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標題: 水稻突變體對巴拉刈除草劑反應差異性之研究
Study on the differential responses of rice mutants to paraquat
作者: 歐人輔
Ou, Jen-Fu
關鍵字: rice
chlorophyll fluorescence
出版社: 農藝學系所
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摘要: 本研究主要針對水稻台農67號經由疊氮化鈉(NaN3)誘變所得之1,343個突變體(M7),篩選對巴拉刈(paraquat)具抗、感性之突變體。在巴拉刈處理下,根據水稻台農67號及富士光V4期幼苗的傷害指數與黃化百分比,利用非線性回歸分析之log-logistic模式,由水稻對巴拉刈之劑量反應(dose-response)分析,推估引起植株傷害達50%之藥劑濃度(ED50),作為抗、感性突變體之篩選劑量。調查供試之1,343個突變體在0.01 mM巴拉刈處理後之傷害指數,初步選出較具巴拉刈感性(PS)之6個品系,分別為81、802、875、948、1081及1192。之後分別以0.1及0.2 mM巴拉刈處理,篩選出較具巴拉刈抗性(PR)之7個品系,包括:72、713、881、882、883、986及1067。另進行抗、感性突變品系分蘗期葉片片段之傷害指數調查,發現PR-72與PS-1192較其他突變品系具有較大抗、感性。本研究進一步利用葉綠素螢光分析確立世代間與品系間之抗、感程度差異。研究顯示不同世代間,在巴拉刈處理下抗性突變品系PR-72的光系統II光化學最大效能(Fv/Fm)表現皆顯著高於感性突變品系PS-1192。進一步分析抗、感性突變品系之最小螢光量(Fo)及最大螢光量(Fm),顯示在巴拉刈處理下,感性突變體之光系統II反應中心電子傳遞效率顯著下降。 本研究經篩選得到M9世代之72-16-4與1192-11-5兩個品系,以光系統II光化學最大效能表現之相對頻度分析,顯示巴拉刈處理下,依抗、感性突變體對巴拉刈抗性表現可區分為兩族群,其中PS-1192在不同世代的表現較感性,而PR-72則表現較抗性,且抗、感族群世代間表現相近。本研究將進一步以抗性品系72-16-4及感性品系1192-11-5進行雜交後代抗性表現分析,俾了解巴拉刈抗性之遺傳特性。
Paraquat-resistant (R) and -susceptible (S) rice plants were screened successfully from 1,343 rice mutants (M7), derived from sodium azide (NaN3) treatment, in this study. Screening system was established based on the dose-response analysis of rice cv. Tainung 67 and Japanese cv. FSK to paraquat, and the ED50 of around 0.1 mM paraquat observed has been used as a reference dose for subsequent screening process. According to injury index and yellow percentage of rice seedlings treated with paraquat at V4 stage, six S lines (81, 802, 875, 948, 1081 & 1192) and seven R lines (72, 713, 881, 882, 883, 986 & 1067) were firstly selected. Further screening at tillering stage based on the injury of leaf segment showed that the line 72 and 1192 were the most resistant and susceptible lines, respectively. In order to ensure the accuracy of paraquat resistance measurement, the instrumental assay, the chlorophyll fluorescence determination, was used to detect the differential changes of paraquat damage between R and S lines. Measurement of the maximal efficiency of PSII photochemistry (Fv/Fm) found that the line 72 was more resistant to paraquat than the line 1192, and this change was maintained between two generations (M7 and M8). Further screening process and comparison of paraquat resistance between two generations (M8 and M9) similar to the method described above confirmed that the line 72-16-4 and 1192-11-5 were the most resistant and susceptible lines, respectively, at the generation M9, and the genetic behavior of paraquat resistance we obtained in this study will be explored through the hybridization and back-cross test in the near future.
其他識別: U0005-0502200916082400
Appears in Collections:農藝學系



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