in RNA virus replication. Annu Rev of Phytopathol 41, 77-98. Olinares, P.D., Kim, J., van Wijk, K.J., 2011. The Clp protease system; a central component of the chloroplast protease network. Biochimi Biophys Acta 1807, 999-1011. Paquette, A.J., Benfey, P.N., 2005. Maturation of the ground tissue of the root is regulated by gibberellin and SCARECROW and requires short-root. Plant Physiol 138, 636-640. Peleman, J., Boerjan, W., Engler, G., Seurinck, J., Botterman, J., Alliotte, T., Van Montagu, M., Inze, D., 1989. Strong cellular preference in the expression of a housekeeping gene of Arabidopsis thaliana encoding S-adenosylmethionine synthetase. Plant Cell 1, 81-93. Peng, J., Yang, J., Yan, F., Lu, Y., Jiang, S., Lin, L., Zheng, H., Chen, H., Chen, J., 2011. Silencing of NbXrn4 facilitates the systemic infection of tobacco mosaic virus in Nicotiana benthamiana. Virus Res 158, 268-270. Perera, R., Daijogo, S., Walter, B.L., Nguyen, J.H., Semler, B.L., 2007. Cellular protein modification by poliovirus: the two faces of poly(rC)-binding protein. J Virol 81, 8919-8932. Perochon, A., Aldon, D., Galaud, J.P., Ranty, B., 2011. Calmodulin and calmodulin-like proteins in plant calcium signaling. Biochimie 93, 2048-2053. Prasanth, K.R., Huang, Y.W., Liou, M.R., Wang, R.Y., Hu, C.C., Tsai, C.H., Meng, M., Lin, N.S., Hsu, Y.H., 2011. Glyceraldehyde 3-phosphate dehydrogenase negatively regulates the replication of bamboo mosaic virus and its associated satellite RNA. J Virol 85, 8829-8840. Quinkert, D., Bartenschlager, R., Lohmann, V., 2005. Quantitative analysis of the hepatitis C virus replication complex. J Virol 79, 13594-13605. Rymarquis, L.A., Souret, F.F., Green, P.J., 2011. Evidence that XRN4, an Arabidopsis homolog of exoribonuclease XRN1, preferentially impacts transcripts with certain sequences or in particular functional categories. RNA 17, 501-511. Sade, D., Brotman, Y., Eybishtz, A., Cuadros-Inostroza, A., Fernie, A.R., Willmitzer, L., Czosnek, H., 2013. Involvement of the hexose transporter gene LeHT1 and of sugars in resistance of tomato to tomato yellow leaf curl virus. Mol plant. doi:10.1093/mp/sst036 First published online, February 21, 2013 Salonen, A., Ahola, T., Kaariainen, L., 2005. Viral RNA replication in association with cellular membranes. Curr Top Microbiol Immunol 285, 139-173. Silva, P.A., Pereira, C.F., Dalebout, T.J., Spaan, W.J., Bredenbeek, P.J., 2010. An RNA pseudoknot is required for production of yellow fever virus subgenomic RNA by the host nuclease XRN1. J Virol 84, 11395-11406. Soto-Rifo, R., Rubilar, P.S., Ohlmann, T.o., 2013. The DEAD-box helicase DDX3 substitutes for the cap-binding protein eIF4E to promote compartmentalized translation initiation of the HIV-1 genomic RNA. Nucleic Acids Res 41, 1-14. Spuul, P., Balistreri, G., Hellstrom, K., Golubtsov, A.V., Jokitalo, E., Ahola, T., 2011. Assembly of alphavirus replication complexes from RNA and protein components in a novel trans-replication system in mammalian cells. J Virol 85, 4739-4751. Vijaya Palani, P., Kasiviswanathan, V., Chen, J.C., Chen, W., Hsu, Y.H., Lin, N.S., 2006. The arginine-rich motif of bamboo mosaic virus satellite RNA-encoded P20 mediates self-interaction, intracellular targeting, and cell-to-cell movement. Mol Plant Microbe Interact 19, 758-767. Walter, B.L., Parsley, T.B., Ehrenfeld, E., Semler, B.L., 2002. Distinct poly(rC) binding protein KH domain determinants for poliovirus translation initiation and viral RNA replication. J Virol 76, 12008-12022. Wang, R.Y., Stork, J., Pogany, J., Nagy, P.D., 2009. A temperature sensitive mutant of heat shock protein 70 reveals an essential role during the early steps of tombusvirus replication. Virology 394, 28-38. Wung, C.-H., Hsu, Y.-H., Liou, D.-Y., Huang, W.-C., Lin, N.-S., Chang, B.-Y., 1999. Identification of the RNA-binding sites of the triple gene block proein 1 of bamboo mosaic potexvirus. J Gen Virol 80, 1119-1126. Yamanaka, T., Imai, T., Satoh, R., Kawashima, A., Takahashi, M., Tomita, K., Kubota, K., Meshi, T., Naito, S., Ishikawa, M., 2002. Complete inhibition of tobamovirus multiplication by simultaneous mutations in two homologous host genes. J Virol 76, 2491-2497. Yamanaka, T., Ohta, T., Takahashi, M., Meshi, T., Schmidt, R., Dean, C., Naito, S., Ishikawa, M., 2000. TOM1, an Arabidopsis gene required for efficient multiplication of a tobamovirus, encodes a putative transmembrane protein. Proc Natl Acad Sci USA 97, 10107-10112. Yang, C. C., Liu, J. S., Lin, C. P., Lin, N. S., 1997. Nucleotide sequence and phylogenetic analysis of a bamboo mosaic potexvirus isolate from common bamboo (Bambusa vulgaris McClure). Bot Bull Acad Sin 38, 77-84.
摘要: 竹嵌紋病毒 (Bamboo mosaic virus, BaMV) 為一正股 RNA 病毒,基因體全長約 6.4-kb,包含五個轉譯架構 (open reading frames, ORFs)。ORF1 可轉譯出病毒核酸複製酵素,從 N 端到 C 端分別為戴帽酵素活性區 (capping enzyme domain)、類解旋酵素活性區 (helicase-like domain) 及核醣核酸聚合酵素活性區 (RNA-dependent RNA polymerase, RdRp)。竹嵌紋病毒衛星核酸 (BaMV satellite RNA, satBaMV) 為 0.83-kb 之正股 RNA,目前已知 satBaMV 的複製需仰賴 BaMV。本實驗研究目的,是欲尋找與病毒複製有關的宿主蛋白,藉此了解病毒的複製機制。研究方法是以農桿菌 (Agrobacterium tumefaciens) 攜帶 satBaMV RNA 及 BaMV ORF1接種於煙草植物葉片,感染後一至五日分別收取葉片。植物萃取液經 30,000 × g 離心取沉澱之膜組織 (P30),以單獨感染 satBaMV RNA 葉片為對照組,結果顯示感染後第二天的複製酵素表現量最多。接著再以含 N-十二烷基肌氨酸鈉緩衝液從 P30 溶出含有複製酵素的蛋白質,藉由 RdRp 上的HA-tag 作為抗原進行免疫沉澱法 (immuno-precipitation)。經過梯度電泳 (Tricine SDS-4~13% PAGE) 與銀染呈色後,由液態層析串聯質譜儀 (LC/MS/MS) 分析,得到多個可能與 BaMV 相關的宿主因子,包含 DEAD-box ATP dependent RNA helicase 7-like, ATP-dependent DNA helicase Q-like 2-like, RNA helicase, exoribonuclease, vacuolar H+-ATPase B subunit, ripening-related protein, pleiotropic drug resistance like protein, putative S-adenosylmethionine synthetase, scarecrow-like protein 5, ClpA regulatory subunit of Clp protease complex, sucrose transporter, isocitrate dehydrogenase-like protein, MAP kinase phosphatase-like protein, disease resistance protein RGA2, calmodulin-binding protein-like protein。目前正以病毒靜默系統 (virus induce gene silence, VIGS) 的實驗觀察這些宿主因子對病毒與衛星核酸複製的影響。
Bamboo mosaic virus (BaMV) is a 6.4-kb positive-sense RNA virus, containing five open reading frames (ORFs). The ORF1 encodes a 155 kDa viral replicase, comprising a capping enzyme, a RNA 5’-triphosphatase and a RNA-dependent RNA polymerase (RdRp) from N- to C- terminus. BaMV satellite RNA (satBaMV) is dependent on BaMV for replication. The purpose of this study is to identify host factors involved in the replication of BaMV. Agrobacterium tumefaciens carrying BaMV replicase and BaMV-Satellite RNA-F4 expression plasmid was used for Nicotiana benthamiana plants infiltration. The leaves harvested at the different time points after infiltration were homogenized in extraction buffer, followed by centrifugation at 30,000 ×g to obtain the membrane fraction (P30). Western blot analysis of P30 showed that the virus replicase accumulated mostly at the second day. P30 was solubilized with sarkosyl. The solubilized proteins were collected by immune-precipitation using a HA-tag antibody and analyzed by SDS-PAGE. Compared to the control, the gel region having differential protein bands were excised and subjected to liquid chromatography-tandem mass spectrometry (LC/MS/MS). The results suggested several hypothetial host factors, including DEAD-box ATP dependent RNA helicase 7-like, ATP-dependent DNA helicase Q-like 2-like, RNA helicase, exoribonuclease, vacuolar H+-ATPase B subunit, ripening-related protein, pleiotropic drug resistance like protein, putative S-adenosylmethionine synthetase, scarecrow-like protein 5, AtClpC, sucrose transporter, isocitrate dehydrogenase-like protein, disease resistance protein RGA2,calmodulin-binding protein-like protein.The effects of these candidates on BaMV replication are being investigated by virus-induce gene silencing (VIGS).
其他識別: U0005-1208201316114300
Appears in Collections:生物科技學研究所



Show full item record
TAIR Related Article

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.