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dc.contributor.authorWu, Hsin-Yenen_US
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dc.description.abstract本研究使用具有抗真菌活性的鏈黴菌Streptomyces padanus PMS-702來進行多烯類大環內酯抗生素fungichromin(治黴色基素)之醱酵生產研究。此抗生素不但存在生物防治潛力(例:對立枯絲核菌及番茄晚疫病有良好抑制效果),還可應用於如腫瘤之臨床醫療上。 本研究主要是於液態培養中,藉由不同油脂及界面劑的添加策略,探討對Streptomyces padanus PMS-702生產fungichromin的影響。於搖瓶實驗結果發現,添加油脂皆可提升fungichromin產量,添加界面活性劑方面,只有主成份以油酸為主的Tween 80及Span 80,有良好提升效果。二階段加料方面,於100 mL之醱酵液中,第一天饋入1 mL之Span80,第四天再饋入10 mL, 5% glucose,至第六天fungichromin產量可達1470mg/L,約為未添加時(fungichromin產量127mg/L)的11.6倍。以相同的醱酵策略於5L醱酵槽中進行培養,於醱酵液3L中,第二天饋入30mL之Span80,第三天饋入150 mL, 20% glucose,再於第八天加入30 mL之 Span80,至醱酵第十二天量測fungichromin,其產量可達2542mg/L,約為未添料(fungichromin產量247mg/L)的10.3倍。zh_TW
dc.description.abstractStreptomyces padanus PMS-702 not only has the potential for preventing and curing the organism attack (for example, it can control the diseases of Rhizoctonia damping-off of cabbage and tomato late blight), but also can be applied to clinical medical treatment of tumor. This research is focused on fungichromin production from streptomyces padanus PMS-702 in the submerged culture. Effects of adding different lipids and surfactants on fungichromin production were investigated. In the experiments, it showed that some of the lipids could increase the fungichromin production. On the other hand, surfactants such as Tween 80 and Span 80, mainly composed of olein, could increase the fungichromin production. In the flask test, 1mL of Span80 was added to 100 mL of broth on the first day and 10 mL, 5% of glucose on the fourth day, the fungichromin concentration could reach 1470 mg/L on the sixth day, which is 11.6 times compared to that without any addition (127 mg/L). On the other hand, in 5L fermentor with working volume of 3L, 30mL of Span 80 was added on the second day, 150 mL, 20% of glucose on the third day, and 30 mL of Span 80 on the eighth day, the fungichromin concentration could reach 2542 mg/L on the twelfth day, which is 10.3 times to that without any addition (247 mg/L).en_US
dc.description.tableofcontents中文摘要-------------------------------------------------------------------------------- i Abstract---------------------------------------------------------------------------------- ii 目次-------------------------------------------------------------------------------------- iii 表目次----------------------------------------------------------------------------------- iv 圖目次----------------------------------------------------------------------------------- v 一、 緒論----------------------------------------------------------------------------- 1 (一) 前言-------------------------------------------------------------------- 1 二、 文獻回顧----------------------------------------------------------------------- 3 (一) 鏈黴菌----------------------------------------------------------------- 3 1. 放線菌之簡介-------------------------------------------------------- 3 2. 鏈黴菌之分類及特性----------------------------------------------- 3 3. 鏈黴菌之生物病害防治-------------------------------------------- 4 (二) 抗生素--------------------------------------------------------- ------- 7 1. 抗生素之簡介-------------------------------------------------------- 7 2. 抗生素之分類及應用----------------------------------------------- 7 3. 抗生素之製程------------------------------------------------------- 12 4. 提升抗生素產量及其實用價值方法之研究------------------- 13 (三) Fungichromin-------------------------------------------------------- 14 1. Fungichromin之介紹----------------------------------------------- 14 2. Fungichromin之結構與性質-------------------------------------- 15 3. Fungichromin之相關研究----------------------------------------- 15 三、 材料與方法------------------------------------------------------------------- 21 (一) 實驗設備--------------------------------------------------------------21 1. 實驗儀器---------------------------------------------------------------21 2. 實驗藥品---------------------------------------------------------------21 (二) 菌種-------------------------------------------------------------------- 22 1. 菌種來源-------------------------------------------------------------- 22 2. 菌種保存-------------------------------------------------------------- 22 (三) 培養基組成---------------------------------------------------------- 23 1. 前培養培養基-------------------------------------------------------- 23 2. 主培養培養基-------------------------------------------------------- 23 (四) 於搖瓶中之條件設定實驗-----------------------------------------23 1. 不同體積之醱酵----------------------------------------------------- 23 (五) 搖瓶添加物實驗---------------------------------------------------- 23 1. 添加不同油脂-------------------------------------------------------- 23 2. 添加不同界面活性劑----------------------------------------------- 24 (六) 兩階段添加界面活性劑實驗------------------------------------- 24 1. 第一階段添加之機制----------------------------------------------- 24 2. 第二階段添加之機制----------------------------------------------- 24 (七) 醱酵槽批次醱酵條件---------------------------------------------- 25 1. 擋版之影響----------------------------------------------------------- 25 2. 溶氧之影響----------------------------------------------------------- 25 3. 探討附著於槽壁上菌體之影響----------------------------------- 25 4. 多階段添加料之影響----------------------------------------------- 26 (八) 添加介質對觀察fungichromin吸附之效應探討------------- 26 (九) 溫度對fungichromin穩定性之影響---------------------------- 26 (十) 分析方法-------------------------------------------------------------- 26 1. 菌體乾重測定-------------------------------------------------------- 26 2. 殘糖量分析----------------------------------------------------------- 27 3. Fungichromin定量分析-------------------------------------------- 28 4. 醱酵槽溶氧電極之校正-------------------------------------------- 28 5. 醱酵槽酸鹼電極之校正-------------------------------------------- 28 6. 氧氣質傳係數(kla)之量測方法------------------------------- 29 四、 結果與討論-------------------------------------------------------------------- 34 (一) 搖瓶條件探討-------------------------------------------------------- 34 1. 探討醱酵體積之影響----------------------------------------------- 34 2. 探討油脂之影響----------------------------------------------------- 36 3. 探討界面活性劑之影響--------------------------------------------- 42 4. 探討介質之影響------------------------------------------------------ 47 5. 二段式添加方式之影響-------------------------------------------- 52 (二) 醱酵槽條件探討----------------------------------------------------- 56 1. 擋板之影響------------------------------------------------------------- 56 2. 溶氧值之影響---------------------------------------------------------- 59 3. 菌體附著於槽壁之影響---------------------------------------------- 62 4. 多階段添加之影響---------------------------------------------------- 66 (三) Fungichromin特性之探討---------------------------------------- 68 1. 溫度對Fungichromin之影響------------------------------------- 68 五、 結論與展望------------------------------------------------------------------- 70 1. 結論-------------------------------------------------------------------- 70 2. 未來展望-------------------------------------------------------------- 71 參考文獻-------------------------------------------------------------------------------- 72 附錄-------------------------------------------------------------------------------------- 76zh_TW
dc.subjectStreptomyces padanusen_US
dc.subjectpolyene macrolideen_US
dc.subjectfed-batch fermentationen_US
dc.title應用液態培養Streptomyces padanus PMS-702生產Fungichromin-油脂及界面活性劑添加效應之探討zh_TW
dc.titleProduction of fungichromin by Streptomyces padanus PMS-702 in Submerged-Study on fatty acids and surfactants addition effects.en_US
dc.typeThesis and Dissertationzh_TW
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