請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/3614
標題: 應用液態培養Ganoderma lucidum生產Polysaccharide-油脂及界面活性劑添加效應之探討
Production of Polysaccharide by Ganoderma lucidum in Submerged-Study on fatty acids and surfactants addition effects.
作者: 洪嘉男
Hung, Chia-Nan
關鍵字: Ganoderma lucidum
submerged culture
出版社: 化學工程學系所
引用: 參考文獻 1. 水野卓、川合正, 菇類的化學、生化學. 1997: 國立編譯館. 2. Mizuno, T., Bioactive biomolecules of mushroom:Food fruction and medicinal effect of mushroom fungi. Food Reviews International, 1995. 11(1): p. 7-21. 3. Bartnicki-Garcia, S., Cell wall chemistry, morphogenesis, and taxonomy of fungi. Annual Review of Microbiology 1968. 22: p. 87-108. 4. Cheung, P.C.K., Dietary fiber content and composition of some cultivated edible mushroom fruiting bodies and mycelia. Journal of Agriculture and Food Chemistry, 1996. 44: p. 468-471. 5. Mizuno, T., Development of an antiumor biological response modifier from Phelliuns Teng(Aphyllophoromycetideae). Journal of Medical Mushroom, 2000. 2(1): p. 21-25. 6. Kosuna, K., polysaccharide useful as biological response modulators and their preparation with Basidiomycetes. European Patent Ep, 1996: p. 733-740. 7. Alexotolus. C. J and Mims, C. W., Introductory mycology. 1979, New York: John Wiley and 121 Sons, Inc. 8. 許瑞祥, 靈芝概論. 1993, 台中,台灣: 萬年出版社. 9. 陸光樑、林忠平、林志彬, 靈芝的科學應用. 1992, 台北: 度假出版社. 10. 王伯徹, 食品工業. 藥用真菌系列報導(一)靈芝. 22(1). 1990: 財團法人食品工業發展研究所.p. 23-30. 11. 王培銘, 食品工業. 食藥用菇液態培養製程之開發. 34(5). 2002: 財團法人食品工業發展研究所. p. 31-35. 12. Edelstein, L., A model for pellet size distributions in submerged mycelial cultures. Journal of Theoretical Biology 1983. 105: p. 427-452. 13. Blanch, H.W. and Bhavaraju, S.M., A model for pellet breakup in fungal fermentations. Journal of Fermentation and Bioengineering, 1976. 54: p. 466-468. 14. Metz, B. and Kossen, N.W.F., The growth of molds in the form of pellets:Literature review. Biotechnology and Bioengineering 1977. 19: p. 781-799. 15. 黃雪芳;劉柯俊;管育慧;董光世;蘇慶華;董大成, 口服靈芝菌絲培養液之抗癌人工轉移作用. 中華癌醫會誌, 1989. 5(1): p. 10-15. 16. 林俊清, 生藥的解說- 靈芝的介紹. 藥學介紹, 1990. 6(3): p. 104-111. 17. Kojima, T., Tabata, K., Itoh, W. and Yanaki, T. , Molecular weight dependence of the antitumor activity of schizophyllan. Agricultural and biological chemistry, 1986. 50: p. 231-232. 18. Mizuno, T., Saito, H., Nishitoba, T., and Kawagishi, H., Antitumor-active snbstance from mushroom. Food Reviews International.1995: 11. p. 23-61. 19. Chihara, G., Hamuro, J., Maeda, Y.Y., Arai, Y., and Fukuoka, F., Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan, from Lentinus edodes (Berk.)SING. Cancer Research, 1970. 30: p. 2776-2781. 20. Tabata, K., Itoh, W., Kojima, T., Kawabate, S., and Misaki, K., Ultrasonic degradation of schizophyllan, an antitumor polysaccharide produced by Schizophyllum commune FRIES. Carbohydrate Research, 1981. 89: p. 121-135. 21. Ng, T., A review of research on the protein-bound polysaccharide (Polysaccharopeptide, PSP) from the mushroom Coriolus versicolor (Basidiomycetes: Polyporaceae). General Pharmacology, 1998. 30: p. 1-4. 22. Lei, L.S. and Lin, Z.B., Effect of Ganoderma polysaccharideson T cell subpopulstions and production of interleukin2 in mixed lymphocyte response. Acta Pharmaceutica Sinica, 1992. 27: p. 331-335. 23. Lei, L.S. and Lin, Z.B., Effect of Ganoderma polysaccharides on the activity of DNA polymerase α in spleen cells stimulated by alloantigens in mice in vitro. Beijing Medical University 1991. 23 p. 329-333. 24. Lieu, C.W., Lee, S.S., and Wang, S.Y., The effect Ganoderma lucidum on induction of differentiation in leukemin U937 cells. Anticancer Research, 1992. 12: p. 1211-1216. 25. Matsuzaki, K., Sato, T., Enomoto, K., Yamamoto, I., Oshima, R., Hatanaka, K., Uryu, T., Kaku, H., Sone, Y., and Misaki, A., Synthesis of water-soluble, branched of polysaccharides having Dmannopyranose, D-arabinofuranose, or oligo-D-arabinofuranose side-chains and their antitumor activity. Carbohydrate Research, 1986. 157: p. 171-183. 26. Sasaki, T., Takasuka, N., Chihara, G., and Maeda, Y., Antitumor activity of degradaded products of lentinan: its correlation with molecular weight. Gann, 1976. 67: p. 191-195. 27. Kishida, E., Sone, Y., and Misaki, A., Effects of branch distribution and chemical modifications of antitumor (1→3)-β-D-glucans. Carbohydrate Polymers 1992. 17: p. 89-95. 28. Bohn, J.A. and Bemiller, J.N., (1→3)-β-D-glucans biological response modifiers: a review of structure-functional activity relationships. Carbohydrate Polymers 1995. 28: p. 3-14. 29. Brome, C.A., Physical and chemical methods of sugar analysis. 1948: p. 895. 30. Reese, E.T. and Mandels, M., Methods in carbohydreate chemistry, ed. Whistler, R.L.ed. 1963, New York: Academic. 139. 31. Nelson, N.A., Photometric adaptation of the somogyi method for the determination of glucose. Journal of Biological Chemistry 1944. 153: p. 375. 32. Ashwell, G., Methods in enzymology, ed. Colowick et al. 3. 1957, New York: Academic Press.p. 85. 33. Dubis et al.., Colorimetric method for determination of sugar and related substances. Analytical and Bioanalytical Chemistry 1956. 28: p. 350-355. 34. Marshall, R.D., Neuberger, A., and Gottschalk et al., Amsterdam:Elsevier Publishing Company. 1972: p. 283-290. 35. Papadopoulas, N.M. and Hess, W.C., Determination of neuraminic (sialic) acid, glucose and fructose is spinal fluid. Archives of Biochemistry and Biophysics, 1960.88: p. 167. 36. 唐國敏, 葡萄糖氧化酶法測定糖化酶活力. 5(4). 1978: 微生物學通報. p.33-34. 37. Yang, F., Ke, Y., and Kuo, S., Effect of fatty acids on the mycelial growth and polysaccharide formation by Ganoderma lucidum in shake flask cultures. Enzyme and Microbial Technology. 2000. 27: p. 295-301. 38. Choi, D.B., Tamura, S., Park, Y.S., Okabe, M., Seriu, Y., and Takeda, S., Efficient Tylosin Production from Streptomyces fradiae Using Rapeseed Oil. Jourmal of Fermentation Technology, 1996. 82(2 ): p. 183-186. 39. 周碧溫, 以饋料批次醱酵對舞菇多醣生產之探討. 2003: 大葉大學食品工程系碩士論文. 40. 吳國偉, 以饋料批次醱酵進行蟲草菌(CCRC36412)多醣生產之研究. 2003: 大葉大學食品工程系碩士論文. 41. Yang, F.C. and Liau, C.B., The influence of environmental conditions on polysaccharide formation by Ganoderma lucidum in submerged cultures. Process Biochemistry, 1998. 33: p. 547-553. 42. 林偉誠, 應用Streptomyces padanus PMS-702 生產fungichromin最適化條件之探討. 2004: 國立中興大學化學工程學系碩士論文. 43. Vu-Trong K., Bhuwapathanapun, S. and Gray, P. P., Metabolic regulation in Tylosin-producing Streptomyces fradiae: phosphate control of Tylosin biosynthesis. . Antimicrobial Agent and Chemistry, 1981. 19(2): p.209-221. 44. Liu, C.M. and Schaffner, C.P., Factors Affecting the Production of Candicidin. Arobl. Lagents and Chemotherapy 1974. 7(2): p. 196-202. 45. Yagi, S., Kitai, S., and Kimura, T., Stimulative effect of elemental Sulfur on Siomycin production by Streptomyces sioyaensis. Applied Microbiology and Biotechnology 1971. 21(2): p. 153-156. 46. Shu C.H., L.M.Y., Effect of pH on the production and molecular wolecular weight distribution of exopolysaccharide by Antrodia camphorata in batch cultures. Process Biochemistry, 2004. 39: p. 931-937. 47. 徐敬衡;林克融, 第七屆生化工程研討會論文集. Effects of pH on the Polysaccharide and Antitumor Activety by Agaricus blazei Murill. 48. Lee, K.M., Lee, S.Y., and Lee, H.Y., Bistage control of pH for improving exopolysaccharide production from mycelia of Ganoderma lucidum in an air-lift fermentor. Journal of Bioscience and Bioengineering 1999. 88: p. 646-650. 49. Fang, Q.H. and Zhong, J.J., Effect of initial pH on production of ganoderic acid amd polysaccharide by submerged fermentation of Ganoderma lucidum. Process Biochemistry, 2002. 37: p. 769-774. 50. Hsieh, C., Hsu, T.H., and Yang, F.C., Production of exopolysaccharides of Ganoderma lucidum(CCRC36021) by reusing thin stillage Process Biochemistry, 2005. 40: p. 909-916. 51. Park, J.P., Kim, S.W., Hwang, H.J., Cho, Y.J., and Yun, J.W., Stimulatory effect of plant oils and fatty acids on the exo-biopolymer production in Cordyceps militaris. Enzyme and Microbial Technology 2002. 31: p. 250-255. 52. Melhuish, J. H. J., Hacskaylo, E., and Bean, G.A., Fatty acid composition of ectomycorrhizal fungi in vitro. Mycologia, 1975. 67: p. 952-960. 53. Sarubbo, L. A., Farias, C. B., and Campos-Takaki, G. M., Co-Utilization of Canola Oil and Glucose on the Production of a Surfactant by Candida lipolytica. Current Microbiology, 2007. 54: p. 68-73. 54. SaKuria, M. and Mori, T., Stimulation of anthocyanin synthesis by conditioned medium produced by strawberry suspension culture. Journal of Plant Physiology 1996. 149: p. 599-604. 55. Kanok-on, W., Penporn. S.T., Hui, Y., Jin, H., Somchai, C., and Zhong, J.J., Impact of conditioned medium on cell culture of Panax notoginseng in an airlift bioreactor. Process Biochemistry, 2001. 37: p. 209-213.
摘要: 中文摘要 靈芝(Ganoderma lucidum)是中藥上常用的一種藥用真菌,根據文獻指出,其所生產之胞外多醣體具有抗腫瘤之療效。本研究利用界面劑和油脂的添加進行靈芝菌絲體之深層培養,進而提升菌體乾重及多醣體之產量。 研究發現,界面劑Span 80的添加對於菌體乾重和多醣體的產量皆具提升效果,添加2%Span80之菌體乾重為9.41 g/L,為未添加的1.72倍,同時多醣體的產量由341.99 mg/L 提升至 536.92 mg/L;在油脂的添加方面,橄欖油的添加有助於菌體乾重的提升,而玉米油的添加對於多醣體的產量是有提升的效果為未添加的2.1倍。於第0天添加2%玉米油、1%橄欖油及起始pH值6下,以轉速100 rpm的條件進行培養,其結果發現,菌體乾重及多醣體之產量都有提升的效果,多醣體的產量約為未添加時的4.74倍,而菌體乾重約為未添加時的3倍;另一方面,於第12天提昇轉速至200 rpm,其多醣體產量於第13天可達1810 mg/L。
Abstract Ganoderma lucidum is one of the most famous traditional Chinese medicinal herbs. It was reported that polysaccharide isolated from fruiting body and the mycelia culture of G. lucidum has immunomodulating and anti-tumor activity. The object of this study was to enhance mycelia and polysaccharide production in submerged culture by addition of surfactants or oils. In supplementation of surfactant experiments, Span 80 have positive effect on dry cell concentration and polysaccharide production. The dry cell concentration was 9.41g/L by addition of 2% Span 80, 1.72 times to that of control. Meanwhile, the polysaccharide production was enhanced from 341.99mg/L to 536.92mg/L. In experiments of oils supplementation, addition of olive oil was favor to mycelia growth. In contrast, supplementation of the corn oil increased the polysaccharide production (2.1 times compared to control). Based on the above results, a fermentation was carried out by adding 2% corn oil and 1% olive oil, and the initial pH and rotation speed was 6 and 100 rpm, respectively. The results showed that the mycelia as well as polysaccharide production was dramatically improved (3.0 and 4.74 times compared to control respectively). On the other hand, to enhance polysaccharide production, experiments were conducted by adiusting the rotation rates to alter the share force excenting on the strain. When the rotation rate was changed from 100 to 200 rpm at 12 days' cultivation, the results showed that polysaccharide production increased to 1810 mg/L at day 13, a 5.3 times increase as compared to that of control.
URI: http://hdl.handle.net/11455/3614
其他識別: U0005-0607200717381700
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-0607200717381700


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。