Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3634
標題: 大氣電漿改質神經導管材料於周邊神經之應用
The application of nerve conduits materials modified by atmospheric plasma treatment
作者: 林哲永
Lin, Zhe-Yong
關鍵字: Air plasma
接枝
grafing
microgroove
gold nanocomposite
chitosan
PLA
fibroblastic cell growth factor 1
微溝槽
納米金幾丁聚醣
聚乳酸
生長因子
出版社: 化學工程學系所
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摘要: 本研究以生物可降解材料聚乳酸(PLA)做為基材,以微影製程之模板拓印出具方向性溝槽表面的聚乳酸膜材,利用大氣電漿裝置改質具微溝槽聚乳酸表面,以具生物相容性之幾丁聚醣及納米金-混幾丁聚醣做為表面修飾分子,並利用纖維母細胞生長因子接枝於納米金-混幾丁聚醣表面,之後以掃描式電子顯微鏡、化學分析電子光譜儀等表面分析評估改質結果。研究發現,聚乳酸經大氣電漿改質接上幾丁聚醣及納米金-混幾丁聚醣後,化學分析電子光譜及掃描式電子顯微鏡皆可明顯觀察到微溝槽表面的幾丁聚醣及納米金-混幾丁聚醣分子。此外,小鼠神經幹細胞生長增加且細胞排序程度在72小時由原本84.3%提升至89.5%。此外,大氣電漿修飾納米金-混幾丁聚醣的表面後更能增加生長因子的吸附量及提升基因表現,故大氣電漿為一有潛力之生醫材料表面改質方法。
In this study the bridgeable polylactide (PLA) substrate was fabricated with instructive microgrooves first, and then grafted with chitosan-Au nanocomposites (chi-Au) and fibroblast growth factor 1 (FGF1) by atmosphere air plasma treatment to modify the hydrophobic surface. The surface properties of modified substrates were characterized by scanning electron microscope (SEM) and electron spectroscopy for chemical analysis (ESCA). The results showed that the presence of chitosan and chi-Au were demonstrated on PLA surface by SEM and ESCA after atmosphere air plasma treatment. For in vitro studies, the degree of cell alignment and the proliferation of murine neural stem cells were evaluated. It was shown that the cell proliferation was significantly higher on the modified surface and the degree of cell alignment also increased from 84.3% to 89.5% at 72 hours. Furthermore, greater levels of gene expression for BDNF, GDNF and FGF1 and more FGF1 were absorbed on the air plasma modified chi-Au substrate. It is concluded from this study that the use of air plasma was a potential technique for surface modification of biomaterials.
URI: http://hdl.handle.net/11455/3634
其他識別: U0005-2008200713372700
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2008200713372700
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