請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/36555
標題: Lipid-mediated preferential localization of hypericin in lipid membranes
作者: Ho, Y.F.
施明智
Wu, M.H.
Cheng, B.H.
Chen, Y.W.
Shih, M.C.
關鍵字: Hypericin
Cholesterol
Giant unilamellar vesicle
Phase separation
Subcellular distribution
Photodynamic therapy
photodynamic therapy
subcellular-localization
ternary mixtures
sterol
transport
carcinoma cells
cancer-cells
in-vitro
cholesterol
phospholipids
rafts
期刊/報告no:: Biochimica Et Biophysica Acta-Biomembranes, Volume 1788, Issue 6, Page(s) 1287-1295.
摘要: Subcellular localization of a photosensitizer is critical to its therapeutic outcome during photodynamic therapy (PDT). We delineated the distribution of hypericin, a new generation photosensitizer, in model membrane systems to identify the operating principles of its subcellular accumulation. Results from fluorescence microscopy indicated preferential incorporation of hypericin in lipid of giant unilamellar vesicles. Monolayer fluorescence measurements further identified cholesterol as the key determinant for the observed selectivity of hypericin. The emission spectra of hypericin in lipid monolayers varied in a lipid-dependent manner and Stoke's shift behavior suggests that hypericin may form closely packed structure with cholesterol. Overall, our data lead to the conclusion that cholesterol is the major origin of the selectivity for hypericin in membrane systems. A hypothetical model depicting the intracellular and intravascular cotransport of hypericin and cholesterol because of their high affinity is presented. (c) 2009 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/36555
ISSN: 0005-2736
文章連結: http://dx.doi.org/10.1016/j.bbamem.2009.01.017
顯示於類別:物理學系所

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