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dc.contributorHung-Ming Yungen_US
dc.contributorYen-Hui Chenen_US
dc.contributorHsin-Fu Changen_US
dc.contributorFei-Lung Wangen_US
dc.contributor.advisorChi-Min Chengen_US
dc.contributor.authorLiu, You-Chenen_US
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dc.description.abstract本研究目的為製備奈米碳管-黏土/Nafion 薄膜修飾玻璃碳電極,並將電極應用於偵測過氧化氫。在pH=7 的磷酸緩衝溶液下,奈米碳管-黏土/Nafion 薄膜修飾玻璃碳電極的操作電壓為-0.6V。當過氧化氫累積濃度為12.8mM 時,奈米碳管-黏土/Nafion 薄膜修飾玻璃碳電極能產生較高的電流為136.87μA。且在掃描速率為25 到200mV/s,整個電催化過程為表面控制。 而奈米碳管-黏土/Nafion 薄膜修飾玻璃碳電極, 在含有8mg/ml 的 CNTs-Clay 量,和取6μl的CNTs-Clay/Nafion 溶液以及掃描速率為200rpm的最佳條件下,其靈敏度為10942 nA/mM,應答時間為5 秒,偵測範圍為0.1~12.8mM 的過氧化氫。 在葡萄糖偵測方面,當葡萄糖氧化酵素濃度為2mg/ml 時,靈敏度顯 示為1718nA/mM,線性範圍0.2~1.3mM,應答時間為10 秒,而葡萄糖濃度在2.8mM 時,應答電流為3.02μA。 關鍵字:生物感測器,黏土,奈米碳管zh_TW
dc.description.abstractThe preparation of biosensors based on the carbon nanotubes (CNTs)-Clay/Nafion coated on the thin film glassy carbon electrode (GCE) fordetecting the hydrogen peroxide (H2O2) was investigated. The CNTs-Clay/Nafion with a operating potential of -0.6 V in 0.1 M, pH 7.0 phosphate buffer solution. The superior performance of higher current of 136.87 μA with the accumulation of 12.8mM H2O2 was observed as the CNTs-Clay/Nafion modified glassy carbon electrode. The response has shown a surface-controlled electrocatalyze process determined in the scan rate range from 25 to 200 mV/s. Under the optimal conditions of 8mg/ml CNTs-Clay, 6μl CNTs-Clay/Nafion solution, pH 7.0 phosphate buffer solution and agitation speed of 200rpm, the sensitivity and response time of the CNTs-Clay/Nafion/GCE biosensor were 10942 nA/mM and 5 seconds,respectively, in the detecting range of 0.1~12.8mM H2O2 . The resulting sensor displays a high sensitivity (1718nA/mM) and a linear range from 0.2 to 1.3 mM, the response time was 10 seconds for glucose determination, the current of 3.02 μA with the accumulation of 2.8mM glucose. Key words: biosensors, clay, carbon nanotubesen_US
dc.description.tableofcontents摘要 I Abstract II 目錄 III 表目錄 V 圖目錄 VI 第一章 前言 1 第二章 文獻回顧 2 (一) 生物感測器(Biosensor) 2 1. 生物感測器的工作原理 2 2. 生物感測器的組成 2 3. 生物感測器需具有的優點 3 (二) 電化學原理 5 1. 電化學分析 6 (三) 電化學式生物感測器 10 1. 電位式生物感測器(Potentiometric biosensors) 10 2. 電流式生物感測器 (Amperometric biosensors) 10 3. 電阻式生物感測器(Conductrometric biosensors) 11 (四) 化學修飾電極(Chemically Modified Electrodes,CMEs) 12 (五) 奈米材料修飾電極 15 1. 黏土(clay)之簡介 15 (六) 酵素電極 30 1. 葡萄糖氧化酵素之簡介 30 (七) 固定化技術 31 1. 酵素固定化之簡介 31 2. 電極表面之酵素固定法 32 第三章 材料與方法 36 (一) 實驗材料 36 (二) 藥品配製 36 (三) 實驗儀器 37 (四) 實驗步驟 38 (五) 實驗流程 42 第四章 結果與討論 44 (一) 製備Ni-Clay與CNTs-Clay的表面型態 44 1. 場發射掃描式電子顯微鏡(Field emission-scanning electron microscope,FE-SEM) 44 2. X光能量散佈分析儀(X-ray energy dispersive spectrometer,EDS) 46 (二) 不同薄膜修飾玻璃碳電極之表面型態分析 47 1. 場發射掃描式電子顯微鏡(Field emission-scanning electron microscope,FE-SEM) 47 (三) 奈米碳管-黏土/Nafion 薄膜修飾玻璃碳電極偵測過氧化氫 49 1. 循環伏安法 50 2. 安培伏安法 55 (四) CNTs-Clay/Nafion薄膜修飾玻璃碳電極之碳管含量的探討 62 (五) 攪拌速度對CNTs-Clay/Nafion修飾玻璃碳電極的探討 68 (六) 膜厚度對偵測過氧化氫的影響 74 (七) CNTs-Clay/Nafion/葡萄糖氧化酵素(GOD)薄膜修飾玻璃碳電極偵測葡萄糖之試驗 79 第五章 結論與研究方向 83 (一) 結論 83 (二) 研究方向 84 第六章 參考文獻 85zh_TW
dc.subjectcarbon nanotubesen_US
dc.titleApplications of carbon nanotubes synthesized over the Ni particles on clay for Electrochemical biosensoren_US
dc.typeThesis and Dissertationzh_TW
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