Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3587
DC FieldValueLanguage
dc.contributor李訓清zh_TW
dc.contributor熊光濱zh_TW
dc.contributor易逸波zh_TW
dc.contributor石信德zh_TW
dc.contributor.advisor劉永銓zh_TW
dc.contributor.author吳信諺zh_TW
dc.contributor.authorWu, Hsin-Yenen_US
dc.contributor.other中興大學zh_TW
dc.date2007zh_TW
dc.date.accessioned2014-06-06T05:32:13Z-
dc.date.available2014-06-06T05:32:13Z-
dc.identifierU0005-2508200601502800zh_TW
dc.identifier.citation1.Hsin-Der Shih, Y.-C. Liu, Feng- Lin Hsu, Vanisree Mulabagal, Rajasekhar Dodda, and JennuWen Huang "Fungichromin: A Substance from Streptomyces padanus PMS-702 with Inhibitory Effects on Rhizoctonia solani." Journal of Agricultural and Food Chemistry, 2003. 51 : p.95-99. 2.薛春生, 化學工程大辭典-默克索引. 1988: 中央圖書出版社. 3.林偉誠, 應用Streptomyces padanus PMS-702 生產fungichromin最適化條件之探討. 2004: 國立中興大學化學工程學系碩士論文. 4.羅淇文 "應用液態培養Streptomyces padanus PMS-702生產fungichromin醱酵槽培養條件探討." 2005 國立中興大學化學工程學系碩士論文. 5.馬文漪 楊柳燕 主編 環境微生物工程 南京大學出版社 6. Buchanan, R. E. and N. E. Gibbons(eds): Bergey,s Manual of Systematic Bacteriology, Vol. 1, Williams and Wilkins, Baltimore, 1974. 7.黃秀梨主編 微生物學 高等教育出版社 1998 8. Baker, K. F.. "Evolving concepts of biological control of plant pathogens." Annual Review of Phytopathology, 1987. 25: p.67-85. 9. Misato, T., Ko, K., and Yamaguchi, I.. "Use of antibiotics in Agriculture." Advances in applied microbiology., 1977. 21: p.53-88. 10. Rodger, P. B. "Potential of biopesticides in agriculture." Pesticide science 1993. 39: p.117-129. 11. Hwang, B. K., Ahn, S. J. and Moon, S. S., Production, purification, and antifungal activity of the antibiotic nucleoside, tubercidin, production by Streptomyces violaceoniger. Canadian Journal of Botany, 1994. 72:p.480-485 12. Rothrock, C. S., and Gottlieb, D. "Role of antibiosis in antagonism of Streptomyces hygroscopicus var. geldanus to Rhizoctonia solani in soil." Canadian Journal of Microbiology, 1984. 30: p.1440-1447. 13. Yuan, W. M., and Crawford, D. L. "Characterization of Streptomyces lydicus WYEC 108 as a potential biocontrol agent against fungal root and seed rots." Applied and Environmental Microbiology, 1995. 61: p.3119-3128. 14. Lahdenpera, M. L., The control of Fusarium wilt on carnation with a Streptomyces preparation. Acta horticulturae, 1987. 2:p.85-92 15. EI-Abyad, M. S., EI-Sayed, M. A., EI-Shanshoury, A. R., and EI-Sabbagh, S. M. "Towards the biological control of fungal and bacterial diseases of tomato using antagonistic Strepmyces spp." Plant Soil, 1993. 149: p.185-195. 16. Emmert, E. A. B., and Handelsman, J. "Biological control of plant disease : a (Gram-) positive perspective." FEMS Microbiology Letters, 1999. 1: p.1-9. 17. Jones, C. R., and Samac, D. A. "Biological control of fungi causing alfalfa seeding damping-off with a disease-suppressive strain of Streptomyces." Biological Control 7: p.196-204. 18. Xiao, K., Kinkel, L. L., and Samac, D. A. "Biological control of Phytophthora root rots on alfalfa and soybean with Streptomyces." Biological Control, 2002. 23: p.285-295. 19. Lloyd, A. M., Barnason, A. R., Roger, S. G., Byrne, M. C., Fraley, R. T., and Horsch, R. B.. "Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens." Science, 1986. p234. 20. 石信德, 黃振文, 劉永銓 and 徐鳳麟, 植物保護用鏈黴菌Streptomyces padanus PMS-702組成物製劑及其製法. 中華民國專利I 224142號, 2004. 21. Demain, A. L. "Microbial natural products: aline and well in 1998." Nature Biotechnology, 1998. 16: p.3-4. 22. 抗生素工業, 工業技術研究院, 化學工業研究所, 1986.7. 23. Martin, J. F. and Demain, A. L., Microbiological reviews Vol. 1980 44 p.230-251 24.Aharonowitz, Y. (1983) in “Bioactive Microbial Products Ⅱ: Development and Production”. (Nisbet, L.J. and Winstanley, D.J. eds.) p.33 Society General Microbiological Academic Press, New York. 25. Liu, C. M. and Schaffner, C. P., Factors Affecting the Production of Candicidin. Arobl. Lagents and Chemotherapy, 1974. 7(2): p.196-202. 26.K. Vu-Trong, S. B. and P.GRAY, Metabolic regulation in Tylosin-producing Streptomyces fradiae: phosphate control of Tylosin biosynthesis. Antimicrobial Agent and Chemistry, 1981. 19(2): p.209-221. 27. Barabas, G. Y. and Szabo, G., Effect of Penicillin on Streptomycin Production by Streptomyces griseus. Antimicrobial agents and Chemistry, 1977. 11(3): p.392-395. 28. Johnson, T. N. and J., M., Production, purification, and characterization of synnematin. Journal of Biochemistry, 1958. 77: p.112-118. 29.隋婉君 “以玉米穗軸為基質利用Streptomyces rimosus TM-55生產地靈 黴素”1993 國立台灣大學農業化學系博士論文 30. Sosaku, I., Kauya, T., Takashi, K., “Immobilization and Stabilization of Chitosanase by Multipoint Attachment to Agar Gel Support.” Journal of Bioscience and Bioengineering. Vol. 93, No. 2002. 2, p.201-206. 31. Senay, A. C., H. Nursevin. Oztop., ”Immobilization of catalase on chitosan film.” Enzyme and Microbial Technology 26. 2000. p.497-501. 32. Omura, S., and Tanaka H. Production, structure and antifungal activity of polyene macrolides. Macrolide antibiotics: chemistry, biology and practice, New York:1984: p.351-404. 33. Martin, J. F. and McDaniel, L. E., Production of polyene macrolide antibiotics. Advances in applied microbiology, 1977. 21: p. 1-52. 34. Pandey, R. C., Guenther, E. C. and Aszalos, A. A., Physicochemical and biological comparison of polyene macrolide antibiot., fungichromin, lagosin and cogomycin. Journal of antibiotics, 1982. 35: p. 988-996. 35. Robison, R. S., Aszalos, A., Karaemer, N. and Giannini, S. M., Production of fungichromin by Streptoverticillium cinnamomeum subsp. cinnamomeum NRRLB-1285. Journal of antibiotics, 1971. 24: p.273. 36. Harrison, P. H., Noguchi, H. and Vederas, J. C., Biosynthesis of polyene antibiotics: intact incorporation of 13C-labeledoctanoate into fungichromin by Streptomyces cellulosae. Journal of the American Chemical Society, 1986. 108: p. 3833-3834. 37. Keilin, D and Hartree E. F. Journal of biochemistry 1948. 42: p.230. 38. Reit,K. V., Review of Measuring Methods and Results in Nonviscous Gas-Liquid Mass Transfer in Stirred Vessel;Industrial & engineering chemistry process design and development, 1979, 18: p.357-364. 39.Wang, D. I. C., Cooney, C. L., Demain, A. L., Dunnill, P., Humphrey, A. E., Lilly, M. D., Fermentation and Enzyme Technology ; John Wiley & Sons Inc. NY, 1979. 40. 石信德, 鏈黴菌PMS-702防治作物病害的功效與其抑菌主要代謝物治黴色基素之鑑定. 2003, 國立中興大學植物病理學系博士論文. 41. Yang, F. C., Ke, Y. F. and Kuo, S. S., Effect of fatty acids on the mycelial growth and polysaccharide formation by Ganoderma lucidum in shake flask cultures. Enzyme and Microbial Technology, 2000. 27: p.295-301. 42. Choi, D. B., Tamura, S., Park, Y. S., Okabe, M., Seriu, Y. and Takeda, S., Efficient Tylosin Production from Streptomyces fradiae Using Rapeseed Oil. Journal of Fermentation and Bioengineering. Vol. 82, No. 1996. 2, p.183-186. 43.周碧溫, 以饋料批次醱酵對舞菇多醣生產之探討. 2003: 大葉大學食品工程系碩士論文. 44.吳國偉, 以饋料批次醱酵進行蟲草菌(CCRC36412)多醣生產之研究. 2003: 大葉大學食品工程系碩士論文. 45. Bessell, C. J., Fletcher, D. L., Mortimer, A. M., Anslow, W. A., Campbell, A. H. and C, W. H., Production of the antibiotic lagosin. British Patent, 1961: p.84711. 46. Yagi, S., Kitai, S. and Kimura, T., Stimulative effect of elemental Sulfur on Siomycin production by Streptomyces sioyaensis. Applied Microbiology, 1971. 21(2): p.153-156. 47.宮衡, 倫世儀. 溶氧控制和pH控制在賴氨酸分批發酵過程中的應用. 無錫輕工業學院學報. 1994第13卷. 第3期.zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/3587-
dc.description.abstract本研究使用具有抗真菌活性的鏈黴菌Streptomyces padanus PMS-702來進行多烯類大環內酯抗生素fungichromin(治黴色基素)之醱酵生產研究。此抗生素不但存在生物防治潛力(例:對立枯絲核菌及番茄晚疫病有良好抑制效果),還可應用於如腫瘤之臨床醫療上。 本研究主要是於液態培養中,藉由不同油脂及界面劑的添加策略,探討對Streptomyces padanus PMS-702生產fungichromin的影響。於搖瓶實驗結果發現,添加油脂皆可提升fungichromin產量,添加界面活性劑方面,只有主成份以油酸為主的Tween 80及Span 80,有良好提升效果。二階段加料方面,於100 mL之醱酵液中,第一天饋入1 mL之Span80,第四天再饋入10 mL, 5% glucose,至第六天fungichromin產量可達1470mg/L,約為未添加時(fungichromin產量127mg/L)的11.6倍。以相同的醱酵策略於5L醱酵槽中進行培養,於醱酵液3L中,第二天饋入30mL之Span80,第三天饋入150 mL, 20% glucose,再於第八天加入30 mL之 Span80,至醱酵第十二天量測fungichromin,其產量可達2542mg/L,約為未添料(fungichromin產量247mg/L)的10.3倍。zh_TW
dc.description.abstractStreptomyces padanus PMS-702 not only has the potential for preventing and curing the organism attack (for example, it can control the diseases of Rhizoctonia damping-off of cabbage and tomato late blight), but also can be applied to clinical medical treatment of tumor. This research is focused on fungichromin production from streptomyces padanus PMS-702 in the submerged culture. Effects of adding different lipids and surfactants on fungichromin production were investigated. In the experiments, it showed that some of the lipids could increase the fungichromin production. On the other hand, surfactants such as Tween 80 and Span 80, mainly composed of olein, could increase the fungichromin production. In the flask test, 1mL of Span80 was added to 100 mL of broth on the first day and 10 mL, 5% of glucose on the fourth day, the fungichromin concentration could reach 1470 mg/L on the sixth day, which is 11.6 times compared to that without any addition (127 mg/L). On the other hand, in 5L fermentor with working volume of 3L, 30mL of Span 80 was added on the second day, 150 mL, 20% of glucose on the third day, and 30 mL of Span 80 on the eighth day, the fungichromin concentration could reach 2542 mg/L on the twelfth day, which is 10.3 times to that without any addition (247 mg/L).en_US
dc.description.tableofcontents中文摘要-------------------------------------------------------------------------------- i Abstract---------------------------------------------------------------------------------- ii 目次-------------------------------------------------------------------------------------- iii 表目次----------------------------------------------------------------------------------- iv 圖目次----------------------------------------------------------------------------------- v 一、 緒論----------------------------------------------------------------------------- 1 (一) 前言-------------------------------------------------------------------- 1 二、 文獻回顧----------------------------------------------------------------------- 3 (一) 鏈黴菌----------------------------------------------------------------- 3 1. 放線菌之簡介-------------------------------------------------------- 3 2. 鏈黴菌之分類及特性----------------------------------------------- 3 3. 鏈黴菌之生物病害防治-------------------------------------------- 4 (二) 抗生素--------------------------------------------------------- ------- 7 1. 抗生素之簡介-------------------------------------------------------- 7 2. 抗生素之分類及應用----------------------------------------------- 7 3. 抗生素之製程------------------------------------------------------- 12 4. 提升抗生素產量及其實用價值方法之研究------------------- 13 (三) Fungichromin-------------------------------------------------------- 14 1. Fungichromin之介紹----------------------------------------------- 14 2. Fungichromin之結構與性質-------------------------------------- 15 3. Fungichromin之相關研究----------------------------------------- 15 三、 材料與方法------------------------------------------------------------------- 21 (一) 實驗設備--------------------------------------------------------------21 1. 實驗儀器---------------------------------------------------------------21 2. 實驗藥品---------------------------------------------------------------21 (二) 菌種-------------------------------------------------------------------- 22 1. 菌種來源-------------------------------------------------------------- 22 2. 菌種保存-------------------------------------------------------------- 22 (三) 培養基組成---------------------------------------------------------- 23 1. 前培養培養基-------------------------------------------------------- 23 2. 主培養培養基-------------------------------------------------------- 23 (四) 於搖瓶中之條件設定實驗-----------------------------------------23 1. 不同體積之醱酵----------------------------------------------------- 23 (五) 搖瓶添加物實驗---------------------------------------------------- 23 1. 添加不同油脂-------------------------------------------------------- 23 2. 添加不同界面活性劑----------------------------------------------- 24 (六) 兩階段添加界面活性劑實驗------------------------------------- 24 1. 第一階段添加之機制----------------------------------------------- 24 2. 第二階段添加之機制----------------------------------------------- 24 (七) 醱酵槽批次醱酵條件---------------------------------------------- 25 1. 擋版之影響----------------------------------------------------------- 25 2. 溶氧之影響----------------------------------------------------------- 25 3. 探討附著於槽壁上菌體之影響----------------------------------- 25 4. 多階段添加料之影響----------------------------------------------- 26 (八) 添加介質對觀察fungichromin吸附之效應探討------------- 26 (九) 溫度對fungichromin穩定性之影響---------------------------- 26 (十) 分析方法-------------------------------------------------------------- 26 1. 菌體乾重測定-------------------------------------------------------- 26 2. 殘糖量分析----------------------------------------------------------- 27 3. Fungichromin定量分析-------------------------------------------- 28 4. 醱酵槽溶氧電極之校正-------------------------------------------- 28 5. 醱酵槽酸鹼電極之校正-------------------------------------------- 28 6. 氧氣質傳係數(kla)之量測方法------------------------------- 29 四、 結果與討論-------------------------------------------------------------------- 34 (一) 搖瓶條件探討-------------------------------------------------------- 34 1. 探討醱酵體積之影響----------------------------------------------- 34 2. 探討油脂之影響----------------------------------------------------- 36 3. 探討界面活性劑之影響--------------------------------------------- 42 4. 探討介質之影響------------------------------------------------------ 47 5. 二段式添加方式之影響-------------------------------------------- 52 (二) 醱酵槽條件探討----------------------------------------------------- 56 1. 擋板之影響------------------------------------------------------------- 56 2. 溶氧值之影響---------------------------------------------------------- 59 3. 菌體附著於槽壁之影響---------------------------------------------- 62 4. 多階段添加之影響---------------------------------------------------- 66 (三) Fungichromin特性之探討---------------------------------------- 68 1. 溫度對Fungichromin之影響------------------------------------- 68 五、 結論與展望------------------------------------------------------------------- 70 1. 結論-------------------------------------------------------------------- 70 2. 未來展望-------------------------------------------------------------- 71 參考文獻-------------------------------------------------------------------------------- 72 附錄-------------------------------------------------------------------------------------- 76zh_TW
dc.language.isoen_USzh_TW
dc.publisher化學工程學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2508200601502800en_US
dc.subjectStreptomyces padanusen_US
dc.subject鏈黴菌zh_TW
dc.subjectpolyene macrolideen_US
dc.subjectfungichrominen_US
dc.subjectfed-batch fermentationen_US
dc.subjectlipidsen_US
dc.subject多烯類大環內酯zh_TW
dc.subject治黴色基素zh_TW
dc.subject饋料醱酵zh_TW
dc.subject油脂zh_TW
dc.subject界面活性劑zh_TW
dc.title應用液態培養Streptomyces padanus PMS-702生產Fungichromin-油脂及界面活性劑添加效應之探討zh_TW
dc.titleProduction of fungichromin by Streptomyces padanus PMS-702 in Submerged-Study on fatty acids and surfactants addition effects.en_US
dc.typeThesis and Dissertationzh_TW
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