Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3587
標題: 應用液態培養Streptomyces padanus PMS-702生產Fungichromin-油脂及界面活性劑添加效應之探討
Production of fungichromin by Streptomyces padanus PMS-702 in Submerged-Study on fatty acids and surfactants addition effects.
作者: 吳信諺
Wu, Hsin-Yen
關鍵字: Streptomyces padanus
鏈黴菌
polyene macrolide
fungichromin
fed-batch fermentation
lipids
多烯類大環內酯
治黴色基素
饋料醱酵
油脂
界面活性劑
出版社: 化學工程學系所
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摘要: 本研究使用具有抗真菌活性的鏈黴菌Streptomyces padanus PMS-702來進行多烯類大環內酯抗生素fungichromin(治黴色基素)之醱酵生產研究。此抗生素不但存在生物防治潛力(例:對立枯絲核菌及番茄晚疫病有良好抑制效果),還可應用於如腫瘤之臨床醫療上。 本研究主要是於液態培養中,藉由不同油脂及界面劑的添加策略,探討對Streptomyces padanus PMS-702生產fungichromin的影響。於搖瓶實驗結果發現,添加油脂皆可提升fungichromin產量,添加界面活性劑方面,只有主成份以油酸為主的Tween 80及Span 80,有良好提升效果。二階段加料方面,於100 mL之醱酵液中,第一天饋入1 mL之Span80,第四天再饋入10 mL, 5% glucose,至第六天fungichromin產量可達1470mg/L,約為未添加時(fungichromin產量127mg/L)的11.6倍。以相同的醱酵策略於5L醱酵槽中進行培養,於醱酵液3L中,第二天饋入30mL之Span80,第三天饋入150 mL, 20% glucose,再於第八天加入30 mL之 Span80,至醱酵第十二天量測fungichromin,其產量可達2542mg/L,約為未添料(fungichromin產量247mg/L)的10.3倍。
Streptomyces padanus PMS-702 not only has the potential for preventing and curing the organism attack (for example, it can control the diseases of Rhizoctonia damping-off of cabbage and tomato late blight), but also can be applied to clinical medical treatment of tumor. This research is focused on fungichromin production from streptomyces padanus PMS-702 in the submerged culture. Effects of adding different lipids and surfactants on fungichromin production were investigated. In the experiments, it showed that some of the lipids could increase the fungichromin production. On the other hand, surfactants such as Tween 80 and Span 80, mainly composed of olein, could increase the fungichromin production. In the flask test, 1mL of Span80 was added to 100 mL of broth on the first day and 10 mL, 5% of glucose on the fourth day, the fungichromin concentration could reach 1470 mg/L on the sixth day, which is 11.6 times compared to that without any addition (127 mg/L). On the other hand, in 5L fermentor with working volume of 3L, 30mL of Span 80 was added on the second day, 150 mL, 20% of glucose on the third day, and 30 mL of Span 80 on the eighth day, the fungichromin concentration could reach 2542 mg/L on the twelfth day, which is 10.3 times to that without any addition (247 mg/L).
URI: http://hdl.handle.net/11455/3587
其他識別: U0005-2508200601502800
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2508200601502800
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