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Preparation of adsorptive membranes via modification of inorganic membranes and their applications in bioseparation
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|摘要:||本論文分成兩部分: 第一部份研究(以烷基酸分子改質多孔性氧化鋁薄膜及其在蛋白質吸附的應用)，利用不同濃度的正辛酸與正十八酸在不同反應時間下改質多孔性氧化鋁薄膜，以製備疏水性薄膜。續以接觸角儀、全反射傅立葉紅外線光譜儀、與化學分析電子儀分析改質後之薄膜性質，證實改質成功，並獲得較佳改質條件: 10 mM烷基酸濃度與20分鐘的反應時間。本論文更進一步探討在批次與流動程序中，以較佳改質條件下所得之薄膜對牛血清蛋白的吸、脫附表現。批次等溫線結果顯示: 利用正辛酸與正十八酸改質之薄膜，對牛血清蛋白的飽和吸附量分別約為0.003及0.004 micron mol/cm2。此外，本研究亦利用液相層析儀與螢光儀探討牛血清蛋白在吸、脫附過程中可能的構型變化。另流動程序的實驗結果顯示: 牛血清蛋白緩慢的吸、脫附速率為影響其流動吸、脫附成效的主因。
第二部份的研究(製備無機-有機混成陰離子交換薄膜及其在質體DNA與RNA分離之應用)，則是將N-[3-(trimethoxysilyl)propyl] ethylene diamine 與3-(triethoxysilyl)propyl isocyanate反應後產物(前驅物)塗佈至多孔性玻璃纖維及氧化鋁薄膜，再經溴乙烷反應，以製備無機-有機混成陰離子交換薄膜，並利用傅立葉紅外線光譜儀分析結果證實薄膜改質成功。經改質後，玻璃纖維及氧化鋁薄膜的陰離子交換容量分別為6.2 及 1.5 micron eq/cm2。批次吸附量結果顯示:質體DNA吸附量高低排序為商業高分子薄膜產品SB6407 > 改質後玻璃纖維薄膜 > 改質後氧化鋁薄膜；RNA吸附量高低排序則為SB6407≒改質後玻璃纖維薄膜 > 改質後氧化鋁薄膜。另外，最佳批次脫附條件為二階段脫附:利用含有2 M NaCl的50 mM Tris-HCl, pH 8溶液脫附RNA，再以含1 M NaCl及20% ethanol的50 mM Tris-HCl, pH 8溶液脫附質體DNA。在流動程序中，當流速為1 ml/min、進料為10 micron g質體DNA + 10 micron g RNA的混合物或cell lysate時，以一片改質後玻璃纖維薄膜或SB6407薄膜(直徑47 mm)可將質體DNA及RNA完全分離，但使用相疊多片的改質後氧化鋁薄膜(直徑13 mm)則分離效果差。使用改質後玻璃纖維薄膜所得質體DNA的回收率可達98-106%，高於SB6407薄膜的回收率(91-96%)。|
This study was divided into two parts. In the first part (Modification of porous alumina membranes with n-alkanoic acids and their application in protein adsorption), porous alumina membranes were modified with two kinds of n-alkanoic acids (octanoic acid and octadecanoic acid) at different concentrations and reaction times for the preparation of hydrophobic membranes. The properties of the modified membranes were characterized by contact angle, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), and electron spectroscopy for chemical analysis (ESCA), and the better modification condition was determined as reaction time of 20 min and concentration of 10 mM for both n-alkanoic acids. Furthermore, adsorption and desorption performances of bovine serum albumin (BSA) onto the modified membranes in the batch and flow processes were investigated. The saturation capacity was 0.003 micron mol/cm2 for the octanoic acid-modified membrane and 0.004 micron mol/cm2 for the octadecanoic acid-modified membrane, under the better modification condition. Liquid chromatography and fluorescence measurement were subsequently conducted to analyze the BSA properties during the adsorption and desorption stages. Finally, the flow performance of BSA was found to be limited by its slow adsorption and desorption rates. In the second part of this study (Preparation of inorganic-organic anion-exchange membranes and their application in plasmid DNA and RNA separation), inorganic-organic anion-exchange membranes were prepared by coating a precursor, the product of N-[3-(trimethoxysilyl)propyl] ethylene diamine reacted with 3-(triethoxysilyl)propyl isocyanate, on macroporous glass fiber and alumina membranes, followed by bromoethane treatment. The FTIR results demonstrated the successful membrane modification, and the resulted anion-exchange capacities were 6.2 and 1.5 micron eq/cm2, respectively, for modified glass fiber membrane and modified alumina membrane. In batch adsorption process, the corresponding adsorption capacity for plasmid DNA could be sorted by: commercial polymeric SB6407 > modified glass fiber > modified alumina membrane; while for RNA adsorption, the order became: modified glass fiber = SB6407 > modified alumina membrane. The optimal elution condition found from batch desorption performance was: 2 M NaCl in 50 mM Tris-HCl, pH 8 for RNA elution, followed by 1 M NaCl and 20% ethanol in 50 mM Tris-HCl, pH 8 for plasmid DNA elution. In membrane chromatography process, plasmid DNA and RNA could be clearly separated from the feed of 10 micron g plasmid DNA + 10 micron g RNA mixture or cell lysate by one piece of 47 mm modified glass fiber or SB6407 membrane, but not by the stacked 13 mm modified alumina membranes. The overall plasmid DNA recovery for the modified glass fiber membrane was 98-106%, higher than that of SB6407 membrane (91-96%).
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