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標題: 阿拉伯芥中調控花序分化基因之特性與功能性分析
Identification and characterization of genes in regulating differentiation of inflorescence from Arabidopsis thaliana
作者: 潘韋成
Pan, Wei-Chen
關鍵字: MYB
apical dominance
出版社: 生物科技學研究所
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摘要: 植物利用轉錄因子來因應環境的變化。MYB family 是阿拉伯芥中最大的一群轉錄因子(transcription factor)。本研究選殖並分析阿拉伯芥中的四個R2R3-type MYB基因,分別是AtMYB-like 1-4 四個基因。這四個基因具有相似的胺基酸序列,特別是在MYB domain 胺基酸序列的相似度最高。這四個基因mRNA表現情形相似,而且整個發育時期都有表現。在開花之後,其mRNA在花、花序莖、葉片及根都有表現。大量表現的AtMYBL1會改變花序的形成,有趣的是35S::AtMYBL2 也改變腋生花序的形成,並且喪失了花序莖的頂芽優勢。35S::AtMYB4也有類似於35S::AtMYBL2的特徵。這些結果指出AtMYBL1 、 AtMYBL2與AtMYB4調節了腋生分生組織的分化和花序的形成。在At1g13930 轉殖反股基因植物實驗中發現一個擁有獨特性狀,且在遺傳分析結果可能是單一T-DNA插入造成之隱性突變株,名為asr突變。這個asr突變株植株矮化,帶有捲曲狀異常花序莖以及發育短而分枝少的根部。經由IPCR鑑定發現這個T-DNA的插入位置是在At1g55265基因啟動子(promoter)及At1g55270基因3’ UTR處。這樣的結果指出At1g55265或是At1g55270這兩個基因可能參與調控花序和根部的發育。進一步分析這兩個基因的表現,發覺At1g55265可能是ASR基因。未來將以互補試驗,期望能夠更了解ASR基因在植物中花序形成與調節根部發育的作用機制。
Transcription factors are important in regulating plant responses to environmental stress. In Arabidopsis the MYB family genes is one of the largest transcription factor gene families. We reported here the cloning and characterization of four R2R3-MYB genes, AtMYB-like 1-4 (AtMYBL1-4), from Arabidopsis. These four genes showed high sequence homology especially in the R2R3 domain. They also showed similar expression pattern and their mRNA was expressed throughout the development. After flowering, their mRNA was detected in flowers, inflorescence shoot, leaves, and root. Ectopic expression of AtMYBL1 in Arabidopsis altered inflorescence shoot formation. Interestingly, 35S::AtMYBL2 and AtMYBL4 also altered the formation of lateral inflorescence and lost apical dominance in transgenic plants. The result indicated that AtMYBL1, AtMYBL2,and AtMYBL4 are likely involved in the regulation of differentiation in shoot apical meristem and formation of inflorescence. A recessive mutation, asr, affecting inflorescence and root development was isolate from 35S::At1g13930 antisense transformant of Arabidopsis. The mutation show dwarf phenotype with winding inflorescence and short root. IPCR approach was applied to isolate the sequence flanking the left border of the T-DNA. The result indicated that the T-DNA was inserted at the promoter of At1g55265 and 3'UTR of At1g55270. The analysis of these two genes indiected that Atig55265 may encode the ASR gene. Further, characterized in this study should lead a deeper understanding of the mechanisms regulating differentiation during various developmental processes in plants.
其他識別: U0005-2108200617265500
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