Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36120
標題: 阿拉伯芥中調控花序分化基因之特性與功能性分析
Identification and characterization of genes in regulating differentiation of inflorescence from Arabidopsis thaliana
作者: 潘韋成
Pan, Wei-Chen
關鍵字: MYB
花序
asr
apical dominance
inflorescence
頂芽優勢
出版社: 生物科技學研究所
引用: 參考文獻 廖珮君(2004)Characterization and functional analysis of genes regulating cell division and differentiation in Arabidopsis.國立中興大學生物科技學研究所碩士論文. Aida, M., Ishida, T. and Tasaka, M. (1999) Shoot apical meristem and cotyledon formation during Arabidopsis embryogenesis: interaction among the CUP-SHAPED COTYLEDON and SHOOT MERISTEMLESS genes. Development, 126, 1563-1570. Bender, J. and Fink, G.R. (1998) A Myb homologue, ATR1, activates tryptophan gene expression in Arabidopsis. Proc Natl Acad Sci USA, 95, 5655-5660. Bin, S., Cristina, G.R. and Patricia, S.S. (2002) The LATERAL ORGAN BOUNDARIES gene defines a novel, plant-specific gene family. Plant Physiol, 129, 747-761. Borevitz, J.O., Xia, Y.J., Blount, J., Dixon, R.A. and Lamb, C. (2000) Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell, 12, 2383-2393. Burns, C.G., Ohi, R., Krainer, A.R. and Gould, K.L. (1999) Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing. Proc Natl Acad Sci USA, 96, 13789-13794. Byrne, M.E., Barley, R., Curtis, M., Arroyo, J.M., Dunham, M., Hudson, A. and Martienssen, R.A. (2000) Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis. Nature, 408, 967-971. Daniel, X., Lacomme, C., Morel, J.B. and Roby, D. (1999) A novel myb oncogene homologue in Arabidopsis thaliana related to hypersensitive cell death. Plant J, 20, 57-66. Dörte, M., Gregor, S. and Klaus, T. (2006) Blind homologous R2R3 Myb genes vontrol the pattern of lateral meristem initiation in Arabidopsis. Plant cell, 18, 586-597. Francesca, Q., John, W., Karel, V.D., Woude, E.S., Nick, D.V., Joseph, M. and Ronald, K. (1999) Molecular analysis of the anthocyanin2 gene of petunia and its role in the evolution of flower color. Plant Cell, 11, 1433-1444. Goff, S.A., Cone, K.C., and Chandler, V.L. (1992) Functional analysis of the transcriptional activator encoded by the maize B-gene: evidence for a direct functional interaction between two classes of regulatory proteins. Genes Dev, 6, 864-875. Grbic, V. and Bleecker, A. (2000) Axillary meristem development in Arabidopsis thaliana. Plant J, 21, 215-223. Greb, T., Clarenz, O., Schäfer, E., Müller, D., Herrero, R., Schmitz, G. and Theres, K. (2003) Molecular analysis of the LATERAL SUPPRESSOR gene in Arabidopsis reveals a conserved control mechanism for axillary meristem formation. Genes Dev, 17, 1175-1187. Gregor, S., Edith, T., Filomena, C., Carola, F., Francesco, C. and Klaus, T. (2002) The tomato Blind gene encodes a MYB transcription factor that controls the formation of lateral meristems. Proc Natl Acad Sci USA, 99, 1064-1069. Hirayama, T. and Shinozaki, K. (1996) A cdc5+ homolog of a higher plant, Arabidopsis thaliana. Proc Natl Acad Sci USA, 93, 13371-13376. Hoeren, F.U., Dolferus, R., Wu, Y., Peacock, W.J. and Dennies, E.S. (1998) Evidence for a role for AtMYB2 in the induction of the Arabidopsis thaliana alcohol dehydrogenase gene (ADH1) by low oxygen. Genetics, 149, 479-490. Höfgen, R. and Willmitzer, L. (1988) Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res, 16, 9877 Ito, M., Araki, S., Matsunaga, S., Itoh, T., Nishihama, R., Machida, Y., Doonan, J.H. and Watanabe, A. (2001) G2/M-phase-specific transcription during the plant cell cycle is mediated by c-Myb-like transcription factors. Plant Cell, 13, 1891-1905. Jin, H. and Martin, C. (1999) Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol, 41, 577-585. Jin, H., Cominelli, E., Bailey, P., Parr, A., Mehrtens, F., Jones, J., Tonelli, C., Weisshaar, B. and Martin, C. (2000) Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis. EMBO J, 19, 6150-6161. Klempnauer, K.H., Gonda, T.J. and Bishop, J.M. (1982) Nucleotide sequence of the retroviral leukemia gene v-Myb and its cellular progenitor c-Myb: the architecture of a transduced oncogene. Cell, 31, 453–463 Kranz, H., Scholz, K. and Weisshaar, B. (2000) c-MYB oncogene-like genes encoding three MYB repeats occur in all major plant lineages. Plant J, 21, 231-235. Kranz, H.D., Denekamp, M., Greco, R., Jin, H., Leyva, A., Meissner, R.C., Petroni, K., Urzainqui, A., Bevan, M. and Martin, C. (1998) Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana. Plant J, 16, 263-276. Lee, M.M. and Schiefelbein, J. (1999) WEREWOLF, a MYB-related protein in Arabidopsis, is a position-dependent regulator of epidermal cell patterning. Cell, 24, 473-483. Lee, M.W., Qi, M. and Yang, Y. (2001) A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death. Mol Plant Microbe Interact, 14, 527-535. Lei, X.H., Shen, X., Xu, X.Q. and Bernstein, H.S. (2000) Human Cdc5, a regulator of mitotic entry, can act as a site-specific DNA binding protein. Jour Cell Sci, 113, 4523-4531. Lipsick, J.S. (1996) One billion years of Myb. Oncogene 13, 223–235. Long, J. and Barton, M.K. (2000) Initiation of axillary and floral meristems in Arabidopsis. Dev Biol, 218, 341-353. Martin, C. and Paz-Ares, J. (1997) MYB transcription factors in plants. Trends Genet, 13, 67-73. Ogata, K., Hojo, H., Aimoto, S., Nakai, T., Nakamura, H., Sarai, A., Ishii, S., and Nishimura, Y. (1992) Solution structure of a DNA-binding unit of Myb: a helix-turn-helix-related motif with conserved tryptophans forming a hydrophobic core. Proc Natl Acad Sci USA, 89, 6428-6432. Oppenheimer, D.G., Herman, P.L., Sivakumaran, S., Esch, J. and Marks, M.D. (1991) A MYB gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules. Cell, 67, 483-493. Otsuga, D., DeGuzman, B., Prigge, M.J., Drews, G.N. and Clark, S.E. (2001) REVOLUTA regulates meristem initiation at lateral positions. Plant J, 25, 223-236. Payne, C.T., Zhang, F. and Lloyd, A.M. (2000) GL3 encodes a bHLH protein that regulates trichome development in Arabidopsis through interaction with GL1 and TTG1. Genetics, 156, 1349-1362. Paz-Ares, J., Wienand, U., Peterson, P.A. and Saedler, H. (1986) Molecular cloning of the c1 locus of Zea mays. a locus regulating the anthocyanin pathway. EMBO J, 5, 829-834. Peng, J., Richards, D.E., Hartley, N.M., Murphy, G.P., Devos, K.M., Flintham, J.E., Beales, J., Fish, L.J., Worland, A.J. and Pelica, F. (1999) ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature, 400, 256-261. Ralf, S., Martin, W. and Bernd, W. (2001) The 2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Bio., 4, 447–456. Riechmann, J.L., Heard, J., Martin, G., Reuber, L., Jiang, C.Z., Keddie, J., Adam, L., Pineda, O., Ratcliffe, O.J. and Samaha, R.R. (2000) Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 290, 2105-2110. Romero, I., Fuertes, A., Benito, M.J., Malpical, J.M., Leyva, A. and Paz-Ares, J. (1998) More than 80 R2R3-MYB regulatory genes in the genome of Arabidopsis thaliana. Plant J, 14, 273-284. Rosinski, J.A. and Atchley, W.R. (1998) Molecular evolution of the Myb family of transcription factors: evidence for polyphyletic origin. J Mol Evol, 46, 74-83. Schmitz, G.,and Theres, K. (2005) Theres: Shoot and inflorescence branching. Curr Opin Plant Bio., 8, 506–511 Schumacher, K., Schmitt, T., Rossberg, M., Schmitz, G. and Theres, K. (1999) The Lateral suppressor gene of tomato encodes a new member of the VHIID protein family. Proc Natl Acad Sci USA, 96, 290-295. Stirnberg, P., van, De, S.K. and Leyser, H.M.O. (2002) MAX1 and MAX2 control shoot lateral branching in Arabidopsis. Development, 129, 1131-1141. Stracke, R., Werber, M. and Weisshaar, B. (2001) The R2R3-Myb gene family in Arabidopsis thaliana. Curr Opin Plant Biol , 4, 447–456 Takada, S., Hibara, K., Ishida, T. and Tasaka, M. (2001) The CUP-SHAPED COTYLEDON1 gene of Arabidopsis regulates shoot apical meristem formation. Development, 128, 1127-1135. Thomas, K., Jessica, A., Thomas, M. and Peter, D. (2006) Arabidopsis REGULATOR OF AXILLARY MERISTEMS1 controls a leaf axil stem cell niche and modulates vegetative development. Plant Cell, 18, 598 - 611. Timmermans, M.C.P., Hudson, A., Becraft, P.W. and Nelson, T. (1999) Rough sheath2: a Myb protein that represses knox homeobox genes in maize lateral organ primordia. Science, 284, 151-153. Tsiantis, M., Schneeberger, R., Golz, J.F., Freeling, M. and Langdale, J.A. (1999) The Maize rough sheath2 gene and leaf development programs in monocot and dicot plants. Science, 284, 154-156. Vroemen, C.W., Mordhorst, A.P., Albrecht, C., Kwaaitaal, M.A.C.J. and Vries, S.C. (2003) The CUP-SHAPED COTYLEDON3 gene is required for boundary and shoot meristem formation in Arabidopsis. Plant Cell, 15, 1563-1577. Waites, R., Selvadurai, H.R.N., Oliver, I.R. and Hudson, A. (1998) The PHANTASTICA gene encodes a MYB transcription factor involved in growth and dorsoventrality of lateral organs in Antirrhinum. Cell, 93, 779-789. Yu, E.Y., Kim, S.E., Kim, J.H., Ko, J.H., Cho, M.H. and Chung, I.K. (2000) Sequence-specific DNA recognition by the Myb-like domain of plant telomeric protein RTBP1. J Biol Chem, 275, 24208-24214. Eva, B., Marta, M., Michael, S., Thomas, T., Daniela, S., Gerd, J., and Jirˇı´, F. (2003) Local efflux-dependent auxin gradients as a common module for plant organ formation. Cell, 115, 591–602. Friml, J., Anne, V., Michael, S., Dolf, W., Heinz, S., Thorsten, H., Remko, O., and Gerd, J. (2003) Efflux-dependent auxin gradients establish the apical-basal axis of Arabidopsis. Nature, 426, 147–153. Giorgio, M. and Ida, R. (2002) Light and shade in the photocontrol of Arabidopsis growth. TRENDS in Plant Sci., 7, 399-404. Jianming, L.i., Kyoung, H.N., Dionne, V., and Joanne, C. (2001) BIN2, a new brassinosteroid-insensitive locus in Arabidopsis.. Plant Physiol, 127, 14-22. Liam, D., Kees, J., Viola, W., Paul, L., Scott, P., Keith, R. and Ben, S. (1993) Cellular organisation of the Arabidopsis thaliana root. Development, 119, 71-84. Medford, J.I., Behringer, F.J., Callos, J.D. and Feldmann, K.A. (1992) Normal and abnormal development in the Arabidopsis vegetative shoot apex. Plant Cell, 4, 631-643. Sabatini, S., Beis, D., Wolkenfelt, H., Murfett, J., Guilfoyle, T., Malamy, J., Benfey, P., Leyser, O., Bechtold, N., Weisbeek, P., and Scheres, B. (1999) An auxin-dependent distal organizer of pattern and polarity in the Arabidopsis root. Cell, 99, 463–472.
摘要: 植物利用轉錄因子來因應環境的變化。MYB family 是阿拉伯芥中最大的一群轉錄因子(transcription factor)。本研究選殖並分析阿拉伯芥中的四個R2R3-type MYB基因,分別是AtMYB-like 1-4 四個基因。這四個基因具有相似的胺基酸序列,特別是在MYB domain 胺基酸序列的相似度最高。這四個基因mRNA表現情形相似,而且整個發育時期都有表現。在開花之後,其mRNA在花、花序莖、葉片及根都有表現。大量表現的AtMYBL1會改變花序的形成,有趣的是35S::AtMYBL2 也改變腋生花序的形成,並且喪失了花序莖的頂芽優勢。35S::AtMYB4也有類似於35S::AtMYBL2的特徵。這些結果指出AtMYBL1 、 AtMYBL2與AtMYB4調節了腋生分生組織的分化和花序的形成。在At1g13930 轉殖反股基因植物實驗中發現一個擁有獨特性狀,且在遺傳分析結果可能是單一T-DNA插入造成之隱性突變株,名為asr突變。這個asr突變株植株矮化,帶有捲曲狀異常花序莖以及發育短而分枝少的根部。經由IPCR鑑定發現這個T-DNA的插入位置是在At1g55265基因啟動子(promoter)及At1g55270基因3’ UTR處。這樣的結果指出At1g55265或是At1g55270這兩個基因可能參與調控花序和根部的發育。進一步分析這兩個基因的表現,發覺At1g55265可能是ASR基因。未來將以互補試驗,期望能夠更了解ASR基因在植物中花序形成與調節根部發育的作用機制。
Transcription factors are important in regulating plant responses to environmental stress. In Arabidopsis the MYB family genes is one of the largest transcription factor gene families. We reported here the cloning and characterization of four R2R3-MYB genes, AtMYB-like 1-4 (AtMYBL1-4), from Arabidopsis. These four genes showed high sequence homology especially in the R2R3 domain. They also showed similar expression pattern and their mRNA was expressed throughout the development. After flowering, their mRNA was detected in flowers, inflorescence shoot, leaves, and root. Ectopic expression of AtMYBL1 in Arabidopsis altered inflorescence shoot formation. Interestingly, 35S::AtMYBL2 and AtMYBL4 also altered the formation of lateral inflorescence and lost apical dominance in transgenic plants. The result indicated that AtMYBL1, AtMYBL2,and AtMYBL4 are likely involved in the regulation of differentiation in shoot apical meristem and formation of inflorescence. A recessive mutation, asr, affecting inflorescence and root development was isolate from 35S::At1g13930 antisense transformant of Arabidopsis. The mutation show dwarf phenotype with winding inflorescence and short root. IPCR approach was applied to isolate the sequence flanking the left border of the T-DNA. The result indicated that the T-DNA was inserted at the promoter of At1g55265 and 3'UTR of At1g55270. The analysis of these two genes indiected that Atig55265 may encode the ASR gene. Further, characterized in this study should lead a deeper understanding of the mechanisms regulating differentiation during various developmental processes in plants.
URI: http://hdl.handle.net/11455/36120
其他識別: U0005-2108200617265500
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2108200617265500
Appears in Collections:生物科技學研究所

文件中的檔案:

取得全文請前往華藝線上圖書館

Show full item record
 
TAIR Related Article
 
Citations:


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.