Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36223
標題: 百合花粉與蘇鐵雌配子體中油體蛋白的基因選殖與分析
Cloning and analyses of oil-body proteins in lily pollen and cycad megagametophytes
作者: 江佩倫
Jiang, Pei-Luen
關鍵字: Lily
百合
Cycad
Oil bodies
Oleosin
Caleosin
Megagametophyte
Pollen
Germination
蘇鐵
油體
油體膜蛋白
油體鈣蛋白
雌配子體
花粉
花粉萌發
出版社: 生物科技學研究所
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摘要: Stable oil bodies were purified from mature lily (Lilium longiflorum Thunb.) pollen. Immunodetection revealed that a major protein of 18 kDa was exclusively present in pollen oil bodies. Sequence analysis as well as immunological non-cross recognition suggests that this pollen oleosin represents a distinct class in comparison with oleosins found in seed oil bodies and tapetum. Similar to oleosin, a putative caleosin was specifically detected in pollen oil bodies. Sequence alignment of seed and pollen caleosins as well as promoter analysis of rice oleosin and caleosin genes suggested that caleosins in pollen oil bodes represented a distinct class compared with those in seed oil bodies. In pollen cells observed under electron microscopy, oil bodies were presumably surrounded by tubular membrane structures, and encapsulated in the vacuoles after germination. It seems that pollen oil bodies are mobilized via a different route in contrast with the glyoxysomal mobilization of seed oil bodies after germination. Furthermore, stable oil bodies were isolated from mature cycad (Cycas revoluta) megagametophytes. Immunological cross-recognition revealed that cycad oil bodies contained a major protein of 27 kDa, tentatively identified as caleosin, while oleosin, the well-known structural protein, was apparently absent. Stable artificial oil bodies were successfully constituted with triacylglycerol, phospholipid and the recombinant fusion protein containing the cycad caleosin. Therefore, the results suggested that stable oil bodies in cycad megagametophytes are mainly sheltered by a unique structural protein caleosin.
URI: http://hdl.handle.net/11455/36223
其他識別: U0005-2406200914260100
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2406200914260100
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