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標題: 水稻T-DNA插入突變體抗固殺草之生理機制
Physiological mechanism of glufosinate resistance for T-DNA insertion mutants of rice
作者: 蕭詩軒
Hsiao, Shi-Xuan
關鍵字: glufosinate
glutamine synthetase
出版社: 農藝學系所
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摘要: 本研究利用水稻T-DNA插入突變庫之抗性品系084-5-1-1(R)與感性品系283-0-4(S),並配合富士光(FSK)為對照,探討固殺草之抗性生理機制。根據劑量反應分析與處理7.54 mM 固殺草7天後的存活率,顯示084-5-1-1(R)表現較佳的抗性。於利用14C-glufosinate標定後48小時,發現在固殺草的吸收轉運上,各品系間並無明顯差異。但084-5-1-1(R)具有較高之代謝能力,其代謝物主要是MPP。研究發現噴施固殺草之後,初期084-5-1-1(R)植體內GS酵素(GS; EC維持較高的活性且受抑制程度較低,且24小時後銨累積量亦較低。根據酵素動力學分析,084-5-1-1(R)品系於細胞液內的GS有較高之Ki值,顯示對於固殺草親和力降低,此可能有助於其抗性。
The physiological basis of glufosinate resistance for the rice mutants derived from T-DNA insertion-induced mutant pool, resistant (R) line 084-5-1-1(R) , as compared with susceptible (S) mutant line 283-0-4 (S) and a reference variety FSK, was studied. Based on the dose-response analysis and survival rate of 4-leaf rice seedlings 7 days after treatment with 7.54 mM glufosinate, mutant line 084-5-1-1(R) expressed a higher resistante to glufosinate. A 14C-glufosinate feeding experiment showed no difference among 3 rice lines in both uptake and translocation of glufosinate. However, a higher glufosinate metabolism in R line 084-5-1-1 was found. In addition, in vivo activity of glutamine synthetase (GS; EC in R line was higher and it was decreased slowly at the beginning after glufosinate treatment. Due to the higher Ki value to glufosinate for the cytosolic GS based on the enzymatic kinetic analysis, the decreased affinity of cytosolic GS to glufosinate could partially account for the glufosinate resistance in R line 084-5-1-1(R) from T-DNA insertion mutants. It is suggested that both a higher metabolism of glufosinate and a lower affinity of cytosolic GS to glufosinate are main factors to resistant glufosinate.
其他識別: U0005-2706200614005500
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